Ann Cowan, Ph.D.
Professor of Molecular, Microbial and Structural BiologyDeputy Director, Center for Cell Analysis and Modeling
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Academic Office Location: Molecular, Microbial and Structural Biology University of Connecticut Health Center 263 Farmington Avenue Farmington, CT 06030-6406 |
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| Phone: | 860-679-1449 |
| Fax: | 860-679-1039 |
| Email: | acowan@nso2.uchc.edu |
| Website(s): |
Cell Biology Graduate Program Cell Analysis and Modeling Graduate Program Moelcular Biology & Biochemistry Graduate Program |
Education
Post-Graduate Training
Awards
| Degree | Institution | Major |
|---|---|---|
| Ph.D. | University of Colorado | Molecular, Cellular, and Developmental Biology |
| M.S. | Yale University | Biology |
| B.S. | Queen’s University | Biology |
Post-Graduate Training
| Training | Institution | Specialty |
|---|---|---|
| Professional Training | NIH | National Institutes of Health Predoctoral Traineeship |
| Fellowship | Lalor Foundation | Postdoctoral Fellowship |
| Post Doctoral | Damon Runyon-Walter Winchell Cancer Fund | |
| Post Doctoral | University of Connecticut Health Center | Department of Physiology |
Awards
| Name of Award/Honor | Awarding Organization |
|---|---|
| Osborn Biomedical Science Graduate Teaching Award Recipient | Graduate Student Organization |
| Postdoctoral Fellowship | Lalor Foundation |
| Postdoctoral Fellowship 1984-1987 | Damon Runyon-Walter Winchell Cancer Fund |
| B. Sc. (Honours) with First Class Honours | Queen’s University |
| Predoctoral Traineeship 1977-1980 | National Institute of Health |
My general research interests center on understanding membrane organization, particularly the organization of structural and functional domains within the plasma membrane. A primary research project explores the development and maintenance of plasma membrane domains during mammalian spermatogenesis using biochemical techniques, high resolution light microscopy, and, in collaboration with Dr. Dennis Koppel, biophysical analyses such as fluorescence photobleaching. A second project involves an ongoing collaboration with Dr. Peter Setlow in the department to analyze the dramatic changes in membrane organization that occur during sporulation in Bacillus subtilis.
As Deputy Director of the Center for Cell Analysis and Modeling, I oversee our light microscopy user facility, which provides access to state-of-the-art instrumentation in high-resolution fluorescence microscopy to the general research community. The Center for Biomedical Imaging Technology is also home to the NIH-funded National Resource for Cell Analysis and Modeling (NRCAM). NRCAM develops tools for quantitative cell biology research. Our approach is to combine high resolution quantitative imaging and biochemical analyses with mathematical modeling to develop computational simulations of specific cellular events. NRCAM has created the “Virtual Cell” modeling environment, a tool for creating spatially realistic mathematical models of cellular processes using a web-based interface that is freely available to the academic research community.
As Deputy Director of the Center for Cell Analysis and Modeling, I oversee our light microscopy user facility, which provides access to state-of-the-art instrumentation in high-resolution fluorescence microscopy to the general research community. The Center for Biomedical Imaging Technology is also home to the NIH-funded National Resource for Cell Analysis and Modeling (NRCAM). NRCAM develops tools for quantitative cell biology research. Our approach is to combine high resolution quantitative imaging and biochemical analyses with mathematical modeling to develop computational simulations of specific cellular events. NRCAM has created the “Virtual Cell” modeling environment, a tool for creating spatially realistic mathematical models of cellular processes using a web-based interface that is freely available to the academic research community.
Not accepting students for Lab Rotations at this time
Journal Articles
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Expression level of Bacillus subtilis germinant receptors determines the average rate but not the heterogeneity of spore germination
(2013) J Bacteriol 8 (195) 1735-40
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Novel 3D analysis of Claudin-5 reveals significant endothelial heterogeneity among CNS microvessels
(2013) Microvasc Res (86) 10-Jan
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Novel 3D analysis of Claudin-5 reveals significant endothelial heterogeneity among CNS microvessels
(2012) Microvasc Res
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Oligomer size of the serotonin 5-hydroxytryptamine 2C (5-HT 2C) receptor revealed by fluorescence correlation spectroscopy with photon counting histogram analysis: Evidence for homodimers without monomers or tetramers
(2012) Journal of Biological Chemistry 28 (287) 23604-23614
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Spatial modeling of cell signaling networks
(2012) Methods Cell Biol (110) 195-221
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Germination proteins in the inner membrane of dormant Bacillus subtilis spores colocalize in a discrete cluster
(2011) Mol Microbiol 4 (81) 1061-1077
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Vacuolar Protein Sorting Protein 13A, TtVPS13A, Localizes to the Tetrahymena thermophila Phagosome Membrane and Is Required for Efficient Phagocytosis
(2011) Eukaryot Cell 9 (10) 1207-1218
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Dynamics of peptidergic secretory granule transport are regulated by neuronal stimulation
(2010) BMC Neuroscience (11) 32
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Analysis of dye binding by and membrane potential in spores of Bacillus species
(2009) Journal of Applied Microbiology 3 (106) 814-824
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Levels and localization of mechanosensitive channel proteins in Bacillus subtilis
(2009) Archives of Microbiology 5 (191) 403-414
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Role for Rab3a in oligodendrocyte morphological differentiation
(2009) Journal of Neuroscience Research 2 (87) 342-352
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Using the virtual cell simulation environment for extracting quantitative parameters from live cell fluorescence imaging data
(2009) Microscopy and Microanalysis SUPPL. 2 (15) 1522-1523
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Virus-Induced Chaperone-Enriched (VICE) domains function as nuclear protein quality control centers during HSV-1 infection.
(2009) PLoS pathogens 10 (5)
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Characterization of spores of Bacillus subtilis that lack most coat layers
(2008) Journal of Bacteriology 20 (190) 6741-6748
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Cyclic 3',5'-AMP causes ADAM1/ADAM2 to rapidly diffuse within the plasma membrane of guinea pig sperm
(2008) Biology of Reproduction 5 (79) 999-1007
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Luteinizing hormone causes MAP kinase-dependent phosphorylation and closure of connexin 43 gap junctions in mouse ovarian follicles: One of two paths to meiotic resumption
(2008) Development 19 (135) 3229-3238
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Essential role for prolyl hydroxylase domain protein 2 in oxygen homeostasis of the adult vascular system
(2007) Circulation 7 (116) 774-781
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How moist heat kills spores of Bacillus subtilis
(2007) Journal of Bacteriology 23 (189) 8458-8466
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Membrane Hemifusion Is a Stable Intermediate of Exocytosis
(2007) Developmental Cell 4 (12) 653-659
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Microtubule motor Ncd induces sliding of microtubules in vivo
(2007) Molecular Biology of the Cell 9 (18) 3601-3606
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Planar spindle orientation and asymmetric cytokinesis in the mouse small intestine
(2007) Journal of Histochemistry and Cytochemistry 11 (55) 1173-1180
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A role for Sec8 in oligodendrocyte morphological differentiation
(2006) Journal of Cell Science 5 (119) 807-818
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FUS1 regulates the opening and expansion of fusion pores between mating yeast
(2006) Molecular Biology of the Cell 5 (17) 2439-2450
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Pixel intensity and fractal analyses: detecting osteoporosis in perimenopausal and postmenopausal women by using digital panoramic images
(2006) Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology and Endodontology 2 (102) 235-241
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Systematic characterization of nuclear proteome during apoptosis
(2006) Molecular and Cellular Proteomics 6 (5) 1131-1145
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Lipids in the inner membrane of dormant spores of Bacillus species are largely immobile
(2004) Proceedings of the National Academy of Sciences of the United States of America 20 (101) 7733-7738
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Sphingosine 1-phosphate receptor regulation of N-cadherin mediates vascular stabilization
(2004) Genes and Development 19 (18) 2392-2403
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The low-density lipoprotein receptor is regulated by estrogen and forms a functional complex with the estrogen-regulated protein ezrin in pituitary GH3 somatolactotropes
(2004) Endocrinology 7 (145) 3075-3083
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A novel engulfment ligand from apoptotic cells identified by quantitative proteomics
(2003) Developmental Cell 4 (4) 587-598
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A soluble protein is immobile in dormant spores of Bacillus subtilis but is mobile in germinated spores: Implications for spore dormancy
(2003) Proceedings of the National Academy of Sciences of the United States of America 7 (100) 4209-4214
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Annexin I is an endogenous ligand that mediates apoptotic cell engulfment.
(2003) Developmental Cell 4 (4) 587-598
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Germination of spores of Bacillus subtilis with dodecylamine
(2003) Journal of Applied Microbiology 3 (95) 637-648
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Tissue-Specific Regulation by Estrogen of Ezrin and Ezrin/Radixin/Moesin- Binding Protein 50
(2003) Endocrine 2 (22) 119-126
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Mechanisms of killing spores of Bacillus subtilis by acid, alkali and ethanol
(2002) Journal of Applied Microbiology 2 (92) 362-375
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Reciprocal regulation by estradiol 17-beta of ezrin and cadherin-catenin complexes in pituitary GH3 cells
(2002) Endocrine 3 (17) 219-228
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Studies on the mechanism of killing of Bacillus subtilis spores by hydrogen peroxide
(2002) Journal of Applied Microbiology 2 (93) 316-325
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Guinea pig fertilin exhibits restricted lateral mobility in epididymal sperm and becomes freely diffusing during capacitation
(2001) Developmental Biology 2 (236) 502-509
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Analysis of nucleoid morphology during germination and outgrowth of spores of Bacillus species
(2000) Journal of Bacteriology 19 (182) 5556-5562
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Mutagenesis studies of human red opsin: trp-281 is essential for proper folding and protein-retinal interactions
(1998) Biochemistry 50 (37) 17487-17494
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Barriers to diffusion of plasma membrane proteins form early during guinea pig spermiogenesis
(1997) Biophysical Journal 1 (73) 507-516
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Analysis of the relationship between the decrease in pH and accumulation of 3-phosphoglyceric acid in developing forespores of Bacillus species
(1996) Journal of Bacteriology 8 (178) 2204-2210
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Surface expression of the pre-beta subunit of fertilin is regulated at a post-translational level in guinea pig spermatids
(1995) Developmental Biology 2 (168) 429-437
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Scanning concentration correlation spectroscopy using the confocal laser microscope
(1994) Biophysical Journal 2 I (66) 502-507
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The internal pH of the forespore compartment of Bacillus megaterium decreases by about 1 pH unit during sporulation
(1994) Journal of Bacteriology 8 (176) 2252-2258
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Biogenesis of surface domains during spermiogenesis in the guinea pig
(1993) Developmental Biology 1 (155) 124-133
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Migration of the guinea pig sperm membrane protein PH-20 from one localized surface domain to another does not occur by a simple diffusion-trapping mechanism
(1991) Developmental Biology 1 (144) 189-198
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Fully effective contraception in male and female guinea pigs immunized with the sperm protein PH-20
(1988) Nature 6190 (335) 543-546
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Purification of the guinea pig sperm PH-20 antigen and detection of a site-specific endoproteolytic activity in sperm preparations that cleaves PH-20 into two disulfide-linked fragments
(1988) Biology of Reproduction 4 (38) 921-934
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Lateral diffusion of the PH-20 protein on guinea pig sperm: Evidence that barriers to diffusion maintain plasma membrane domains in mammalian sperm
(1987) Journal of Cell Biology 4 (104) 917-923
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Rearrangement of sperm surface antigens prior to fertilization
(1987) Annals of the New York Academy of Sciences (513) 262-273
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Sperm exocytosis increases the amount of PH-20 antigen on the surface of guinea pig sperm
(1986) Journal of Cell Biology 4 (103) 1289-1297
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Mapping the distribution of differentiation potential for intestine, muscle, and hypodermis during early development in Caenorhabditis elegans.
(1985) Cell 3 (41) 923-932
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The formation of several mitochondrial enzymes during the cell cycle in heat-synchronized Tetrahymena pyriformis ST.
(1978) Experimental Cell Research 1 (112) 78-87
Conference Papers
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Lanthanide Enhanced Luminescence (LEL) with one and two photon excitation of Quantum Dyes® Lanthanide(III)-Macrocycles
(2004) Proceedings of SPIE - The International Society for Optical Engineering (5322) 187-199
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Computational cell biologists snowed in at Cranwell
(2001) Trends in Cell Biology 6 (11) 236-238
| Title or Abstract | Type | Sponsor/Event | Date/Year | Location |
|---|---|---|---|---|
| Talk Unknown | Talk | National Technology Centers for Networks and Pathways All Hands Meeting | 2007 | Bethesda, MD |
| Booth at 2006 Annual Meeting Society for the Advancement of Chicano and Native American Scientists for student recruitment | Other | 2006 Annual Meeting Society for the Advancement of Chicano and Native American Scientists | 2006 | |
| Title Unknown | Talk | Howard University Workshop on Computational Cell Biology | 2005 | Washington, CT |
| Title Unknown | Talk | Northeast Spore Meetings, | 2002 | Harvard University, Cambridge, MA |
| Title Unknown | Talk | Center for Microbial Pathogenesis, UCHC | 2002 | |
| Title Unknown (cancelled due to 9/11 events) | Talk | Iowa Microscopy Society Meeting, University of Iowa | 2001 | Ames, IO |
| Confocal Demonstration and Tour for Avon High School students | Other | UConn Health Center | 2001 | Farmington, CT |
| Confocal Demonstration and Tour, UConn Clinical Engineering students | Other | UConn Health Center | 2000 | Farmington, CT |
| Confocal Microscopy Demonstration for students in Biological Structure Course at Wesleyan University | Other | UConn Health Center | 1999 | Middletown, CT |
| Title Unknown | Talk | Society for the Study of Reproduction Annual Meeting | 1998 | College Station, TX |
| Title Unknown | Talk | The Population Council, Center for Biomedical Research | 1997 | New York, NY |
| Photonics: Teaching Across the Spectrum | Talk | Conference for Science and Technology Teachers, University of Connecticut | 1997 | Storrs, CT |
| Confocal Demonstration and Tour, Science Teacher Onsite Visit | Other | Middletown High School | 1997 | Middletown, CT |
| CPTV’s Family Science Expo | Other | CT Public TV | 1997 | Hartford, CT |
| Targets for Contraceptive Development | Talk | NICHD workshop, NIH | 1994 | Bethesda, MD |
| Title Unknown | Talk | Loeb Institute, Civic Hospital, | 1993 | Ottawa, Ontario, Canada |
| Title Unknown | Talk | Department of Anatomy and Cell Biology. University of Alberta | 1992 | Calgary, Alberta, Canada |
| Title Unknown | Talk | Department of Obstetrics and Gynecology, Tufts University School of Medicine | 1992 | Boston, MA |
| title Unknown | Talk | Department of Anatomy, University of Arizona Health Sciences Center | 1991 | Tuscon, AR |
| Fertilization and Activation of Development | Talk | Gordon Conference | 1991 | NH |
| Title Unknown | Talk | Department of Biological Sciences, SUNY at Buffalo | 1990 | Buffalo, NY |
| Title Unknown | Talk | Division of Reproductive Biology, University of Pennsylvania Medical Center | 1989 | PA |