Mayu Inaba, M.D., Ph.D.Assistant Professor, Department of Cell Biology
- Education & Training
- Research Opportunities
- Lab Rotations
|M.D.||Ehime University School of Medicine||Medicine|
|Ph.D.||Graduate School of Kyushu University||Pathological Medicine|
|Postdoctoral||University of Tokyo||JSPS postdoctoral research fellow|
|Postdoctoral||University of Michigan||HHMI Research Specialist I|
|Name of Award/Honor||Awarding Organization|
|R35 Outstanding Investigator Award, The National Institute of General Medical Sciences||NIH, NIGMS|
|DeLill Nasser Travel Award for Professional Development in Genetics||Genetics Society of America|
|Travel Award for 52nd Annual Meeting of the American Society for Cell Biology||American Society for Cell Biology|
|Research Fellowship 2003 - 2006||Japan Society for the Promotion of Science for Young Scientists|
MEDS-6449-Foundations of Biomedical Science II (Cell cycle)
MEDS-6497-Graduate Seminar: Cell Biology Journal Club
MEDS-5418-Stem Cells and Regenerative Biology-SECF40-1208
MEDS-5369-Advanced Genetics and Molecular Biology-SECF40-1223
MEDS-5309-Molecular Basis of Disease
Our long term goal is to understand the regulatory mechanisms of asymmetric stem cell division.
Asymmetric stem cell division is a mechanism that balances stem cell self-renewal and differentiation through the production of one stem cell and one differentiating cell. It is a simple way of maintaining the stem cell population without increasing it and is thus thought to be a vital mechanism for tissue homeostasis and tumor suppression. My research goal is to elucidate the molecular mechanism how differently fated two daughter cells are made after only one cell division. My lab primarily uses Drosophila gonads in which we can monitor asymmetric division in vivo. Owning to the simple anatomy and abundant imaging tools, this system allow us to discover previously unrecognized regulatory mechanisms and thus contribute to develop a new therapeutic approach for cancers and degenerative diseases.
Accepting Lab Rotation Students: The Drosophila germline stem cell system is an excellent model to monitor stem cell behavior while they are still located in the niche. We use a combination of approaches including CRISPR genome engineering, genetics, and quantitative and whole-mount live imaging to understand adult stem cell regulation. Results from our research are relevant to human physiology and diseases, as many of the molecules that control Drosophila stem cell system are conserved in humans.
- Understanding the flexibility of niche-stem cell interaction: Tissue stem cells are under dynamic state that is largely governed by their microenvironment, or the niche, which facilitates plasticity and adaptability of stem cells to changing conditions. The aim of 1st rotation project is to investigate dynamics of cell signaling and downstream pathway activation in Drosophila germline stem cells, while they are adapting to various physiological and pathological conditions, including dietary changes, abnormalities in nervous system, methabolic disorders and aging.
- Visualizing chromosome interaction and gene expression change in stem cell systems: Dysregulation in stem-cell gene transcriptional program leads to abberent tissue homeostasis, and then human diseases such as cancer. Growing number of evidences suggest that diversification of regulatory elements is a major source of phenotypic polymorphism among population. These DNA elements interact not only with transcription factors and other regulatory proteins, but also with other DNA elements. Our recent study identified that interhomolog interaction of stem cell gene locus is uniquely regulated and is essential for prompt downregulation of the transcript. While biological importance of interchromosomal interaction is becoming increasingly clear in recent years, very little is known about the mechanism in the control of gene expression. The 2nd rotation project is to establish the tools for monitoring homolog interaction and gene expression simutaniously, by using MS2/MCP, PP7/PCP based visualization and quantification of nascent transcripts.
Interchromosomal interaction of homologous Stat92E alleles regulates transcriptional switch during stem-cell differentiation.
Nature communications 2022 Jul;13(1):3981
Drosophila CG17003/leaky (lky) is required for microtubule acetylation in early germ cells in Drosophila ovary.
PloS one 2022 Jan;17(11):e0276704
Mad dephosphorylation at the nuclear pore is essential for asymmetric stem cell division.
Proceedings of the National Academy of Sciences of the United States of America 2021 Mar;118(13):e2006786118
Self-limiting stem-cell niche signaling through degradation of a stem-cell receptor.
PLoS biology 2020 Dec;18(12):e3001003
Drosophila CG2469 Encodes a Homolog of Human CTR9 and Is Essential for Development.
G3 (Bethesda, Md.) 2016 Dec;6(12):3849-3857
Klp10A, a stem cell centrosome-enriched kinesin, balances asymmetries in Drosophila male germline stem cell division.
eLife 2016 Nov;5
Nanotubes mediate niche-stem-cell signalling in the Drosophila testis.
Nature 2015 Jul;523(7560):329-32
The polarity protein Baz forms a platform for the centrosome orientation during asymmetric stem cell division in the Drosophila male germline.
eLife 2015 Mar;4
Piwi is required in multiple cell types to control germline stem cell lineage development in the Drosophila ovary.
PloS one 2014 Jan;9(3):e90267
Lineage tracing quantification reveals symmetric stem cell division in Drosophila male germline stem cells.
Cellular and molecular bioengineering 2013 Dec;6(4):441-448
Centrosome misorientation mediates slowing of the cell cycle under limited nutrient conditions in Drosophila male germline stem cells.
Molecular biology of the cell 2012 Apr;23(8):1524-32
String (Cdc25) regulates stem cell maintenance, proliferation and aging in Drosophila testis.
Development (Cambridge, England) 2011 Dec;138(23):5079-86
E-cadherin is required for centrosome and spindle orientation in Drosophila male germline stem cells.
PloS one 2010 Aug;5(8):e12473
Modulation of TLR4 signaling by a novel adaptor protein signal-transducing adaptor protein-2 in macrophages.
Journal of immunology (Baltimore, Md. : 1950) 2006 Jan;176(1):380-9
Selective activation of STAT5 unveils its role in stem cell self-renewal in normal and leukemic hematopoiesis.
The Journal of experimental medicine 2005 Jul;202(1):169-79
Physical and functional interactions between STAP-2/BKS and STAT5.
The Journal of biological chemistry 2005 Mar;280(9):8188-96
Regulation of FcepsilonRI-mediated signaling by an adaptor protein STAP-2/BSK in rat basophilic leukemia RBL-2H3 cells.
Biochemical and biophysical research communications 2003 Jul;306(3):767-73
STAP-2/BKS, an adaptor/docking protein, modulates STAT3 activation in acute-phase response through its YXXQ motif.
The Journal of biological chemistry 2003 Mar;278(13):11182-9
Differential control of the NIMA-related kinases, Nek6 and Nek7, by serum stimulation.
Biochemical and biophysical research communications 2003 Feb;301(4):899-906
CIS1 interacts with the Y532 of the prolactin receptor and suppresses prolactin-dependent STAT5 activation.
Journal of biochemistry 2003 Jan;133(1):109-13
The SOCS box of SOCS-1 accelerates ubiquitin-dependent proteolysis of TEL-JAK2.
The Journal of biological chemistry 2001 Apr;276(16):12530-8
Identification of a stimulus-dependent DNase I hypersensitive site between the Ialpha and Calpha exons during immunoglobulin heavy chain class switch recombination.
FEBS letters 2000 Feb;467(2-3):268-72
Ultrastructural Analysis of Cell-Cell Interactions in Drosophila Ovary.
Methods in molecular biology (Clifton, N.J.) 2021 Jan;234679-90
Evaluation of the Asymmetric Division of Drosophila Male Germline Stem Cells.
Methods in molecular biology (Clifton, N.J.) 2017 Jan;49-62
Seminars in cell & developmental biology 2022 Sep;12961-62
Removal of cellular protrusions.
Seminars in cell & developmental biology,129:126-134. 2022 Mar;
Modulation of Cell-Cell Interactions in Drosophila Oocyte Development.
Cells 2020 Jan;9(2):
Specialized Intercellular Communications via Cytonemes and Nanotubes.
Annual review of cell and developmental biology 2018 Aug;3459-84
Keeping stem cells under control: New insights into the mechanisms that limit niche-stem cell signaling within the reproductive system.
Molecular reproduction and development 2016 Aug;83(8):675-83
Signaling by Cellular Protrusions: Keeping the Conversation Private.
Trends in cell biology 2016 Jul;26(7):526-34
Asymmetric stem cell division: precision for robustness.
Cell stem cell 2012 Oct;11(4):461-9
|Title or Abstract||Type||Sponsor/Event||Date/Year||Location|
|ERATO International Symposium Kurumizaka Chromatin Atlas (Poster: Mayu Inaba)||Poster||ERATO/Tokyo University||2023||Online|
|NIH/NICHD Seminar (Talk: Mayu Inaba)||Talk||NIH/NICHD||2023||Bethesda, Maryland|
|Frontiers in Biology, Stanford University (Talk: Mayu Inaba)||Talk||Stanford University||2023||Palo Alto, San Francisco|
|Johns Hopkins University, Department of Biology Seminar Series (Talk: Mayu Inaba)||Talk||JHU||2022||Online|
|FASEB 2022, The TGF-ß Superfamily Conference: Signaling in Development and Disease (Talk: Mayu Inaba)||Talk||FASEB||2022||Malahide, Ireland|
|Developmental Biology Seminar Series, Gurdon Institute, University of Cambridge (Talk: Mayu Inaba)||Talk||Gurdon Institute, University of Cambridge||2021||Online|
|Queens College Biology Colloquium, Queens College, CUNY (Talk: Mayu Inaba)||Talk||Queens College, CUNY, NY||2019||NY, United States|
|The Fifth International Oocyte Meeting (Talk: Mayu Inaba)||Talk||The Fifth International Oocyte Meeting||2019||Nice, France|
|2019 Gordon Research Conference on Fertilization and Activation of Development (Talk: Mayu Inaba)||Talk||Gordon Research Conference||2019||NH, United States|
|Cold Spring Harbor Meeting, Germ Cells, Cold Spring Harbor Laboratory (Talk: Mayu Inaba)||Talk||Cold Spring Harbor Laboratory||2018||NY, United States|
|The 15th Stem Cell Research Symposium (Talk: Mayu Inaba)||Talk||2017||Tokyo, Japan|
|Keio University, Department of Microbiology and Immunology (Talk: Mayu Inaba)||Talk||2017||Tokyo, Japan|
|Northeast Society for Developmental Biology Regional Meeting at MBL (Talk: Mayu Inaba)||Talk||Northeast Society for Developmental Biology||2017||MA, United States|
|International Society for Stem Cell Research, Annual Meeting (Talk: Mayu Inaba)||Talk||ISSCR||2016||San Francisco, CA, United States|
|56th Annual Drosophila Research Conference, “Cell Biology & Signal Transduction” (Talk: Mayu Inaba)||Talk||GSA||2015||Chicago, IL, United States|
|Annual Meeting, The American Society for Cell Biology, “Positioning the Microtubule Organizing Center: A Matter of Life or Death?” (Talk: Mayu Inaba)||Talk||ASCB||2014||Philadelphia, PA, United States|
|52nd Annual Meeting, The American Society for Cell Biology, Mini symposium, Cell Polarity (Talk: Mayu Inaba)||Talk||ASCB||2012||San Francisco, CA, United States|