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David Han, Ph.D.Associate Professor, Department of Cell BiologyCenter for Vascular Biology Director, Graduate Program in Cell Biology
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| Degree | Institution | Major |
|---|---|---|
| B.S. | Western Illinois University | Biology |
| Ph.D. | George Washington University | Genetics |
We are currently working on three areas: The first project deals with the process of recognition and engulfment of apoptotic cells. In terms of the basic biology of this process, we are interested in identification of ligands that coat apoptotic cells, receptors that recognize these ligands, signaling in apoptotic cells that mediate externalization of the ligands, and signaling in engulfing cells that mediate internalization and digestion of apoptotic cells. To address the pathophysiological consequences of this process in vivo, we are interested in developing and characterizing animal models in which ligands and receptor genes have been deleted. This project is currently funded by the American Heart Association. We are seeking additional funding from NIH to support this project. The second project deals with pluripotency of human embryonic stem cells and iPS cells. Using a phosphoproteomic approach, we are interested in identification of signaling pathways that are operational in pluripotent stem cells and changes in these cells when they begin to assume differentiated phenotype. This project is currently funded by the Connecticut State Stem Cell Initiative. We are seeking additional funding from NIH to support this project. The third project deals with biomarker discovery in human diseases such as cancer, cardiovascular disease, and multiple sclerosis. A number of proteomic studies in human disease tissues have been carried out with the overall goals to (1) identify biomarkers of human diseases, (2) uncover mechanisms of pathogenesis, and (3) provide new insights to treat human diseases. We are currently seeking funding from NIH and other sources to support these projects.
Not Accepting Students for Lab Rotations at This Time
Journal Articles
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Extracellular vesicle fibrinogen induces encephalitogenic CD8+ T cells in a mouse model of multiple sclerosis.
Proceedings of the National Academy of Sciences of the United States of America 2019 May;116(21):10488-10493
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Discovery of breast putative cancer antigens using an integrative platform of genomics-driven immunoproteomics.
Proteomics 2017 Jun;17
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Integrative proteomics, genomics, and translational immunology approaches reveal mutated forms of Proteolipid Protein 1 (PLP1) and mutant-specific immune response in multiple sclerosis.
Proteomics 2017 Feb;17
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Mapping the Interactome of a Major Mammalian Endoplasmic Reticulum Heat Shock Protein 90.
PloS one 2017 Jan;12(1):e0169260
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A draft map of rhesus monkey tissue proteome for biomedical research.
PloS one 2015 Jan;10(5):e0126243
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Large-scale proteomic characterization of melanoma expressed proteins reveals nestin and vimentin as biomarkers that can potentially distinguish melanoma subtypes.
Journal of proteome research 2014 Nov;13(11):5031-40
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Tyrosine phosphorylation of CD13 regulates inflammatory cell-cell adhesion and monocyte trafficking.
Journal of immunology (Baltimore, Md. : 1950) 2013 Aug;191(7):3905-12
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LC-MS/MS Identification of a Bromelain Peptide Biomarker from Ananas comosus Merr.
Evidence-based complementary and alternative medicine : eCAM 2012 Jan;2012548486
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Engagement of S1P₁-degradative mechanisms leads to vascular leak in mice.
The Journal of clinical investigation 2011 Jun;121(6):2290-300
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Sphingosine interaction with acidic leucine-rich nuclear phosphoprotein-32A (ANP32A) regulates PP2A activity and cyclooxygenase (COX)-2 expression in human endothelial cells.
The Journal of biological chemistry 2010 Aug;285(35):26825-31
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Differential protein expression profiles in estrogen receptor-positive and -negative breast cancer tissues using label-free quantitative proteomics.
Genes & cancer 2010 Mar;1(3):251-71
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Folding of Toll-like receptors by the HSP90 paralogue gp96 requires a substrate-specific cochaperone.
Nature communications 2010 Jan;1(79 ):79
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Human myelin proteome and comparative analysis with mouse myelin.
Proceedings of the National Academy of Sciences of the United States of America 2009 Aug;106(34):14605-10
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Pathogenic huntingtin inhibits fast axonal transport by activating JNK3 and phosphorylating kinesin.
Nature neuroscience 2009 Jul;12(7):864-71
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Identification of novel tumor antigens with patient-derived immune-selected antibodies.
Cancer immunology, immunotherapy : CII 2009 Feb;58(2):221-34
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Large-scale quantitative phosphoproteomic analysis of T Cell Receptor Signaling: System-wide modulation of protein-protein interaction mediated by site-specific phosphorylation.
Science Signaling 2009 Jan;18(2(84)):ra46
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Proteomics Analysis Reveal Unexpected Intersection of Coagulation Cascade and Immune System in Multiple Sclerosis.
Nature 2008 Jan;451(1076):1083
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Global survey of human T leukemic cells by integrating proteomics and transcriptomics profiling.
Mol & Cell Proteomics 2007 Jan;6(8):1343-1353
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Overcoming the dynamic range problem in mass spectrometry-based shotgun proteomics.
Expert review of proteomics 2006 Dec;3(6):611-9
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Absolute Quantification of Multi-site Phosphorylation by Selective Reaction Monitoring Mass Spectrometry: Determination of Inhibitory Phosphorylation Status of Cyclin Dependent Kinases.
Mol & Cell Proteomics 2006 Jan;51146-1157
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Systematic Characterization of Nuclear Proteome from Human T Leukemia Cells: A Quantitative Proteomic Study during Apoptosis by Differential Extraction and Stable Isotope Labeling.
Mol & Cell Proteomics 2006 Jan;51131-1145
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Mass spectrometry-based expression profiling of clinical prostate cancer.
Molecular & cellular proteomics : MCP 2005 Apr;4(4):545-54
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A Systematic Characterization of Mitochondrial Proteome from Human T Leukemia Cells.
Mol & Cell Proteomics 2005 Jan;4169-181
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Systematic comparison of a two-dimensional ion trap and a three-dimensional ion trap mass spectrometer in proteomics.
Molecular & cellular proteomics : MCP 2005 Jan;4(2):214-23
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Sphingosine 1-phosphate receptor regulation of N-cadherin mediates vascular stabilization.
Genes & development 2004 Oct;18(19):2392-403
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Proteomic mapping provides powerful insights into functional myelin biology.
Proceedings of the National Academy of Sciences of the United States of America 2004 Mar;101(13):4643-8
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Annexin I is an endogenous ligand that mediates apoptotic cell engulfment.
Developmental cell 2003 Apr;4(4):587-98
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A novel engulfment ligand from apoptotic cells identified by quantitative proteomics
Developmental Cell 2003 Jan;4(4):587-598
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Quantitative profiling of differentiation-induced microsomal proteins using isotope-coded affinity tags and mass spectrometry.
Nature biotechnology 2001 Oct;19(10):946-51
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Fas/FADD-mediated apoptosis triggers up-regulation of pro-inflammatory genes in vascular smooth muscle cells, followed by recruitment of macrophages in vivo.
Nature Medicine 2000 Jan;6(7):790-796
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Astrocytic TIMP-1 regulates production of Anastellin, a novel inhibitor of oligodendrocyte differentiation and FTY720 responses
bioRxiv.
Book Chapters
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Genomics-Driven Immunoproteomics: An Integrative Platform to Uncover Important Biomarkers for Human Diseases.
Methods in molecular biology (Clifton, N.J.) 2019 Jan;327-332
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Proteomics Analysis of Myelin Composition.
Methods in molecular biology (Clifton, N.J.) 2018 Jan;67-77
Abstracts
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Mass spectrometry-based identification of proteins in archival malignant melanoma tissue samples
Mod Pathol 2010 Jan;23113A
Letters
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Proteomic analysis of formalin-fixed, paraffin-embedded melanoma.
Journal of cutaneous pathology 2012 Apr;39(4):464-6
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Combined mass spectrometry- and immunohistochemistry-based approach to determine protein expression in archival melanoma--proof of principle.
Pigment cell & melanoma research 2010 Dec;23(6):849-52
Other
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Methods for Treating Breast Cancer and for Identifying Breast Cancer Antigens -U.S. Application No.: 15/990,172
US Patent 2022 Jun;
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Methods for treating breast cancer and for identifying breast cancer antigens
US Patent 11,300,574 2022 2022 Apr;
| Title or Abstract | Type | Sponsor/Event | Date/Year | Location |
|---|---|---|---|---|
| Patient-Specific Precision Antibodies Against Breast Cancer | Talk | 2018 | E-1-1036 |