Faculty Directory › UConn Health

Mark W. Maciejewski, PhD

Assistant Professor, Department of Molecular Biology and Biophysics


Academic Office Location: Molecular Biology and Biophysics UConn Health 263 Farmington Avenue Farmington, CT 06030-3305
Phone:	860-679-1943
Fax:	860-679-3408
Email:	markm@neuron.uchc.edu
Website(s):	Molecular Biology & Biochemistry Graduate Program NMR Structural Biology Facility

Education

Degree	Institution	Major
BS	Illinois State University	Chemistry
PhD	The Ohio State University	Biochemistry

Degree

Institution

Major

Illinois State University

Chemistry

PhD

The Ohio State University

Biochemistry

Awards

Name of Award/Honor	Awarding Organization
Postdoctoral Fellowship Grant 1998-2000	National Institutes of Health
Baxter Undergraduate Research Award	Baxter

Name of Award/Honor

Awarding Organization

Postdoctoral Fellowship Grant 1998-2000

National Institutes of Health

Baxter Undergraduate Research Award

Baxter

As the NMR Facility Manager I primarily utilize nuclear magnetic resonance (NMR) to characterize the three dimensional structure, function and dynamics of proteins from a wide range of biological systems. Research in our laboratory has focused in the past on enzymes involved in DNA repair, specifically the base excision repair pathway. More recently several collaborative projects have been initiated studying proteins involved in bacterial cell division, neurite outgrowth, dynein motor complexes, and others. Much future work involve studying several insecticidal neurotoxins from the African funnel web spider with potential to be used in insect control in collaboration with Dr. Glenn King.

Journal Articles

NUScon: a community-driven platform for quantitative evaluation of nonuniform sampling in NMR
Pustovalova,Y. and Delaglio,F. and Craft,D. L. and Arthanari,H. and Bax,A. and Billeter,M. and Bostock,M. J. and Dashti,H. and Hansen,D. F. and Hyberts,S. G. and Johnson,B. A. and Kazimierczuk,K. and Lu,H. and Maciejewski,M. and Miljenovi\'c,T. M. and Mobli,M. and Nietlispach,D. and Orekhov,V. and Powers,R. and Qu,X. and Robson,S. A. and Rovnyak,D. and Wagner,G. and Ying,J. and Zambrello,M. and Hoch,J. C. and Donoho,D. L. and Schuyler,A. D. Magnetic Resonance 2021 Nov;2843-861
Merging NMR Data and Computation Facilitates Data-Centered Research.
Baskaran, Kumaran; Craft, D Levi; Eghbalnia, Hamid R; Gryk, Michael R; Hoch, Jeffrey C; Maciejewski, Mark W; Schuyler, Adam D; Wedell, Jonathan R; Wilburn, Colin W Frontiers in molecular biosciences 2021 Nov;8817175
A novel phosphoglycerol serine-glycine lipodipeptide of Porphyromonas gingivalis is a TLR2 ligand.
Nichols, Frank C; Clark, Robert B; Maciejewski, Mark W; Provatas, Anthony A; Balsbaugh, Jeremy L; Dewhirst, Floyd E; Smith, Michael B; Rahmlow, Amanda Journal of Lipid Research 2020 Dec;121645-1657
Structural and functional analyses of the N-terminal domain of the A subunit of a Bacillus megaterium spore germinant receptor.
Li, Yunfeng; Jin, Kai; Perez-Valdespino, Abigail; Federkiewicz, Kyle; Davis, Andrew; Maciejewski, Mark W; Setlow, Peter; Hao, Bing Proceedings of the National Academy of Sciences of the United States of America 2019 Jun;116(23):11470-11479
Concomitant disorder and high affinity zinc binding in the hZIP4 intracellular loop.
Bafaro, E M; Maciejewski, M W; Hoch, J C; Dempski, R E Protein science : a publication of the Protein Society 2019 Feb;
Nonuniform sampling in multidimensional NMR for improving spectral sensitivity.
Zambrello, Matthew A; Schuyler, Adam D; Maciejewski, Mark W; Delaglio, Frank; Bezsonova, Irina; Hoch, Jeffrey C Methods (San Diego, Calif.) 2018 Mar;138-13962-68
Preliminary Comparison of Non-Fourier Methods for Spectrum Analysis of Nonuniformly Sampled NMR Data.
Maciejewski, M.W., Dashti, H., Zambrello, M.A., Schuyler, A.D., Mobli, M., Eghbalnia, H.R., Delaglio, F., Stern, A.S., & Hoch, J.C. Nist.gov Publications 2018 Jan;
Practical Nonuniform Sampling and Non-Fourier Spectral Reconstruction for Multidimensional NMR.
Maciejewski, Mark W; Schuyler, Adam D; Hoch, Jeffrey C Methods in molecular biology (Clifton, N.J.) 2018 Jan;1688341-352
Robust and transferable quantification of NMR spectral quality using IROC analysis.
Zambrello, Matthew A; Maciejewski, Mark W; Schuyler, Adam D; Weatherby, Gerard; Hoch, Jeffrey C Journal of magnetic resonance (San Diego, Calif. : 1997) 2017 Oct;28537-46
NMRbox: A Resource for Biomolecular NMR Computation.
Maciejewski, Mark W; Schuyler, Adam D; Gryk, Michael R; Moraru, Ion I; Romero, Pedro R; Ulrich, Eldon L; Eghbalnia, Hamid R; Livny, Miron; Delaglio, Frank; Hoch, Jeffrey C Biophysical journal 2017 Apr;112(8):1529-1534
Nuclear Magnetic Resonance Structures of GCN4p Are Largely Conserved When Ion Pairs Are Disrupted at Acidic pH but Show a Relaxation of the Coiled Coil Superhelix.
Kaplan, Anne R; Brady, Megan R; Maciejewski, Mark W; Kammerer, Richard A; Alexandrescu, Andrei T Biochemistry 2017 Mar;56(11):1604-1619
NMR assignments for the insertion domain of bacteriophage CUS-3 coat protein.
Tripler, Therese N; Maciejewski, Mark W; Teschke, Carolyn M; Alexandrescu, Andrei T Biomolecular NMR assignments 2015 Oct;9(2):333-6
Nonuniform sampling of hypercomplex multidimensional NMR experiments: Dimensionality, quadrature phase and randomization.
Schuyler, Adam D; Maciejewski, Mark W; Stern, Alan S; Hoch, Jeffrey C Journal of magnetic resonance (San Diego, Calif. : 1997) 2015 May;254121-30
Analysis of metabolism in dormant spores of Bacillus species by 31P nuclear magnetic resonance analysis of low-molecular-weight compounds.
Ghosh, Sonali; Korza, George; Maciejewski, Mark; Setlow, Peter Journal of bacteriology 2014 Dec;197(5):992-1001
Nonuniform sampling and maximum entropy reconstruction in multidimensional NMR.
Hoch, Jeffrey C; Maciejewski, Mark W; Mobli, Mehdi; Schuyler, Adam D; Stern, Alan S Accounts of chemical research 2014 Jan;47(2):708-17
NMR mapping of PCNA interaction with translesion synthesis DNA polymerase Rev1 mediated by Rev1-BRCT domain.
Pustovalova, Yulia; Maciejewski, Mark W; Korzhnev, Dmitry M Journal of molecular biology 2013 Sep;425(17):3091-105
Crystal structure of the ubiquitin-like small archaeal modifier protein 2 from Haloferax volcanii.
Li, Yunfeng; Maciejewski, Mark W; Martin, Jonathan; Jin, Kai; Zhang, Yuhang; Maupin-Furlow, Julie A; Hao, Bing Protein science : a publication of the Protein Society 2013 Sep;22(9):1206-17
Serine lipids of Porphyromonas gingivalis are human and mouse Toll-like receptor 2 ligands.
Clark, Robert B; Cervantes, Jorge L; Maciejewski, Mark W; Farrokhi, Vahid; Nemati, Reza; Yao, Xudong; Anstadt, Emily; Fujiwara, Mai; Wright, Kyle T; Riddle, Caroline; La Vake, Carson J; Salazar, Juan C; Finegold, Sydney; Nichols, Frank C Infection and immunity 2013 Sep;81(9):3479-89
(1)H, (13)C, and (15)N chemical shift assignments for PfPMT, a phosphoethanolamine methyltransferase from Plasmodium falciparum.
Bezsonova, Irina; Rujan, Iulian; Bobenchik, April M; Gorbatyuk, Vitaliy; Maciejewski, Mark W; Gorbatyuk, Oksana; Hao, Bing; Arthanari, Haribabu; Mamoun, Choukri Ben; Hoch, Jeffrey C Biomolecular NMR assignments 2013 Apr;7(1):17-20
Formalism for hypercomplex multidimensional NMR employing partial-component subsampling.
Schuyler, Adam D; Maciejewski, Mark W; Stern, Alan S; Hoch, Jeffrey C Journal of magnetic resonance (San Diego, Calif. : 1997) 2013 Feb;22720-4
Sparse sampling methods in multidimensional NMR.
Mobli, Mehdi; Maciejewski, Mark W; Schuyler, Adam D; Stern, Alan S; Hoch, Jeffrey C Physical chemistry chemical physics : PCCP 2012 Aug;14(31):10835-43
Data sampling in multidimensional NMR: fundamentals and strategies.
Maciejewski, Mark W; Mobli, Mehdi; Schuyler, Adam D; Stern, Alan S; Hoch, Jeffrey C Topics in current chemistry 2012 Jan;31649-77
Random phase detection in multidimensional NMR.
Maciejewski, Mark W; Fenwick, Matthew; Schuyler, Adam D; Stern, Alan S; Gorbatyuk, Vitaliy; Hoch, Jeffrey C Proceedings of the National Academy of Sciences of the United States of America 2011 Oct;108(40):16640-4
Knowledge-based nonuniform sampling in multidimensional NMR.
Schuyler, Adam D; Maciejewski, Mark W; Arthanari, Haribabu; Hoch, Jeffrey C Journal of biomolecular NMR 2011 Jul;50(3):247-62
Nonuniform sampling and spectral aliasing.
Maciejewski, Mark W; Qui, Harry Z; Rujan, Iulian; Mobli, Mehdi; Hoch, Jeffrey C Journal of magnetic resonance (San Diego, Calif. : 1997) 2009 Jul;199(1):88-93
A proposed syntax for Minimotif Semantics, version 1.
Vyas, Jay; Nowling, Ronald J; Maciejewski, Mark W; Rajasekaran, Sanguthevar; Gryk, Michael R; Schiller, Martin R BMC genomics 2009 Jan;10360
Minimotif miner 2nd release: a database and web system for motif search.
Rajasekaran, Sanguthevar; Balla, Sudha; Gradie, Patrick; Gryk, Michael R; Kadaveru, Krishna; Kundeti, Vamsi; Maciejewski, Mark W; Mi, Tian; Rubino, Nicholas; Vyas, Jay; Schiller, Martin R Nucleic acids research 2009 Jan;37(Database issue):D185-90
Randomization improves sparse sampling in multidimensional NMR.
Hoch, Jeffrey C; Maciejewski, Mark W; Filipovic, Blagoje Journal of magnetic resonance (San Diego, Calif. : 1997) 2008 Aug;193(2):317-20
Automatic maximum entropy spectral reconstruction in NMR.
Mobli, Mehdi; Maciejewski, Mark W; Gryk, Michael R; Hoch, Jeffrey C Journal of biomolecular NMR 2007 Oct;39(2):133-9
Mapping the phosphoinositide-binding site on chick cofilin explains how PIP2 regulates the cofilin-actin interaction.
Gorbatyuk, Vitaliy Y; Nosworthy, Neil J; Robson, Scott A; Bains, Naresh P S; Maciejewski, Mark W; Dos Remedios, Cris G; King, Glenn F Molecular cell 2006 Nov;24(4):511-22
Regulation of RhoGEF activity by intramolecular and intermolecular SH3 domain interactions.
Schiller, Martin R; Chakrabarti, Kausik; King, Glenn F; Schiller, Noraisha I; Eipper, Betty A; Maciejewski, Mark W The Journal of biological chemistry 2006 Jul;281(27):18774-86
Levels of glycine betaine in growing cells and spores of Bacillus species and lack of effect of glycine betaine on dormant spore resistance.
Loshon, Charles A; Wahome, Paul G; Maciejewski, Mark W; Setlow, Peter Journal of bacteriology 2006 Apr;188(8):3153-8
Minimotif Miner: a tool for investigating protein function.
Balla, Sudha; Thapar, Vishal; Verma, Snigdha; Luong, Thaibinh; Faghri, Tanaz; Huang, Chun-Hsi; Rajasekaran, Sanguthevar; del Campo, Jacob J; Shinn, Jessica H; Mohler, William A; Maciejewski, Mark W; Gryk, Michael R; Piccirillo, Bryan; Schiller, Stanley R; Schiller, Martin R Nature methods 2006 Mar;3(3):175-7
High-performance exact algorithms for motif search.
Rajasekaran, Sanguthevar; Balla, Sudha; Huang, Chun-Hsi; Thapar, Vishal; Gryk, Michael; Maciejewski, Mark; Schiller, Martin Journal of clinical monitoring and computing 2005 Oct;19(4-5):319-28
Backbone and side-chain 1H, 15N, and 13C assignments for the beta domain of the bacterial cell division protein DivIB.
Robson, Scott A; Gorbatyuk, Vitaliy Y; Maciejewski, Mark W; King, Glenn F Journal of biomolecular NMR 2005 Mar;31(3):261-2
Solution structure of the Tctex1 dimer reveals a mechanism for dynein-cargo interactions.
Wu, Hongwei; Maciejewski, Mark W; Takebe, Sachiko; King, Stephen M Structure (London, England : 1993) 2005 Feb;13(2):213-23
Stability of loops in the structure of lactose permease.
Bennett, Michael; Yeagle, James A; Maciejewski, Mark; Ocampo, James; Yeagle, Philip L Biochemistry 2004 Oct;43(40):12829-37
Structural studies of the putative helix 8 in the human beta(2) adrenergic receptor: an NMR study.
Katragadda, M; Maciejewski, M W; Yeagle, P L Biochimica et biophysica acta 2004 May;1663(1-2):74-81
Structure and mechanism of action of Sda, an inhibitor of the histidine kinases that regulate initiation of sporulation in Bacillus subtilis.
Rowland, Susan L; Burkholder, William F; Cunningham, Katherine A; Maciejewski, Mark W; Grossman, Alan D; King, Glenn F Molecular cell 2004 Mar;13(5):689-701
Modulation of agrin function by alternative splicing and Ca2+ binding.
Stetefeld, Jörg; Alexandrescu, Andrei T; Maciejewski, Mark W; Jenny, Margrit; Rathgeb-Szabo, Klara; Schulthess, Therese; Landwehr, Ruth; Frank, Sabine; Ruegg, Markus A; Kammerer, Richard A Structure (London, England : 1993) 2004 Mar;12(3):503-15
Relaxation-based structure refinement and backbone molecular dynamics of the dynein motor domain-associated light chain.
Wu, Hongwei; Blackledge, Martin; Maciejewski, Mark W; Mullen, Gregory P; King, Stephen M Biochemistry 2003 Jan;42(1):57-71
Mapping of the interaction interface of DNA polymerase beta with XRCC1.
Gryk, Michael R; Marintchev, Assen; Maciejewski, Mark W; Robertson, Anthony; Wilson, Samuel H; Mullen, Gregory P Structure (London, England : 1993) 2002 Dec;10(12):1709-20
Backbone and side-chain 1H, 15N, and 13C assignments for chick cofilin.
Bains, Naresh P S; Gorbatyuk, Vitaliy Y; Nosworthy, Neil J; Robson, Scott A; Maciejewski, Mark W; dos Remedios, Cristobal G; King, Glenn F Journal of biomolecular NMR 2002 Feb;22(2):193-4
Solution structure of a viral DNA repair polymerase.
Maciejewski, M W; Shin, R; Pan, B; Marintchev, A; Denninger, A; Mullen, M A; Chen, K; Gryk, M R; Mullen, G P Nature structural biology 2001 Nov;8(11):936-41
Solution structure of the catalytic domain of gammadelta resolvase. Implications for the mechanism of catalysis.
Pan, B; Maciejewski, M W; Marintchev, A; Mullen, G P Journal of molecular biology 2001 Jul;310(5):1089-107
Structural basis for the topological specificity function of MinE.
King, G F; Shih, Y L; Maciejewski, M W; Bains, N P; Pan, B; Rowland, S L; Mullen, G P; Rothfield, L I Nature structural biology 2000 Nov;7(11):1013-7
Solution structure of a dynein motor domain associated light chain.
Wu, H; Maciejewski, M W; Marintchev, A; Benashski, S E; Mullen, G P; King, S M Nature structural biology 2000 Jul;7(7):575-9
Discovery and characterization of a family of insecticidal neurotoxins with a rare vicinal disulfide bridge.
Wang, X; Connor, M; Smith, R; Maciejewski, M W; Howden, M E; Nicholson, G M; Christie, M J; King, G F Nature structural biology 2000 Jun;7(6):505-13
Backbone dynamics and refined solution structure of the N-terminal domain of DNA polymerase beta. Correlation with DNA binding and dRP lyase activity.
Maciejewski, M W; Liu, D; Prasad, R; Wilson, S H; Mullen, G P Journal of molecular biology 2000 Feb;296(1):229-53
Solution structure of the single-strand break repair protein XRCC1 N-terminal domain.
Marintchev, A; Mullen, M A; Maciejewski, M W; Pan, B; Gryk, M R; Mullen, G P Nature structural biology 1999 Sep;6(9):884-93
Functional analysis of the amino-terminal 8-kDa domain of DNA polymerase beta as revealed by site-directed mutagenesis. DNA binding and 5'-deoxyribose phosphate lyase activities.
Prasad, R; Beard, W A; Chyan, J Y; Maciejewski, M W; Mullen, G P; Wilson, S H The Journal of biological chemistry 1998 May;273(18):11121-6
Structure of a compact peptide from staphylococcal nuclease determined by circular dichroism and NMR spectroscopy.
Maciejewski, M W; Zehfus, M H Biochemistry 1995 May;34(17):5795-800

Book Sections

Nonuniform sampling in biomolecular NMR
Maciejewski MW, Stern AS, King GF, Hoch JC Modern Magnetic Resonance 2006 Jan;1287-1293

Conference Papers

When Should FAIR Begin? Applying FAIR During Data Creation
Michael R Gryk ; Adam Schuyler ; Jon Wedell ; Mark Maciejewski ; Gerard Weatherby ; Pedro Romero 2019 Jun;426-427
Insights into the mechanism of the β-elimination catalyzed by the N-terminal domain of DNA polymerase β
Mullen G.P., Antuch W., Maciejewski M.W., Prasad R., Wilson S.H. Tetrahedron 1997 Jan;53(35):12057-12066

Letters

Another barrier to reproducibility
MW Maciejewski, AD Schuyler, MR Gryk, II Moraru, PR Romero, EL Ulrich, HR Eghbalnia, M Livny, F Delaglio and JC Hoch Science 2017 Jan;354(6317):1240-1241
An automated tool for maximum entropy reconstruction of biomolecular NMR spectra.
Mobli, Mehdi; Maciejewski, Mark W; Gryk, Michael R; Hoch, Jeffrey C Nature methods 2007 Jun;4(6):467-8
1H, 13C and 15N resonance assignments for the perdeuterated 22 kD palm-thumb domain of DNA polymerase beta.
Gryk, Michael R; Maciejewski, Mark W; Robertson, Anthony; Mullen, Mary A; Wilson, Samuel H; Mullen, Gregory P Journal of biomolecular NMR 2002 Feb;22(2):197-8
1H, 15N, and 13C resonance assignments for a 20 kDa DNA polymerase from African swine fever virus.
Maciejewski, M W; Pan, B; Shin, R; Denninger, A; Mullen, G P Journal of biomolecular NMR 2001 Oct;21(2):177-8
1H, 13C and 15N backbone assignments for the C-terminal globular domain of agrin.
Alexandrescu, A T; Maciejewski, M W; Rüegg, M A; Engel, J; Kammerer, R A Journal of biomolecular NMR 2001 Jul;20(3):295-6
1H, 15N and 13C resonance assignments for the Tctex1 dynein light chain from Chlamydomonas flagella.
Wu, H; Maciejewski, M W; Benashski, S E; Mullen, G P; King, S M Journal of biomolecular NMR 2001 May;20(1):89-90
1H, 15N, and 13C resonance assignments for the N-terminal 20 kDa domain of the DNA single-strand break repair protein XRCC1.
Marintchev, A; Maciejewski, M W; Mullen, G P Journal of biomolecular NMR 1999 Apr;13(4):393-4
Backbone and side-chain 1H, 15N, and 13C assignments for the topological specificity domain of the MinE cell division protein.
King, G F; Pan, B; Maciejewski, M W; Rowland, S L; Rothfield, L I; Mullen, G P Journal of biomolecular NMR 1999 Apr;13(4):395-6
1H, 15N and 13C resonance assignments for the 22 kDa LC1 light chain from Chlamydomonas outer arm dynein.
Wu, H; Maciejewski, M W; Benashski, S E; Mullen, G P; King, S M Journal of biomolecular NMR 1999 Mar;13(3):309-10

Notes

Comment on "magnetic resonance spectroscopy identifies neural progenitor cells in the live human brain".
Hoch, Jeffrey C; Maciejewski, Mark W; Gryk, Michael R Science (New York, N.Y.) 2008 Aug;321(5889):640

Reviews

Biomolecular NMR data analysis
Gryk M.R., Vyas J., Maciejewski M.W. Progress in Nuclear Magnetic Resonance Spectroscopy 2010 Jan;56(4):329-345