Photo of Justin D. Radolf, M.D.

Justin D. Radolf, M.D.

Professor of Medicine
Academic Office Location:
UConn Health
263 Farmington Avenue
Farmington, CT 06030-3715
Phone: 860-679-8480
Fax: 860-679-8130

Genetics & Developmental Biology Graduate Program

Immunology Graduate Program

Spirochete Research Labs

M.D.University of California San FranciscoMedicine
B.S.Yale University Biology

Post-Graduate Training
ResidencyUCLAInternal Medicine
FellowshipUCLAInfectious Diseases
FellowshipUCLA Postdoctoral Research Fellow

Name of Award/HonorAwarding Organization
Merit Award NIH/NIAID
Established Investigatorship Award, 1992-97American Heart Association
Scholar, 1989-92 American Foundation for AIDS Research
Pfizer Scholars Award for New Faculty 1986-88Pfizer
Young Investigator Award American Society for Microbiology
Pfizer Medical Research Merit Award PFIZER CORPORATION
Postdoctoral Research Fellowship 1984-86 American Social Health Association
National Merit Scholar
Lyme Disease/Borrelia burgdorferi

The Lyme disease spirochete B. urgdorferi is maintained in nature via an enzootic cycle in which it is transmitted by the nymphal stage of its vector, the deer tick Ixodes scapularis, to a mammalian host (typically the white-footed mouse Peromyscus leucopus) and then is acquired when a naïve larval tick feeds on an infected mouse. Infection of humans with B. burgdorferi is accidental and is not required for persistence of the spirochete in nature. In order to transit between its arthropod and mammalian hosts, spirochetes must decipher complex environmental cues delivered at the feeding site and, in response, undergo dramatic changes in their transcriptomes and proteomes. The principal objective of Lyme disease research conducted in the Radolf Laboratory is to understand these processes. The alternative sigma factor RpoS is a unifying genetic feature of this project. Signals delivered by the blood meal induce the expression of RpoS which, as the promoter-reading subunit of RNA polymerase, induces far-reaching changes in the bacterium’s transcriptome: (1) upregulation of approximately 100 B. burgdorferi genes that we believe are required for transmission of spirochetes from tick to mouse and/or the establishment of infection once within the mouse and (2) downregulation of approximately 30 tick-phase genes that spirochetes no longer need once they are inoculated into mice.

Critical to this work has been our development of green fluorescent protein (GFP) reporters that enable us to track live spirochetes in ticks and mice. Our live-imaging studies have fundamentally changed our understanding of the transmission process. In order to reach the mouse, spirochetes disseminate through the midgut into the salivary glands in order to access the salivary stream which they “ride” into the vertebrate host. We have found that dissemination of spirochetes in ticks is actually biphasic. In the first phase, which we have termed “adherence-mediated migration, spirochetes replicate in close association with differentiating midgut epithelial cells, “working” their way as aggregates or networks to the base of the epithelium. In the second phase, they transition into typically motile spirochetes, complete the penetration through the midgut, and then move on to the salivary glands en route to the mouse. Most recently, we have found that spirochetes lacking RpoS are deficient in this process and we are developing various strategies to identify the RpoS-dependent genes involved.

Syphilis/Treponema pallidum

The syphilis spirochete T. pallidum harbors many resemblances to B. burgdorferi but actually employs a markedly different parasitic strategy. Whereas B. burgdorferi is an enzootic pathogen, T. pallidum is an obligate pathogen of humans which cannot be cultivated in artificial medium. The modes of transmission of the two bacteria differ markedly as well: T. pallidum is transmitted from person-to-person by sexual activity, whereas B. burgdorferi is transmitted by an arthropod vector. Once within the host, T. pallidum begins to replicate locally, eventually causing a genital ulcer, called a chancre, the clinical hallmark of the primary stage of the disease. As the chancre develops, treponemes begin to make their way towards draining lymph nodes and blood vessels in order to spread systemically. Once within the blood, T. pallidum is extremely adept at invading virtually every organ system in the body, including the central nervous system, and establishing persistent infection that can cause serious, even life threatening, complications months to years later. We have designated T. pallidum “the stealth pathogen” because of its remarkable ability to evade host immune defenses.

Efforts in the Radolf Laboratory to explain T. pallidum’s stealth pathogenicity have focused on the bacterium’s unusual molecular architecture. Over the years, we have generated abundant evidence that the T. pallidum outer membrane differs markedly in structure and composition from its gram-negative counterparts (e.g., Escherichia coi). Not only does it lack lipopolysaccharide, the highly inflammatory glycolipid in the outer membranes of gram negative bacteria, it also contains a much lower density of integral membrane proteins that present few surface antigenic targets to the host immune system. Situated below the outer bilayer, where they are inaccessible to antibodies in intact organisms, are the bacterium’s major immunogens, many of which are periplasmic proteins tethered by N-terminal lipids to the cytoplasmic membrane. This work ushered in what we have termed “the quest” for T. pallidum outer membrane proteins, a project that has been ongoing for more than 20 years. Why a quest? Because identifying rare outer membrane proteins is so difficult and requires extraordinary commitment. Fortunately, we now have much more powerful tools to fulfill the quest, among which is the complete genomic sequence of T. pallidum. Genome mining, however, isn’t as easy as it sounds because, with one exception, there are no proteins in the T. pallidum genome with sequence relatedness to well characterized outer membrane proteins of gram-negatives. This work is complicated further by the fragility of the treponeme’s outer membrane. Our genereal strategy is to use bioinformatics algorithms to identify outer membrane protein candidates that then must be cloned, expressed, purified, structurally characterized, and localized in live treponemes. Why is this quest important? Two reasons. First, outer membrane proteins provide channels through which bacteria obtain nutrients. Second, we believe that these surface-exposed proteins, few as they are, are likely vaccine candidates. Given the explosive increase in syphilis cases in the United States and the world during the past decade, a vaccine would be a major weapon in our battle against this centuries old affliction of humans.

In order to fulfill its genetic destiny as a stealth pathogen, T. pallidum must acquire nutrients in every milieu within its obligate human host in which it finds itself, while fending off the host’s attempts to undermine its homeostasis. Recognition of this metabolic war between pathogen and host led us to explore other facets of T. pallidum virulence. One has been transition metal acquisition. Transition metals, such as iron, manganese, and zinc, are essential for life but are present in mammalian body fluids at exceedingly low concentrations. Bacterial pathogens, T. pallidum being no exception, employ highly developed strategems to obtain these nutrients. Our work along these lines has centered about characterizing two ABC transporters within the cytoplasmic membrane (Tro and Znu) that work cooperatively to meet the bacterium’s metal requirements. Lastly, our immune system uses toxic compounds called reactive oxygen species, to kill bacteria. T. pallidum has extremely robust enzymatic mechanisms for detoxifying reactive oxygen species. Understanding how these enzymes work and are regulated in response to host defenses is relevant to all bacterial diseases, not just syphilis.

Accepting Lab Rotation Students: Summer '15, Fall '15, Spring '16

Lab Rotation Projects

Molecular Pathogenesis of Syphilis and Lyme Disease

1. Differential gene expression by Borrelia burgdorferi, the Lyme disease spirochete. B. burgdorferi undergoes dramatic changes in gene expression and protein composition as it cycles back and forth between its arthropod vector (deer ticks) and mammalian host (mice and other rodents). We have developed a variety of genetic techniques that, in conjunction with microarray analysis, are enabling us to decipher the patterns of borrelial gene expression at various points in the enzootic cycle, the mechanisms that regulate differential gene expression, and the relevant signal transduction mechanisms.

2. Outer membrane architecture of Treponema pallidum, the syphilis spirochete. We demonstrated a number of years ago that the T. pallidum outer membrane differs markedly in ultrastructure and composition from the “conventional” outer membranes of gram-negative bacteria, such as E. coli. We have identified a novel T. pallidum outer membrane protein that integrates into the outer membrane lipid bilayer via covalently bound lipids and amphipathic helices. This protein also appears to promote solute uptake by destabilizing the lipid bilayer, creating pores. Using model membrane systems, we would like to understand more about the structure-function relationships of this unusual molecule.

Journal Articles

Book Chapters

  • Borrelia: Molecular Biology, Host Interaction and Pathogenesis
    Radolf JD, Salazar JC, and Dattwyler RW. Lyme disease in humans (Chapter 18). 2009 Jan;487-533
  • Manual of Clinical Microbiology, (Chapter 57)
    Radolf JD, Pillay A, and Cox DL. Treponema and Brachyspira, human host-associated spirochetes. 2009 Jan;
  • Sexually Transmitted Diseases
    Shafii, T., Radolf JD, Sánchez PJ, Schulz KF, and Murphy FK. Congenital Syphilis (Chapter 82) 2008 Jan;
  • Chapter 10 - Treponema: Molecular and Cellular Biology
    Radolf JD, Hazlett KR, and Lukehart SA. Pathogenesis of Syphilis. Chapter 10. 2006 Jan;
  • Chapter 13 - Treponema: Molecular and Cellular Biology
    Radolf JD and Lukehart SA. Immunology of Syphilis. Chapter 13. 2006 Jan;
  • Chapter 4 - Treponema: Molecular and Cellular Biology
    Cox DL and Radolf JD. Metabolism of the Treponema,Chapter 4. 2006 Jan;
  • Molecular Biology of Spirochetes.
    Eggers CH, Caimano MJ, and Radolf JD. Use of green fluorescent protein transcriptional reporters to study differential gene expression by Borrelia burgdorferferi. 2006 Jan;
  • Infectious Diseases
    Salazar JC and Radolf JD. Management of an HIV-positive pregnant woman with a positive VDRL test from an area endemic for Treponema infection 2002 Jan;
  • The Prokaryotes: An Evolving Electronic Resource for the Microbiological Community
    Caimano MJ and Radolf JD. The Genus Borrelia. 2002 Jan;
  • Infectious Diseases
    Nassar NN and Radolf JD. Management of a positive VDRL in HIV-infected pregnant women from areas endemic for non-venereal treponematoses (Chapter 56). 1999 Jan;
  • Sexually Transmitted Diseases
    Radolf JD, Sánchez PJ, Schulz KF, Murphy FK. Congenital Syphilis (Chapter 84) 1999 Jan;
  • Medical Microbiology
    Radolf JD. Treponema (Chapter 36) 1996 Jan;
  • Atlas of Infectious Diseases. Volume II. GE Mandell (Editor-in-Chief) and DL Stevens (Editor). Churchill Livingstone, Philadelphia, PA.
    Chiu MJ, Cockerell CJ, Haupt KR, and Radolf JD. Spirochetal Infections of the Skin. (Chapter 12). 1995 Jan;
  • Opportunistic Complications of HIV (Sexually Transmitted Diseases)
    Radolf JD. Syphilis in the setting of HIV disease: opportunistic infection or fellow traveler? 1995 Jan;13-24
  • Conn's Current Therapy.
    Radolf JD. Syphilis. 1994 Jan;699-702
  • Infectious Diseases: A Treatise of Infectious Processes.
    Chiu MJ and Radolf JD. Syphilis. Chapter 73. 1994 Jan;694-714
  • Diagnostic Molecular Microbiology: Principles and Applications.
    Radolf JD. PCR Detection of Treponema pallidum 1993 Jan;
  • Lyme Disease: Molecular and Immunologic Approaches
    Radolf JD, Brusca JS, Brandt ME, Norgard MV. Spirochete Molecular Architecture and Lyme Disease Pathogenesis. 1992 Jan;119-134
  • Textbook of Internal Medicine.
    Isaacs RD, Radolf JD. Syphilis (Chapter 291). 1991 Jan;

Conference Papers





  • Expert Review on: Biological false-positive tests comprise a high proportion of Venereal Disease Research Laboratory reactions in an analysis of 300,000 sera. Geusau A, Kittler H, Hein U, Dangl-Erlach E, Stingl G, Tschachler E.
    Radolf JD. Int J STD AIDS 2005 Jan;16722-726
  • Review of “Bull’s Eye: Unraveling the Medical Mystery of Lyme Disease”
    Radolf, J. Jonathan A. Edlow J Clin Invest 2004 Jan;1131378
  • The immune response to infection with Treponema pallidum, the stealth pathogen.
    Salazar, Juan C; Hazlett, Karsten R O; Radolf, Justin D Microbes and infection / Institut Pasteur 2002 Sep;4(11):1133-40
  • Borrelia burgdorferi gene expression profiling with membrane-based arrays.
    Ojaimi, Caroline; Brooks, Chad; Akins, Darrin; Casjens, Sherwood; Rosa, Patricia; Elias, Abdallah; Barbour, Alan; Jasinskas, Algis; Benach, Jorge; Katonah, Laura; Radolf, Justin; Caimano, Melissa; Skare, Jon; Swingle, Kristen; Sims, Simon; Schwartz, Ira Methods in enzymology 2002 Jan;358165-77
  • Treponema pallidum: doing a remarkable job with what it’s got
    Radolf JD, Steiner B, and Shevchenko D. Trends Microbiol 1999 Jan;77-9
  • Molecular approaches to improved syphilis serodiagnosis.
    Isaacs RD and Radolf JD. Serodiag. Immunother Infect Dis 1989 Jan;3299-306
Title or AbstractTypeSponsor/EventDate/YearLocation
The going and coming of Lyme disease: differential gene expression, environmental sensing, and spirochete trackingTalkDepartment of Microbiology and Immunology2011University of Calgary, Calgary, CA.
The going and coming of Lyme disease: differential gene expression, environmental sensing, and spirochete trackingTalkInter-city Infectious Diseases Conference2011University of Connecticut Health Center.
Tracking spirochetes and spirochete gene expression: illuminating the pathogenesis of Lyme diseaseTalkDepartment of Microbiology, LSU Veterinary School2010Baton Rouge, LA
Molecular architecture of Treponema pallidum: making a living as a stealth pathogenTalkDepartment of Pathology and Laboratory Medicine2010UT Houston Medical Center.
Tracking spirochetes and spirochete gene expression: illuminating the pathogenesis of Lyme diseaseTalkDepartment of Microbiology and Immunology2009University of Kentucky, Lexington, KY
Immune recognition of Borrelia burgdorferi, the Lyme disease spirochete by phagosomal signaling: signaling from the graveTalkDepartment of Microbiology and Immunology2009U. of MD School of Medicine, Baltimore, MD.
Tracking spirochetes and spirochete gene expression: illuminating the pathogenesis of Lyme diseaseTalkDepartment of Microbiology and Molecular Genetics2009Stony Brook University, Stony Brook, NY
Tracking spirochetes and spirochete gene expression: illuminating the pathogenesis of Lyme diseaseTalkDepartment of Oral Biology, School of Dental Medicine2009SUNY at Buffalo, Buffalo, NY
The Quest for Treponema pallidum outer membrane proteins: a reappraisalTalkEighth Gordon Research Conference on the Biology of Spirochetes2008Ventura, CA.
Immune recognition of pathogenic spirochetes by phagosomal signaling TalkDepartment of Immunology2008UCHC, Farmington, CT.
Immune recognition of pathogenic spirochetes by phagosomal signalingTalkForsyth Institute2008Harvard School of Dental Medicine, Boston, MA.
Summary/Highlights: Basic Science Track Talk17th meeting of the ISSTDR/10th IUSTI World Congress2007Seattle, WA
Treponema pallidum, the syphilis spirochete: making a living as a stealth pathogenTalkDepartment of Microbiology & Immunology Research Seminar2007Med. Coll. VA., Richmond, VA.
Treponema pallidum, the syphilis spirochete: making a living as a stealth pathogenTalkDepartment of Microbiology & Immunology Research Seminar2007Virginia Commonwealth University, Richmond, VA
Spirochetes and spirochetal diseasesTalkMedical College of Virginia 2007Virginia Commonwealth University, Richmond, VA.
Outer membrane proteins of Treponema pallidum, the syphilis spirochete TalkHauptman-Woodward Institute2007Buffalo, NY
Recognition of pathogenic spirochetes by phagosomal signalingTalkDepartment of Veterinary and Animal Sciences2007University of Massachusetts, Amherst, MA.
Microbial Pathogenesis: a new frontier in translational researchTalkConnecticut Children’s Medical Center2007Hartford, CT
Syphilis Pathogenesis: a post-genomics perspective TalkInfectious Diseases Grand Rounds2007New York Medical College, Valhalla, NY.
The Treponema pallidum outer membrane and what lies beneathTalkSymposium on Pathogenic Spirochetes2006UCLA Dept. Micro., Immunology, and Molecular Gen.
Immune recognition by pathogenic spirochetes: looking below the surface TalkDepartment of Medicine Research Seminar 2006U. of Mass.Medical School, Worcester, MA.
Syphilis and Syphilis Research Sponsored by Drs. Socrates Herrara and Miriam Arevalo, Instituto de Inmunologia del Valle. Talkstudents and faculty at Universidad del Valle2005Cali Colombia
Innate and Adaptive Immunity: An Infectious Disease Doc’s Perspective Sponsored by Corporacion de Lucha Contra El SIDA, Cali Colombia. TalkX Curso Internacional de Enfermedades Infecciosas2005Cali Colombia
Innate and Adaptive Immunity in Syphilis and Lyme Disease (Dr. Carol Wu, organizer)TalkUCHC Faculty Research Seminar Series 2005UCHC
Syphilis Research in the 21st Century: One Investigator’s PerspectiveTalk2005Walter Reed Army Inst. for Res., Rockville, MD.
Syphilis Research in the 21st Century: One Investigator’s PerspectiveTalkCIDEIM2005Cali, Colombia.
Infectious Diseases in the Post-Genomics Era Sponsored by Corporacion de Lucha Contra El SIDATalkXI Seminario Integral del Sida y2005Cali Colombia
Use of green fluorescent protein transcriptional reporters to study differential gene expression by Borrelia burgdorferiTalkNATO Advanced Research Workshop on the Molecular Biology of Spirochetes2005Prague, Czech Republic
The Innate-Adaptive Immune Interface in Syphilis and Lyme diseaseTalk2004Albany Medical College, Albany, NY.
Innate and adaptive immunity: an introductionTalkCIDEIM2004Cali, Colombia
Syphilis-HIV Interactions: What we do and don’t know TalkIX Curso Internacional de Enfermedades Infecciosas2004X Seminario Integral del Sida y
Differential gene expression by Borrelia burgdorferi: new tools and new paradigms TalkDepartment of Microbiology and Molecular Genetics2003University of Dentistry and Medicine, New Jersey
20 years of syphilis researchTalkCentro Internacional de Entrenamiento e Investigaciones Medicas (CIDIEM), 2003Cali, Colombia.
Chaired a session on host-pathogen interactions and presented an overview entitled “Lipoproteins, innate immunity, and the cutaneous immune response to Borrelia burgdorferi” TalkFifth Gordon Research Conference on the Biology of Spirochetes2002Ventura, CA.
Differential expression by Borrelia burgdorferi and shuttle vector development: what goes around, comes around TalkWadsworth Public Health Laboratories2002Albany, NY
Organized and convened a colloquium entitled Arthropod-vector interactions: the flip-side of human diseaseTalk102nd General Meeting of the American Society for Microbiology2002Salt Lake City, Utah.
Molecular pathogenesis of Lyme diseaseTalkNinth International Conference on Lyme Borreliosis and other Tick-borne Diseases2002New York, NY.
The roll of toll-like receptors in innate inflammatory and immune responses to spirochetal infections TalkXXVIIth meeting of the Brazilian Society of Immunology2002Salvador, Brazil
Spirochetal lipoproteins as proinflammatory mediators in syphilis and Lyme diseaseTalkDental Dean’s Seminar2001University of Connecticut Health Center.
TLR2 responses in vivo: bridging innate and adaptive immunity (Dr. D. Golenbock, organizer). TalkToll-receptor club2001Boston University Medical Center
Spirochetal lipoproteins as proinflammatory agonists in syphilis and Lyme diseaseTalkDivision of Rheumatology2001New England Medical Center, Boston, MA.
B. burgdorferi lipoproteins and innate immunity in Lyme disease TalkNIH-sponsored workshop on neuroborreliosis2001Airlie Center, Virginia
Development of a Borrelia burgdorferi shuttle vector: turning the tables TalkDepartment of Molecular and Cellular Biology2001University of Connecticut, Storrs
Spirochetal lipoproteins as proinflammatory agonists in syphilis and Lyme disease TalkRheumatology Grand Rounds2000Yale University School of Medicine, New Haven, CT.
Structure-function relationships of Treponema pallidum membrane proteins TalkFourth Gordon Research Conference on the Biology of Spirochetes2000Ventura, California
Treponema pallidum and Syphilis Research: lessons from the genome TalkUCHC GMB&B Faculty Research Seminar Series2000
Treponema pallidum and syphilis research: Lessons from the genomeTalkDepartment of Microbiology2000Indiana U. School of Medicine, Indianapolis, IN.
Spirochetal lipoproteins as proinflammatory agonists in syphilis and Lyme diseaseTalkDivision of Infectious Diseases2000Indiana U. School of Medicine, Indianapolis, IN.
Molecular architecture of Treponema pallidum: implications for syphilis pathogenesis. Keynote address. Talkannual meeting of the Center for Excellence in Vaccine Research at UConn2000Storrs, CT
Molecular architecture of Treponema pallidum: implications for syphilis pathogenesisTalkDepts. of Biochemistry and Microbiology 1999West Virginia U., Morgantown, W.Va.
Spirochetal lipoproteins as proinflammatory agonists in syphilis and Lyme disease TalkInfectious Diseases Division1999Boston University School of Medicine
Molecular architecture of Treponema pallidum: implications for syphilis pathogenesis TalkOswaldo Cruz Foundation1999Salvador, Brazil.
Syphilis Pathogenesis and New Developments in Syphilis DiagnosisTalkBrazilian Congress of Microbiology1999Salvador, Brazil
Molecular architecture of Borrelia burgdorferi: implications for Lyme Disease pathogenesisTalkDept. of Microbiology and Internal Medicine1998UCHC, Farmington, CT.
Molecular architecture of Borrelia burgdorferi: implications for Lyme Disease pathogenesis TalkDept. of Biology and Dept. of Internal Medicine1998Brookhaven Nat. Lab. and SUNY at Stony Brook, NY.
Treponema pallidum biology: doing the best it can with what it’s got and Pathogenesis of syphilis (also served as co-organizer and co-Chair). TalkInternational Business Conference-sponsored symposium on Spirochete Diseases 1998
New model systems for studying mammalian host adaptation by Borrelia burgdorferiTalkseminar to the Depts. of Molecular Biology and Entomology1998Oklahoma State University, Stillwater, OK.
Identification of rare outer membrane proteins in isolated Treponema pallidum outer membranes - a reappraisal TalkThird Conference on the Biology of Spirochetes1998Ventura, CA
Syphilis PathogenesisTalkgraduate student course on bacterial pathogenesis1998NYU Skirball Institute
New model systems for studying differential gene expression by Borrelia burgdorferi Talk1998Skirball Institute, NYU School of Medicine, NYC.
Treponema pallidum molecular architecture: implications for syphilis pathogenesis and A new model system for studying differential gene expression by Borrelia burgdorferiTalkDept. of Microbiology and Immunology1998Medical College of Virginia.
Spirochetal Lipoproteins: Structure-function relationships and host responses Presented as part of a symposium on "Membrane-anchored Molecules" TalkAnnual Meeting of the Society for General Microbiology1997Reading, UK.
Spirochetal lipoproteins as inflammatory mediators in syphilis and Lyme disease TalkDivision of Infectious Diseases/Dept. of Microbiology & Immunology seminar 1997University of North Carolina, Chapel Hill
A new animal model for studying Borrelia burgdorferi in the mammalian host-adapted stateTalkDepartment of Biochemistry & Molecular Biology1997New York Medical College, Valhalla, NY.
A vaccine for syphilis: the never ending story Presented as part of a symposium entitled "Vaccines for Sexually Transmitted Diseases"Talk37th Interscience Conference on Antimicrobial Agents and Chemotherapy (ICAAC), 1997Toronto, CA
Spirochetal lipoproteins as inflammatory mediators in syphilis and Lyme disease TalkDivision of Infectious Diseases/Department of Microbiology seminar 1997LA State U. School of Medicine, New Orleans, LA.
Outer membrane ultrastructure as a virulence determinant of Treponema pallidum and Borrelia burgdorferi TalkSecond Gordon Conference on the Biology of Spirochetes1996Ventura, CA
Lyme Disease 1996: the clouds are partingTalkMcBee Lectureship1996Baylor Medical Center
Lyme Disease 1996TalkDermatology Grand Rounds1996UT Southwestern and St. Paul's Medical Centers
Treponema pallidum molecular architecture: Implications for disease pathogenesis and vaccine development TalkInfectious Diseases Research Seminar Series1996Univ. of Cincinatti, Cincinatti, OH.
Syphilis and HIV: results of a prospective, multicenter, CDC sponsored study of syphilis and HIV infection Sponsored by the Division of Infectious Diseases, UT Southwestern Medical Center and the Dallas County Public Health Department. TalkSexually Transmitted Diseases Seminar Series1996UT Southwestern Medical Center
Borrelia burgdorferi molecular Architecture and Lyme disease pathogenesis TalkDivision of Infectious Diseases seminar1995SUNY at Downstate Medical Center
Syphilis and rare outer membrane proteins TalkKeystone Symposium entitled "Sexually Transmitted Diseases in the HIV Era"1995Keystone, Colorado
Treponema pallidum molecular architecture: Implications for syphilis pathogenesis Talk1995SUNY at Stony Brook
The Molecular biology of Treponema pallidum: Where next?TalkInternational Society for STD Research1995New Orleans, LA
Outer membrane ultrastructure as a major virulence factor of Treponema pallidum TalkFirst Gordon Conference on the Biology of Spirochetes1994Ventura, CA.
Syphilis in the HIV EraTalkInternational Society for Infectious Diseases1994Prague, Czech Republic
Lyme Disease 1993: of mice and men. Presented at the annual meeting TalkOklahoma Infectious Diseases Society1993Oklahoma
Surface components of Borrelia burgdorferi Sponsored by the Lyme Borreliosis Foundation and the Stamford, CT Dept. of Public Health. Talk1992Stamford, CT
Treponema pallidum uItrastructure: Implications for syphilis pathogenesis. Talkannual meeting of the Texas chapter of the American Society of Microbiology1992San Antonio, TX
New Insights into the molecular architecture of Borrelia burgdorferi Talksymposium on the Immunobiology of Lyme Disease1992Cold Spring Harbor, NY.
Immunology and molecular biology of spirochetal lipoproteinsTalkFirst Cold Spring Harbor Laboratory Symposium on the Immunobiology of Lyme Disease1991Cold Spring Harbor, N.Y.
Ultrastructure and molecular biology of Treponema pallidum: Implications for syphilis pathogenesis TalkAnnual Meeting of the American for Microbiology1991
Triton X-114 phase partitioning of spirochetal membrane proteinsTalk7th International Conference on Partitioning in Aqueous Two-Phase Systems1991New Orleans, LA.
Participant and presented a synopsis of my research on T. pallidum molecular biology and ultrastructure. TalkNIH-sponsored symposium entitled "The Molecular Immunology of Sexually Transmitted Diseases"1991Rocky Mountain National Laboratories, Hamilton, MN
Molecular immunology of spirochetal lipoproteins TalkNIH-sponsored symposium on Lyme disease1991Rocky Mnt. National Lab., Hamilton, MT
Invited participant(unable to attend). TalkMolecular Biology of Spirochetes symposium1991Annecy, France
Spirochete ultrastructure and molecular biology: Implications for pathogenesisTalkDivision of Infectious Diseases seminar1991Vanderbilt University, Nashville, TN.
Moderator Molecular biology of pathogensOtherAnnual Meeting of the American Society for Microbiology1990Anaheim, CA.
Syphilis and HIV Infection and Lyme disease 1990 TalkAnnual meeting of the Texas Infectious Diseases Society1990Bandera, TX.
Developing a molecular biology of syphilis TalkInfectious Diseases seminar1990Brown University, Providence, R.I.
Invited participantTalkNIH symposium on Lyme disease1990Bethesda, MD.
Spirochete lipoproteins: Molecular biology and Implications for disease pathogenesis TalkDepartment of Microbiology1990University of Alabama at Birmingham
Molecular and ultrastructural Analysis of the Treponema pallidum outer membrane presented at a World Health Organization-sponsored symposium TalkThe Biology and the Pathogenicity of Treponemes1989University of Birmingham, Birmingham, UK.
Analysis of the Treponema pallidum outer membrane by freeze-fracture electron microscopy. Presented at a symposium entitled Special Topics in Modern Treponema ResearchTalkAnnual Meeting of the American Society for Microbiology1989New Orleans, LA.
Towards a molecular biology of Treponema pallidumTalkSexually Transmitted Diseases Seminar, Harborview Medical Center1989University of Washington, Seattle, WA.
Molecular biology of neurosyphilis TalkMedical Genetics Rounds1989UT Southwestern Medical Center
Participant, panel discussion on Syphilis and HIV InfectionTalkCenters for Disease Control1988Atlanta, GA.
Syphilis 1987: Goodbye, Colombus. TalkSexually Transmitted Diseases Seminar Series and Dallas County Public Health Department1987U.T. Southwestern Medical Center