Photo of Leslie M. Loew, Ph.D.

Leslie M. Loew, Ph.D.

Professor, Department of Cell Biology
Professor of Computer Science and Engineering
Director, Center for Cell Analysis and Modeling
Editor in Chief, Biophysical Journal
Academic Office Location:
Cell Biology
UConn Health
263 Farmington Avenue
Farmington, CT 06030-6406
Phone: 860-679-3568
Fax: 860-679-1039
Email: les@volt.uchc.edu
Website(s):

Les Loew Quick Bio

Richard D. Berlin Center for Cell Analysis and Modeling

Virtual Cell

Cell Analysis & Modeling Graduate Program

Cell Biology Graduate Program

Neuroscience Graduate Program

Education
DegreeInstitutionMajor
B.S.City College of CUNYChemistry
M.S.Cornell UniversityChemistry
Ph.D.Cornell UniversityChemistry

Post-Graduate Training
TrainingInstitutionSpecialty
PostdoctoralHarvard University Chemistry

Awards
Name of Award/HonorAwarding Organization
Editor-in-Chief, Biophysical JournalBiophysical Society
Elected to Connecticut Academy of Science and Engineering (CASE) CASE
Chair NIH MABS Study Section, 2004-2007NIH
Boehringer Ingelheim Endowed Chair in Cell SciencesUniversity of Connecticut Health Center
NIH Research Career Development AwardNIH
Research Career Development AwardNIH
Phi Beta Kappa, Nehemiah Gittelson MedalPBK
Name & DescriptionCategoryRoleTypeScopeStart YearEnd Year
Biophysical JournalProfessional/Scientific JournalEditor in ChiefExternalNational20122017
Search Committee for Exec VP of UCHC and Dean of the Medical SchoolResearch CommitteeMemberUConn HealthLocal20112012
Site visit review team for a NIH/NCRR Biomedical Technology Research Resource grant at U. Washington, Seattle, WAAdvisory CommitteeMemberExternalNational20112011
Computational Cell Biology Meeting, Cold Spring Harbor Laboratory, Long Island, NYAdvisory CommitteeCo-Organizer ExternalNational20112011
Biophysical JournalProfessional/Scientific JournalAssociate EditorExternalNational20102012
Course & Curriculum Evaluation Subcommittee Education CommitteeMemberUConn HealthLocal20102011
NIH Director Pioneer Awards ReviewerAdvisory CommitteeMemberExternalNational20102010
Computational Cell Biology Meeting, Wellcome Trust Genome Campus, Hinxton UKAdvisory CommitteeCo-Organizer ExternalInternational20102010
LCME Institutional Setting Self Study Committee, Research subcommitteeResearch CommitteeChairUConn HealthLocal20092009
UCONN Booth Engineering Center for Advanced Technology (BECAT) Oversight Committee Education CommitteeMemberUConn HealthLocal2009
Nanomedicine Faculty Search CommitteeEducation CommitteeChairUConn HealthLocal20092010
Dept. of Surgery Chair Search Committee Education CommitteeChairUConn HealthLocal20092011
Vascular Biology Center Director Search Committee Research CommitteeMemberUConn HealthLocal20092010
Research Recruitment Committee Education CommitteeMemberUConn HealthLocal2009
Computational Cell Biology Meeting, Cold Spring Harbor Laboratory, Long IslandAdvisory CommitteeCo-Organizer ExternalNational20092009
Site visit review team for a NIH/NCRR Biomedical Technology Research Resource grant at UCLAAdvisory CommitteeMemberExternalNational20092009
Connecticut Academy of Science and EngineeringProfessional/Scientific OrganizationMemberExternalLocal2008
UCONN Search Committee for VP Research & Dean of Grad School Research CommitteeMemberExternalLocal20082008
UCHC Scientific Advisory Committee for the Clinical and Translational Science Institute Advisory CommitteeMemberUConn HealthLocal20082009
Computational Cell Biology Meeting at HinxtonAdvisory CommitteeCo-Organizer ExternalNational20082008
NIH Biomedical Technology Research Resources Directors MeetingAdvisory CommitteeChair of Organizing Committee ExternalNational20082008
IET Systems BiologyEditorial BoardExternalNational2007
School of Medicine Dean’s Council OtherMemberUConn HealthLocal20072008
400 Farmington Building Committee Advisory CommitteeMemberUConn HealthLocal2007
Computational Cell Biology Meeting at Cold Spring Harbor LaboratoryAdvisory CommitteeCo-Organizer ExternalNational20072007
Biophotonics Faculty Search Committee Research CommitteeChairUConn HealthLocal2005
School of Medicine Research Council Research CommitteeMemberUConn HealthLocal20052008
Biophysical Society Annual Meeting Program CommitteeAdvisory CommitteeMember ExternalNational20052007
NIH Modeling and Analysis of Biological Systems Study SectionStudy SectionChairExternalNational20052007
External Scientific Advisory Board for National Resource for Biomedical Supercomputing Advisory CommitteeChairExternalNational2005
External Scientific Advisory Board for National Biomedical Computation Resource Advisory CommitteeMemberExternalNational2005
NIH Modeling and Analysis of Biological SystemsStudy SectionChairExternalNational20042007
New Research Building Planning Committee Advisory CommitteeMemberUConn HealthLocal20042004
Research Building Planning Group Advisory CommitteeMemberUConn HealthLocal20042005
Institutional Research Policy Advisory Committee Advisory CommitteeMemberUConn HealthLocal20042006
Research Computing Committee Research CommitteeMemberUConn HealthLocal2004
NIH Modeling and Analysis of Biological Systems Study Section Study SectionMemberExternalNational20042005
Research Services Enhancement Working Group Education CommitteeMemberUConn HealthLocal20032004
Computational Cell Biology Faculty Search Committee Research CommitteeMemberUConn HealthLocal20032004
NIH Systems Biology Special Interest Group Advisory CommitteeAdvisorExternalNational20032003
Ad Hoc Faculty Grievance Committee Education CommitteeMemberUConn HealthLocal20022002
Structural Biology Faculty Search Committee Research CommitteeMemberUConn HealthLocal20022003
NCRR Computational Cell Biology Working Group Advisory CommitteeChairExternalNational20022002
Computational Cell Biology Faculty Search Committee Research CommitteeMemberUConn HealthLocal20012002
International Symposium on Computational Cell Biology, Lenox, MAAdvisory CommitteeOrganizerExternalNational20012005
NIH Center For Scientific Review, Bioengineering Sciences and Technologies (BEST) IRG Formation CommitteeProfessional/Scientific OrganizationMemberExternalNational20012001
MRC(London)/NIGMS Joint workshop on Computational Cell BiologyWorkshop/ConferenceMemberExternalInternational20012001
DSRC Workshop on Functional Integration of Cells and MaterialsWorkshop/ConferenceMemberExternalNational20012001
Biophysical Society Publications Committee on Interactive Electronic PublicationAdvisory CommitteeChairExternalNational20002004
DARPA Focus 2000 Workshop/ConferenceCo-ChairExternalNational20002000
NIDA Workshop on Cell Biology of AddictionWorkshop/ConferenceMemberExternalNational20002000
Workshop on Computational Cell Biology, Biophysical Society Annual Meeting, New OrleansAdvisory CommitteeChairExternalNational19991999
NIGMS Development of Interactive DatabasesWorkshop/ConferenceMemberExternalNational19991999
NIGMS Training in Computational Biology /BioinfomaticsWorkshop/ConferenceMemberExternalNational19991999
Neuroscience Chairperson Search Committee Research CommitteeMemberUConn HealthLocal19981999
Biophysical JournalEditorial BoardExternalNational19972006
Journal of FluorescenceEditorial BoardExternalNational19972002
Cell Biochemistry and BiophysicsEditorial BoardExternalNational1997
Center for Science and Technology Commercialization Advisory CommitteeMemberUConn HealthLocal1997
Special Task Force on Research Funding OtherMemberUConn HealthLocal19961997
Health Center Research Advisory Committee Advisory CommitteeMemberUConn HealthLocal19952000
Information Systems Advisory Committee Education CommitteeMemberUConn HealthLocal19941996
Senior Appointments & Promotions Committee Advisory CommitteeMemberUConn HealthLocal19932006
Interdepartmental Basic Sciences Seminar Committee Education CommitteeMemberUConn HealthLocal19921995
Medical School Council Education CommitteeMemberUConn HealthLocal19901992
Interdepartmental Basic Sciences Seminar Committee Education CommitteeChairUConn HealthLocal19891992
NCRR Integrated Genomics TechnologiesWorkshop/ConferenceMemberExternalNational19881988
Graduate Programs Committee Education CommitteeMemberUConn HealthLocal19861990
Cell Biology Graduate Program Education CommitteeChairmanUConn HealthLocal19861990
Cell Biology Graduate Program Steering committee Education CommitteeMemberUConn HealthLocal19862001

Research in this laboratory encompasses several projects. We have a longstanding effort aimed at developing and characterizing fluorescent probes of membrane potential. Although this effort is continuing, we are now particularly focused on studies of the non-linear optical properties of the dyes, including second harmonic generation as an alternative to fluorescence as a probe of membrane potential. This work has led to a continual interest in the organization of signaling pathways along cell membrane and within the cytoplasm. We are interested in the very general question of how the intricate spatial organization of molecules in cells is used to control cell function. This is especially true of neuronal cells and we are actively investigating the cell biology of dendritic spines in brain tissue. Finally, we are very excited about a project called the "Virtual Cell", in which we have created a framework for using computer simulation to explore cell biological mechanisms. The models are built naturally from experimental images of cell and subcellular structures combined with biochemical and electrophysiological data. We have used the “Virtual Cell” system are to explain the pattern of electrical and signaling activity in neuronal and non-neuronal cells.

Accepting Lab Rotation Students: Summer '16, Fall '16, Spring '17


Lab Rotation Projects
We are interested in the initial electrical and calcium signals that initiate long-term changes in the synaptic strength of dendritic spines. We will use 2-photon microscope imaging of fluorescent indicators along with patch clamp recording to measure these signals. Rotation projects could involve either experimental work on the calcium imaging or computational modeling with the Virtual Cell software related to how the morphology of the spine regulates calcium signals.

Journal Articles

Books

  • Loew LM (ed) Spectroscopic Membrane Probes, Vols. I, II and III 1988 Jan;

Book Chapters

  • The role of amoebapore in contact-mediated cytolysis
    Rosenberg IM, Bach D, Loew LM, Gitler C Amoebiasis. Human Infection by Entaboeba Hystolytica 1988 Jan;

Book Sections

  • Monitoring Membrane Potential with Second Harmonic Generation
    Wilson SA, Millard AC, Lewis A, Loew LM Imaging A Laboratory Manual 2011 Jan;721-734
  • Assessment of cardiac conduction: basic principles of optical mapping.
    Ding, Chunhua; Everett, Thomas H Methods in molecular biology (Clifton, N.J.) 2010 Jan;660239-52
  • Design and Use of Organic Voltage Sensitive Dyes
    Loew LM Membrane Potential Imaging in the Nervous System: Methods and Applications 2010 Jan;
  • Second Harmonic Imaging of Membrane Potential
    Loew LM Membrane Potential Imaging in the Nervous System: Methods and Applications 2010 Jan;
  • The Virtual Cell Project
    Loew LM, Schaff JC, Slepchenko BM, Moraru II Systems Biomedicine 2010 Jan;273-288
  • Imaging Membrane Potential with Voltage-Sensitive Dyes
    Vucinic D, Kosmidis EK, Falk CX, Cohen LB, Loew LM, Djurisic M, Zecevic D Biomedical Optical Imaging 2009 Jan;132-160
  • The correlation between photosensitizers' membrane localization, membrane-residing targets and photosensitization efficiency
    Ytzhak S, Bernstein S, Loew LM, Ehrenberg B Photodynamic Therapy: Back to the Future. Vol. 7380 2009 Jan;738029-1 - 738029-10
  • The Virtual Cell Project
    Loew LM ‘In Silico’ Simulation of Biological Processes: Novartis Foundation Symposium 247 2002 Jan;151-161
  • Fluorescent staining of subcellular organelles and structures
    Terasaki M, Lippincott-Schwartz J, Zaal KJ, Loew LM Current protocols in cell biology 1999 Jan;
  • Potentiometric dyes and imaging membrane potential in single cells
    Loew LM Fluorescent and luminescent probes for biological activity 1999 Jan;210-221
  • Measuring membrane potential in single cells with confocal microscopy
    Loew LM Cell Biology: A Laboratory Handbook Vol. 3 1998 Jan;375-379
  • Characterization of potentiometric membrane dyes
    Loew LM Membrane Electrochemistry 1994 Jan;151-173
  • Measuring membrane potential in single cells with confocal microscopy
    Loew LM Cell Biology: A Laboratory Handbook 1994 Jan;399-403
  • Potentiometric membrane dyes
    Loew LM Fluorescent and Luminescent Probes for Biological Activity. A Practical Guide for Quantitative Real-Time Analysis 1993 Jan;150-160
  • The electrical properties of membranes
    Loew LM Handbook of Biomembranes, Vol. 1 1993 Jan;341-371
  • Membrane potential imaging
    Loew LM New Techniques of Optical Microscopy and Microspectrophotometry 1991 Jan;255-272
  • Action on artificial and neuronal membranes of Paraxin, a new presynaptic excitatory polypeptide neurotoxin with ionophore activity
    Lazarovici P, Primor M, Caratsch CG, Manz K, Lelkes P, Loew LM, Shai Y, McPhie P, Louini A, Contreras ML, Fox J, Shih YL, Edwards C Neurotoxins as Tools in Neurochemistry 1989 Jan;
  • Optical measurement of membrane potential in invertebrate ganglia and mammalian cortex
    Wu J.-y., London JA, Zecevic D, Loew LM, Ohrbach HS, Catrelli M, Cohen LB Cell Structure and Function by Microfluorometry 1989 Jan;329-346
  • Nernstian dye distribution reports membrane potential in individual cells
    Ehrenberg B, Wei M.-d., Loew LM Membrane Proteins 1987 Jan;279-294

Conference Papers

Editorials

Letters

Reviews

Short Surveys

Title or AbstractTypeSponsor/EventDate/YearLocation
Multiscale and Multiphysics Modeling within the Virtual Cell EnvironmentLectureInteragency Modeling and Analysis Group2014Bethesda, MD
Taming the Complexity of Cell Biology with Virtual CellLectureUniversity of Kansas2014Lawrence, KS
How to get your paper publishedPanel DiscussionBiophysical Society2014San Francisco, CA
Virtual Cell ProjectLectureNIMBios Workshop2013Knoxville, TN
Computational Cell Biology CourseLectureCold Spring Harbor Lab2013Long Island, NY
Optical Electrophysiology with Voltage Sensitive DyesLectureBruce Rappaport Faculty of Medicine Seminar, Technion2013Haifa, Israel
Membrane Potential ImagingPlenary LectureIsrael society for Microscopy2013Tsfat, Israel
The Virtual Cell ProjectLectureWeizmann Institute of Science2013Rehovot, Israel
Taming the complexity of cellular biology with Virtual CellLectureBar Ilan University2013Ramat Gan, Israel
Multiscale and Multiphysics Modeling with Virtual CellLectureWorkshop on Spatial Modeling, Carnegie Mellon U.2013Pittsburgh, PA
Multiscale and Multiphysics Modeling with Virtual CellLectureMultiscale Neuronal Modeling Workshop, U. California San Diego2013San Diego, CA
The Virtual Cell ProjectLectureChemistry Dept. Seminar, City College of CUNY2013New York, NY
The Virtual Cell ProjectLectureBiomathematics Seminar2012Ohio State University, Columbus OH
Neuronal circuits - From molecules to oscillationsTalkII Simpósio da PPG-Neuro2012Pipa, Brazil
Biophysics Seminar SeriesTalkMIT2012Cambridge, MA
Computational Cell Biology CourseTalkCold Spring Harbor Lab2012Long Island, NY
Workshop on “How to get your paper published”TalkBiophysical Society2012San Diego, CA
Biophysics Seminar SeriesTalkBar Ilan University2012Ramat Gan, Israel
The virtual CellLecture3D Virtual Cell Project2012San Diego, CA
Computational Cell Biology CourseTalkCold Spring Harbor Lab2011Long Island, NY
Workshop on "single cell" analysisTalkNIH2011Bethesda, MD
Neuromechanics WorkshopTalkFields Institute2010Toronto, Ont., Canada
Biomedical Engineering Graduate Student Committee Research SeminarTalkUniversity of Connecticut Health Center2010Farmington, CT
Computational Cell Biology SymposiumTalkWellcome Trust Genome Campus2010UK
National Biomedical Computing Center SeminarTalkUniversity of California2010San Diego, CA
Biomedical Technology Research MeetingTalkNIH2010Rockville, MD
Biology Department Seminar SeriesTalkUniversity of Texas2010San Antonio, TX
Computational Cell Biology CourseTalkCold Spring Harbor Lab2010Long Island, NY
Forces in Biology WorkshopTalkUniversity of North Carolina2009Chapel Hill, NC
Workshop on Cell biology OntologyTalkNIH2009Bethesda, MD
Microscope Imaging SeminarTalkSowers Institute2009Kansas City, KS
Cell Biology SeminarTalkSowers Institute2009Kansas City, KS
Biomedical Engineering SeminarTalkJohn Hopkins University2009Baltimore, MD
Voltage Imaging WorkshopTalkHoward Hughes Foundation2009Janelia Farm, VA
Computational Cell Biology CourseTalkCold Spring Harbor Lab2009Long Island, NY
Systems Biology SeminarTalkUniversity of North Carolina2009Chapel Hill, NC
Neuroengineering WorkshopTalkPenn State2008State College, PA
Neuroscience SeminarTalkPenn State2008State College, PA
Frontiers in Cell MigrationTalkNIH2008Bethesda, MD
Course on Computational Cell BiologyTalkCold Spring Harbor Laboratory2008Long Island, NY
BioelectrochemistryTalkGordon Research Conference 2008Biddeford, ME
Research SeminarTalkBoehringer Ingelheim Pharmaceuticals2008Ridgefield, CT
Vascular Biology and Therapeutics SeminarTalkYale University 2008New Haven, CT
Biophysics ColloquiumTalkBar Ilan Universty2007Ramat Gan, Israel
Cyd Hassner Memorial LectureTalkBar Ilan Universty2007Ramat Gan, Israel
Center for Vascular Biology Retreat KeynoteTalkUniversity of Connecticut Health Center2007Farmington, CT
Workshop on Chemistry in NeuroscienceTalkHHMI Janelia Farm 2007Ashburn, VA
Proteomics ConferenceTalkNIH/NHLBI 2007Washington, DC
Workshop on Biological Physics of Cellular ProcessesTalkPhysics Dept. UCSD2007San Diego, CA
Wesleyan Hughes Seminar SeriesTalkWesleyan University2007Middletown, CT
Michigan Alliance for Cheminformatic ExplorationTalkUniversity of Michigan2007Ann Arbor, MI
Methods in Clinical ResearchTalkUniversity of Connecticut Health Center2007Farmington, CT
Computational Cell Biology MeetingTalkCold Spring Harbor Laboratory2007Long Island, NY
Tri-Institutional Computational and Systems Biology SeminarTalkMemorial Sloan-Kettering2007New York, New York
Biophysics ColloquiumTalkBar Ilan University 2006Ramat Gan, Israel
Biological Networks and Pathways SymposiumTalkJohn Hopkins University2006Baltimore, MD
Interdisciplinary Seminars on Complex NetworksTalkUniversity of Illinois2006Urbana, IL
Calcium and Cell FunctionTalkFASEB Summer Research Conferences2006Snowmass, CO
Macromolecular Organization & Cell FunctionTalkGordon Research Conference2006Holyoke, MA
Infectious Disease Informatics Working GroupTalkNSF2005Arlington, VA
Nanomedicine MeetingTalkNIH2005Gaithersberg, MD
Proteome Interest GroupTalkNIH2005Bethesda, MD
Frontiers of Science ProgramTalkNew York Academy of Sciences2005New York, NY
4th Annual Genomics and Bioinformatics SymposiumTalkYale University 2005New Haven, CT