Mayu Inaba, M.D., Ph.D.Assistant Professor, Department of Cell Biology
|M.D.||Ehime University School of Medicine||Medicine|
|Ph.D.||Graduate School of Kyushu University||Pathological Medicine|
|Name of Award/Honor||Awarding Organization|
|DeLill Nasser Travel Award for Professional Development in Genetics||Genetics Society of America|
|Travel Award for 52nd Annual Meeting of the American Society for Cell Biology||American Society for Cell Biology|
|Research Fellowship 2003 - 2006||Japan Society for the Promotion of Science for Young Scientists|
Adult tissue stem cells produce highly differentiated but short-lived cells throughout life, contributing to the tissue maintenance and repair. To balance between stem cell self-renewal and differentiation, stem cell often divide asymmetrically to produce two distinct daughters. We investigate the molecular and cellular mechanisms that regulate asymmetric stem cell division using male and female Drosophila germline stem cell systems. We are currently focusing on several specific questions, 1) How the niche signal is the spatially restricted with the emphasis on the novel stem cell specific structure, MT (microtubule based)-nanotubes? 2) How are the physical obstacles (e.g., protein diffusion b?arrier) of dividing stem cells localized, and how does it contribute to precise fate asymmetry? 3) How does the niche monitors stem cell behavior to globally regulate stem cell activities? 4) Mechanism of stem cell clonal expansion. Taking the advantage of small size and simple anatomy of Drosophila gonads, we can conduct whole tissue live imaging to monitor in vivo stem cell behavior. We generate tools to investigate various protein dynamics in asymmetrically dividing stem cells. Our effort will contribute to comprehensively understand how the extrinsic and intrinsic regulations are integrated to ensure the precise cell fate determination.
Accepting Lab Rotation Students: Summer '18, Fall '18, Spring '19
1) Live imaging of germline stem cell division and MT-nanotube formation. Investigate several gene functions comparing mutant vs. wild type stem cells.
2) Mechanism of stem cell clonal expansion. Score the clone domination using heat shock mediated genetic marking of stem cell clone. Compare mutant vs. wild type stem cell clones.
3) Global regulation of total stem cell division. Clonal knockdown of candidate genes in small population of stem cells. Look for the gene which affect division rate of neighboring stem cells.