Mark R. Terasaki, PhDAssociate Professor, Department of Cell Biology
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I have been using advanced light microscopy techniques to investigate intracellular organization and function. Most of my work has involved imaging of living cells using confocal microscopy. I have recently completed work using green fluorescent protein chimeras to label the ER and Golgi apparatus during the cell cycle in sea urchin embryos. Three current projects are: how cells heal wounds in the plasma membrane; changes in ER structure during meiotic maturation; and nuclear envelope breakdown, where we are planning to use some computer modeling and two photon microscopy to understand steps involved in this process.
Accepting Lab Rotation Students: Fall 2022 and Spring 2023
Lab Rotation Projects Terasaki/Fein Lab Cell Wounding Project We have developed an electrophysiological method for studying plasma membrane wound repair in single cells. Large plasma membrane disruptions in starfish oocytes, made by femtosecond pulses from a Ti-Sapphire laser, are repaired within a few seconds as shown by stabilization of membrane electrical properties and restoration of dye exclusion. The membrane potential after wounding is sensitive to the extracellular Cl- concentration but not to that of Na+, K+ or H+ indicating that Cl- permeable intracellular membranes have fused with the plasma membrane. We believe that cell wounding provides a novel means for the electrophysiological analysis of chloride permeable intracellular membranes that have been translocated to the plasma membrane.
Journal Articles
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Actin cytoskeleton and associated myosin motors within the renal epithelium.
American journal of physiology. Renal physiology 2024 Jul;
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Mitochondria-ER contact sites expand during mitosis.
iScience 2024 Apr;27(4):109379
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Evidence for glia-mediated, age-dependent remodeling of myelin at the axon initial segment.
The Journal of comparative neurology 2024 Feb;532(2):e25574
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Cytonemes with complex geometries and composition extend into invaginations of target cells.
The Journal of cell biology 2021 May;220(5):
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Gap junction internalization and processing in vivo: a 3D immune-electron microscopy study
Journal of Cell Science 2021 Jan;
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Gap junction internalization and processing in vivo: a 3D immuno-electron microscopy study.
Journal of cell science 2021 Jan;134(1):jcs252726
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Analysis of the three dimensional structure of the kidney glomerulus capillary network.
Scientific reports 2020 Nov;10(1):20334
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Odontoblast processes of the mouse incisor are plates oriented in the direction of growth.
Anatomical record (Hoboken, N.J. : 2007) 2020 Nov;
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Cellular heterogeneity of the LH receptor and its significance for cyclic GMP signaling in mouse preovulatory follicles.
Endocrinology 2020 May;161(7)bgaa074
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Ultrastructure of primary pacemaking cells in rabbit sino-atrial node cells indicates limited sarcoplasmic reticulum content.
FASEB bioAdvances 2020 Feb;2(2):106-115
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Extraocular, rod-like photoreceptors in a flatworm express xenopsin photopigment.
eLife 2019 Oct;8
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Three-dimensional organization of transzonal projections and other cytoplasmic extensions in the mouse ovarian follicle.
Scientific reports 2019 Feb;9(1):1262
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Serial-section electron microscopy using automated tape-collecting ultramicrotome (ATUM).
Methods in cell biology 2019 Jan;15241-67
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Dynamic cytoplasmic projections connect mammalian spermatogonia in vivo.
Development (Cambridge, England) 2018 Jul;145
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Axonal endoplasmic reticulum is very narrow.
Journal of cell science 2018 Feb;131(4):
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Anteroposterior axis patterning by early canonical Wnt signaling during hemichordate development.
PLoS biology 2018 Jan;16(1):e2003698
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Modeling of axonal endoplasmic reticulum network by spastic paraplegia proteins.
eLife 2017 Jul;6
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Localization of phosphorylated connexin 43 by serial section immunogold electron microscopy.
Journal of cell science 2017 Feb;1301333-1340
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A finer look at a fine cellular meshwork.
Science (New York, N.Y.) 2016 Oct;354(6311):415-416
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Stacked endoplasmic reticulum sheets are connected by helicoidal membrane motifs.
Cell 2013 Jul;154(2):285-96
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Two-stage dependence for 1-methyladenine induced reinitiation of meiotic maturation in starfish oocytes.
Experimental cell research 2010 Oct;316(16):2654-63
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Dorsoventral patterning in hemichordates: insights into early chordate evolution.
PLoS biology 2006 Sep;4(9):e291
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Quantification of fluorescence in thick specimens, with an application to cyclin B-GFP expression in starfish oocytes.
Biology of the cell / under the auspices of the European Cell Biology Organization 2006 Apr;98(4):245-52
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A contractile nuclear actin network drives chromosome congression in oocytes.
Nature 2005 Aug;436(7052):812-8
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Rapid increase in plasma membrane chloride permeability during wound resealing in starfish oocytes.
The Journal of general physiology 2005 Aug;126(2):151-9
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Second harmonic imaging of exocytosis at fertilization.
Biophysical journal 2005 Jun;88(6):L46-8
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Multiphoton-excited microfabrication in live cells via Rose Bengal cross-linking of cytoplasmic proteins.
Optics letters 2005 Jan;30(2):159-61
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Redistribution of the kinesin-II subunit KAP from cilia to nuclei during the mitotic and ciliogenic cycles in sea urchin embryos.
Developmental biology 2004 Oct;274(1):56-69
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Multiphoton excited microfabrication in live cells via rose bengal crosslinking of cytoplasmic proteins
Optics Lett. 2004 Jan;30159-161
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Cyclin aggregation and robustness of bio-switching.
Molecular biology of the cell 2003 Nov;14(11):4695-706
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Localization and dynamics of Cdc2-cyclin B during meiotic reinitiation in starfish oocytes.
Molecular biology of the cell 2003 Nov;14(11):4685-94
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Controlled damage in thick specimens by multiphoton excitation.
Molecular biology of the cell 2003 May;14(5):1808-17
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Nuclear envelope breakdown in starfish oocytes proceeds by partial NPC disassembly followed by a rapidly spreading fenestration of nuclear membranes.
The Journal of cell biology 2003 Mar;160(7):1055-68
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Three-dimensional high-resolution second-harmonic generation imaging of endogenous structural proteins in biological tissues.
Biophysical journal 2002 Jan;82(1 Pt 1):493-508
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Fluorescent staining of subcellular organelles: ER, Golgi complex, and mitochondria.
Current protocols in cell biology / editorial board, Juan S. Bonifacino ... [et al.] 2001 May;Chapter 4Unit 4.4
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Changes in organization of the endoplasmic reticulum during Xenopus oocyte maturation and activation.
Molecular biology of the cell 2001 Apr;12(4):1103-16
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A new model for nuclear envelope breakdown.
Molecular biology of the cell 2001 Feb;12(2):503-10
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Local inhibition of cortical rotation in Xenopus eggs by an anti-KRP antibody.
Developmental biology 2000 Aug;224(2):250-62
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Evidence that fertilization activates starfish eggs by sequential activation of a Src-like kinase and phospholipase cgamma.
The Journal of biological chemistry 2000 Jun;275(22):16788-94
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Patching plasma membrane disruptions with cytoplasmic membrane.
Journal of cell science 2000 Jun;113 ( Pt 11)(11):1891-902
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Dynamics of the endoplasmic reticulum and golgi apparatus during early sea urchin development.
Molecular biology of the cell 2000 Mar;11(3):897-914
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Ran is associated with chromosomes during starfish oocyte meiosis and embryonic mitoses.
Zygote (Cambridge, England) 2000 Jan;8 Suppl 1(SUPPL.):S91
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The cortical endoplasmic reticulum (ER) of the mouse egg: localization of ER clusters in relation to the generation of repetitive calcium waves.
Developmental biology 1999 Nov;215(2):431-42
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Requirement of a Src family kinase for initiating calcium release at fertilization in starfish eggs.
The Journal of biological chemistry 1999 Oct;274(41):29318-22
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Identification of PLCgamma-dependent and -independent events during fertilization of sea urchin eggs.
Developmental biology 1999 Feb;206(2):232-47
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Calcium release at fertilisation in starfish eggs is mediated by phospholipase Cγ
Zygote 1998 Jan;6(1 SUPPL.):
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Golgi tubule traffic and the effects of brefeldin A visualized in living cells.
The Journal of cell biology 1997 Dec;139(5):1137-55
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Large plasma membrane disruptions are rapidly resealed by Ca2+-dependent vesicle-vesicle fusion events.
The Journal of cell biology 1997 Oct;139(1):63-74
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Calcium release at fertilization in starfish eggs is mediated by phospholipase Cgamma.
The Journal of cell biology 1997 Sep;138(6):1303-11
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Structural change of the endoplasmic reticulum during fertilization: evidence for loss of membrane continuity using the green fluorescent protein.
Developmental biology 1996 Nov;179(2):320-8
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Diffusional mobility of Golgi proteins in membranes of living cells.
Science (New York, N.Y.) 1996 Aug;273(5276):797-801
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Actin filament translocations in sea urchin eggs.
Cell motility and the cytoskeleton 1996 Jan;34(1):48-56
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Transport of cytoskeletal elements in the squid giant axon.
Proceedings of the National Academy of Sciences of the United States of America 1995 Dec;92(25):11500-3
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Direct membrane retrieval into large vesicles after exocytosis in sea urchin eggs.
The Journal of cell biology 1995 Dec;131(5):1183-92
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Continuous network of endoplasmic reticulum in cerebellar Purkinje neurons.
Proceedings of the National Academy of Sciences of the United States of America 1994 Aug;91(16):7510-4
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Structural changes in the endoplasmic reticulum of starfish oocytes during meiotic maturation and fertilization.
Developmental biology 1994 Aug;164(2):579-87
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Structural changes of the endoplasmic reticulum of sea urchin eggs during fertilization.
Developmental biology 1993 Apr;156(2):566-73
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Evidence for glia-mediated, age-dependent remodeling of myelin at the axon initial segment. (in press)
Journal of Comparative Neurology
Book Chapters
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Ultrastructural Analysis of Cell-Cell Interactions in Drosophila Ovary.
Methods in molecular biology (Clifton, N.J.) 2021 Jan;234679-90
Editorials
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Editorial.
Seminars in cell & developmental biology 2022 Sep;12961-62
Reviews
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Bio-switches: what makes them robust?
Current opinion in genetics & development 2004 Aug;14(4):428-34
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Labeling of cell membranes and compartments for live cell fluorescence microscopy.
Methods in cell biology 2004 Jan;74(74):469-89
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Quantitative microinjection of oocytes, eggs, and embryos.
Methods in cell biology 2004 Jan;74(74):219-42
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Chromosomal association of Ran during meiotic and mitotic divisions.
Journal of cell science 2002 Dec;115(Pt 23):4685-93
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Coping with the inevitable: how cells repair a torn surface membrane.
Nature cell biology 2001 May;3(5):E124-9
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Imaging of echinoderm fertilization.
Molecular biology of the cell 1998 Jul;9(7):1609-12