Zhichao Fan, Ph.D.Assistant Professor of Immunology
- Education & Training
- Committees & Organizations
- Research Opportunities
- Lab Rotations
Dr. Zhichao Fan is an Assistant Professor of Immunology whose research lies in the field of innate immunology and inflammation. Specifically, his research focuses on the molecular mechanism of integrin activation on leukocyte adhesion during inflammatory diseases, including myocardial ischemia-reperfusion injury and cystic fibrosis, which he examines using advanced microscopy, including super-resolution single molecular microscopy, intravital microscopy, qDF microscopy, and IVFC.
|Ph.D.||Fudan University||Chemical Biology|
|Postdoctoral||La Jolla Institute for Immunology||Immunology|
|Name of Award/Honor||Awarding Organization|
|2021 AAI Early Career Faculty Grant||American Association of Immunologists|
|2020 AAI Early Career Faculty Travel Grant||American Association of Immunologists|
|2020 Cell & Molecular Physiology Section New Investigator Award||American Physiological Society|
|2019 SLB Meeting Travel Award||Society for Leukocyte Biology|
|R01 Research Project Grant||National Institutes of Health|
|2019 AAI Early Career Faculty Travel Grant||American Association of Immunologists|
|Career Development Award||American Heart Association|
|2017 Pappenheimer Postdoctoral Travel Award||The Microcirculatory Society|
|WSA Postdoctoral Fellowship||American Heart Association|
|2016 Pappenheimer Postdoctoral Travel Award||The Microcirculatory Society|
|2016 AAI Trainee Abstract Award||American Association of Immunologists|
|2015 AAI Young Investigator Award at the La Jolla Immunology Conference||American Association of Immunologists|
|Name & Description||Category||Role||Type||Scope||Start Year||End Year|
|Frontiers in Cell and Developmental Biology||Editorial Board||Associate Editor||External||International||2021|
|Biomolecules||Editorial Board||Guest Editor||External||International||2021|
|Frontiers in Pharmacology||Editorial Board||Guest Associate Editor||External||International||2021|
|Frontiers in Immunology||Editorial Board||Guest Associate Editor||External||International||2021|
|Frontiers in Cellular and Infection Microbiology||Editorial Board||Review Editor||External||International||2021|
|American Physiological Society, the Cell and Molecular Physiology Section||Professional/Scientific Organization||Chair of Nominating Committee||External||International||2021|
|Virtual Immunology 2021||Workshop/Conference||Block Symposium Chair/Moderator||External||International||2021||2021|
|Medical Research Council||Professional/Scientific Organization||Grant Reviewer||External||International||2021|
|the Journal of Immunology||Editorial Board||Associate Editor||External||International||2020|
|Frontiers in Cell and Developmental Biology||Editorial Board||Guest Associate Editor||External||International||2020||2021|
|Microcirculation||Editorial Board||Editorial Board||External||International||2020|
|Society of Leukocyte Biology||Professional/Scientific Organization||Member||External||International||2019|
|Biomedical Engineering Society||Professional/Scientific Organization||Member||External||International||2018|
|American Society for Cell Biology||Professional/Scientific Organization||Member||External||International||2018|
|American Physiological Society||Professional/Scientific Organization||Member||External||International||2018|
|American Association of Immunologists||Professional/Scientific Organization||Member||External||International||2015|
|American Heart Association||Professional/Scientific Organization||Member||External||International||2015|
|Microcirculatory Society||Professional/Scientific Organization||Member||External||International||2015|
Neutrophils are the most abundant population of human leukocytes (~60%) and play critical roles in infections and inflammation. Circulating neutrophils are recruited to inflamed or infected tissue. The recruitment cascade is initiated by selectin-mediated rolling and chemokine-triggered integrin-dependent arrest (firm adhesion) of neutrophils on the vascular endothelium under flow. Thus, rolling and arrest are two major processes to target for fighting against inflammatory diseases (decrease rolling and arrest) and infections (increase rolling and arrest).
My long-term research interests involve the development of a comprehensive understanding of the mechanism of integrin activation and leukocyte recruitment and how they contribute to human disease, especially inflammation in cardiovascular diseases, which I examine using advanced microscopy, including super-resolution single molecular microscopy, intravital microscopy, and quantitative dynamic footprinting microscopy. My current ongoing projects include:
1. Molecular mechanism and clinical relevance of the auto-inhibitory in-cis interaction of integrin and their ligands;
2. Identifying new molecules involved in the pathway of integrin activation and clustering;
3. Pharmacology test of drugs targeting integrins in vitro and in disease models (myocardial ischemia-reperfusion injury)
4. High-throughput screening of drugs inhibiting integrin activation and inflammation.
5. Monitoring of leukocyte dynamics in diseases using intravital microscopy and in vivo flow cytometry (IVFC).
NIH-supported Postdoctoral Fellow Positions in Leukocyte Biology and Immunology at the University of Connecticut School of Medicine (UConn Health)
Our laboratory is looking for motivated postdoctoral fellows to study integrin activation and leukocyte trafficking in inflammatory diseases using advanced microscopy, such as super-resolution microscopy, high-resolution live-cell imaging, and intravital microscopy.
We work on primary human leukocytes, leukocyte-like cell lines, and gene-edited mice to answer questions regarding integrin activation and leukocyte trafficking. Multiple advanced microscopy, from single-molecular level super-resolution microscopy to physiological-relevant intravital microscopy, will be used.
The PI is a faculty member of the Department of Immunology at UConn Health (https://health.uconn.edu/immunology/). This is an exciting research environment to cultivate new research ideas and opportunities. The PI has received an R01 award from NIH and a startup fund to support our research. The candidate will receive excellent guidance on scientific research as well as career development (grants, awards, conferences, etc.).
Qualified applicants should have a Ph.D. degree in Cell Biology, Immunology, Biomedical Engineering, Cardiology, or in a related field, and have a strong record of publications. Preference will be given to candidates with experience in gene-editing in cells or mice, live-cell imaging, super-resolution imaging, or intravital imaging. People with inflammatory disease models in mice are encouraged to apply. Interested candidates should send their CV, representative publications, and names of three references to Dr. Zhichao Fan (email@example.com). UConn Health is an affirmative action employer, in addition to an EEO and M/F/V/PWD/PV employer.
Qualified applicants should have a Ph.D. degree in Cell Biology, Immunology, Biomedical Engineering, Cardiology, or in a related field, and have a strong record of publications. Prevalence will be given to candidates with experience in gene-editing in cells and mice, live-cell imaging, super-resolution imaging, or intravital imaging. People with disease models in mice, such as myocardial ischemia-reperfusion injury, are encouraged to apply. Interested candidates should send their CV, representative publications and names of three references to Dr. Zhichao Fan (firstname.lastname@example.org).
Accepting Lab Rotation Students: Summer 2022, Fall 2022, and Spring 2023
What will you learn?
1. Integrin activation and functions
2. Leukocyte rolling, arrest, and migration
3. Leukocyte functions in inflammatory diseases, including heart attack, cystic fibrosis, Charcot-Marie-Tooth Disease Type 2A, Cherubism, Peritonitis, and Psoriasis.
1. Cell culture
2. In silicon microfluidic device
3. Fluorescence microscopy
4. Super-resolution microscopy
5. Intravital microscopy
6. Flow cytometry (time-lapse, high-throughput)
1. Grant/fellowship writing
2. Paper writing
3. Conference opportunities
|Title or Abstract||Type||Sponsor/Event||Date/Year||Location|
|Mitofusin-2 regulates leukocyte adhesion through the maturation of beta2 integrin activation in differentiation||Talk||IMMUNOLOGY2021||2021||Virtual|
|Mitofusin-2 regulates leukocyte adhesion through the maturation of ß2 integrin activation in differentiation||Poster||EXPERIMENTAL BIOLOGY 2021||2021||Virtual|
|Nexinhib 20 Prevents Myocardial Ischemia-reperfusion Injury By Inhibiting Neutrophil Adhesion And Beta2 Integrin Activation||Poster||Basic Cardiovascular Sciences 2021||2021||Virtual|
|Mitofusin-2 regulates Neutrophil Adhesion, ß2 Integrin Activation, and Development||Talk||East Tennessee State University||2021||Johnson City, Tennessee|
|Roles of Mitofusin-2 in Neutrophil Adhesion and ß2 Integrin Activation||Talk||Clinical Research Center, UConn Health||2021||Virtual|
|Integrin Activation and Neutrophil Adhesion under Microscopes||Talk||Department of Cell Biology, UConn Health||2020||Farmington, Connecticut|
|Integrin Activation and Neutrophil Adhesion under Microscopes||Talk||Center for Cell Analysis and Modeling, UConn Health||2020||Virtual|
|Mitofusin-2 regulates leukocyte adhesion through the maturation of ß2 integrin activation in differentiation||Poster||Cell Bio Virtual 2020||2020||Virtual|
|Auto-inhibitory integrin activation during human neutrophil arrest||Talk||Immuno-Cardio Group, UConn Health||2019||Farmington, Connecticut|
|Kindlin-3 organizes a ring of clustered high affinity ß2 integrins during human neutrophil spreading under flow||Poster||Society for Leukocyte Biology||2019||Boston, Massachusetts|