Peter F. Maye, Ph.D.Associate Professor, Reconstructive Sciences
Skeletal Development & Regeneration
Research in my lab has a great interest in understanding signaling mechanisms that influence the fate of skeletal progenitors during the development, maintenance, and regeneration of bone tissues. Ongoing work includes the use of mouse and human embryonic stem cells and induced pluripotent stem (iPS) cells and learning how to direct their differentiation into cells of the axial skeleton. We also investigate adult bone marrow mesenchymal stem cells (MSCs) and the signaling pathways that influence their fate and function.
Rare Skeletal Diseases
Research in my lab is also interested in different skeletal dysplasia that impact bone tissues. We have become very interested in a rare craniofacial disorder known as cherubism. Currently, this work is focused on understanding how the Transforming Growth Factor Beta signaling pathway contributes to the pathology of this disease.
Differentiation of embyronic stem cells and iPS cells into mature skeletal cell types
Understanding how to generate skeletal progenitors from pluripotent stem cells has potentially great value for understanding the molecular basis of skeletal diseases and for the field of regenerative medicine. Currently, the field is beginning to make real progress in understanding how to differentiate pluripotent stem cells in a very defined way into skeletal progenitors. However, the cell types generated in vitro are very heterogeneous and their functional validation remains poorly tested. With this in mind, part of our strategy involves genetically engineering mouse and human ES and iPS cells with fluorescent reporters that enable us to visualize and study the biology of distinct skeletal progenitors generated in vitro. In conjunction with different reporter stem cell lines, we are investigating how different signaling pathways influence the differentiation of pluripotent stem cells into paraxial mesoderm. We are very interested in how to make paraxial mesoderm cells because they have the potential to give rise to wide breadth of mature skeletal cell types including, osteoblast, chondrocyte, tenocyte, myoblast, and dermal fibroblast.
Cherubism and Transforming Growth Factor (TGF) Beta Signaling
Cherubism is a rare autosomal dominant craniofacial disorder affecting pre-pubertal children, which is characterized by multilocular lesions in the mandible and/or maxilla consisting of numerous giant osteoclasts and extensive fibrous-osseous tissue hyperplasia. Currently, there is no accepted treatment for this disease. Based on certain characteristics of the cherubism phenotype, we suspected that increased TGFβ signaling may have a key role in the presentation of this disease and have used a mouse model of Cherubism to investigate this possibility. Our ongoing studies are very encouraging and provide evidence that reducing TGFbeta signaling may be an effective therapuetic approach to treat patients.
Accepting Lab Rotation Students: Fall '20, Spring '21
|Title or Abstract||Type||Sponsor/Event||Date/Year||Location|
|Murine Animal Models to Interrogate the Function and Commitment of Skeletal Progenitors||Talk||New England Musculoskeletal Institute Research Day||2016||Farmington, CT|
|Understanding the Coordinated Development of Bone Tissue and the Bone Marrow||Lecture||University of Hartford||2016||West Hartford, CT|
|Cherubism and Transforming Growth Factor Beta Signaling||Talk||Dental Dean's Lecture Series||2016||Farmington, CT|
|Development of Genetic Models to Study Cartilage Biology||Talk||New England Musculoskeletal Institute Research Day||2014||Farmington, CT|
|Genetic Approaches to Study Adult Bone Marrow Skeletal Progenitors||Talk||Connecticut Science Festival Stem Cell Panelist||2014||Farmington, CT|
|Mouse Transgenesis: Approaches to Genetically Engineer Mice||Lecture||University of Hartford||2014||West Hartford, CT|
|Fate Mapping with Osterix Cre Mice Reveals the Origin and Contribution of Bone Marrow Mesenchymal Stem Cells||Talk||American Society for Bone and Mineral Research Meeting||2012||Minneapolis, MN|
|Differentiating Embryonic Stem Cells Down the Axial Skeletal Lineage||Lecture||Central Connecticut State University||2012||New Britain, CT|
|Differentiating Embryonic Stem Cells Down the Axial Skeletal Lineage||Talk||Stem Cells and Regenerative Biology Symposium, UCONN Health||2011||Farmington, CT|
|New Fluorescent Protein Reporter Animal Models to Study Skeletal Biology||Poster||ASBMR||2010||Toronto, Canada|
|The Application of Transgenic Reporter Mice to Study Skeletal Cell Types||Talk||Dental Dean's Seminar Series||2010||Farmington, CT|
|Characterizing the Osteogenic Potential of Mesenchymal Stem Cells and Their Immediate Cellular Derivatives||Poster||American Society for Bone and Mineral Research||2009||Denver, CO|
|The Next Generation of Fluorescent Protein Reporter Animal Models to Mark Skeletal Cell Types||Talk||Regenerative Engineering Workshop||2009||Farmington, CT|
|Dermo1 Lineage Tracing Identifies Early Osteoprogenitor Cells in Adult Murine Bone Marrow Mesenchymal Stem Cell Cultures||Poster||ASBMR||2008||Montreal, Canada|
|Engineering Mice with Multiple BAC Fluorescent Protein Reporter Gene Elements||Poster||American Society for Bone and Mineral Research||2008||Montreal, Canada|
|Engineering Mice with Multiple Reporter Gene Elements||Talk||New England Musculoskeletal Institute Research Day||2008||Farmington, CT|
|Engineering Mice with Multiple BAC Fluorescent Protein Reporter Gene Elements||Poster||Endocrine Society /Endocrine Fellows Forum||2007||Honolulu, Hawaii|
|A Bacterial Recombination Strategy to Generate Linked Multiple Gene Reporter Constructs||Poster||IADR||2007||New Orleans, LA|
|A Bacterial Recombination Strategy to Link Multiple Gene Reporter Constructs||Poster||StemConn||2007||Hartford, CT|
|Characterization of LRP5 Mutant Osteoblast Cultures using Collagen 1a1 GFP Reporter Mice||Poster||ASBMR||2006||Philadelphia, PA|
|Haploinsufficiency of Beta-Catenin Results in Reduced Bone Mass||Poster||ASBMR||2005||Nashville, Tennessee|