Abhijit Rath, Ph.D.Instructor of Molecular Oncology
Facilitating CRISPR based functional genomics approaches, Modulation of DNA double-strand break repair to enable precision gene editing, Investigating mechanistic basis of mismatch repair deficiency, Genetic basis of early-onset colorectal cancer
|B.VSc. & A.H.||Orissa University of Agriculture & Technology||Veterinary Science and Animal Husbandry|
|Ph.D.||LSU Health Shreveport||Biochemistry and Molecular Biology|
|Postdoctoral||UConn School of Medicine||Genetic Predisposition to Colorectal Cancer|
|Name of Award/Honor||Awarding Organization|
|Full Scholarship for the “Workshop on 3D Genome Mapping Technology-ChIA-PET”||The Jackson Laboratory|
|Carroll Feist Predoctoral Research Fellowship||LSU Health Shreveport|
|Outstanding Biochemistry Graduate Student Award (Second Place)||LSU Health Shreveport|
|First Place in Ray A. Barlow Excellence in Cancer Research Award in Barlow Symposium||LSU Health Shreveport|
|Jason A. Cardelli Award for Excellence in Cancer Research||LSU Health Shreveport|
My current research focuses on modeling Lynch syndrome (LS) associated DNA mismatch repair gene variants in human embryonic stem cells using CRISPR-Cas gene editing. We aim to gather variant specific functional data to better inform the clinical diagnosis and stratification of LS patients.
Over the course of the last few years, we have optimized CRISPR based genome engineering to serve as a highly efficient tool to site-specifically incorporate genomic single nucleotide variant change. We aim to improve the throughput of this powerful reverse genetic approach for large scale functional genomics studies and lessen the existing technical constraints. Specifically, we aim to explore novel methodologies to improve homology directed repair outcomes for CRISPR-Cas induced DNA double-strand breaks.
We also aim to study the putative genetic modifiers of mismatch repair by modeling them in human cells and functionally evalutate them for preservation of mismatch repair function.
Loss of mismatch repair promotes a direct selective advantage in human stem cells.
Stem Cell Reports 2022 Dec;17P2661-2673
A calibrated cell-based functional assay to aid classification of MLH1 DNA mismatch repair gene variants.
Human Mutation (Editor's choice) 2022 Dec;43(12):2295-2307
Enhanced CRISPR-based DNA demethylation by Casilio-ME-mediated RNA-guided coupling of methylcytosine oxidation and DNA repair pathways.
Nature communications 2019 Sep;10(1):4296
Functional Interrogation of Lynch Syndrome Associated MSH2 Missense Variants via CRISPR-Cas9 Gene Editing in Human Embryonic Stem Cells.
Human mutation (Editor's choice) 2019 Jun;
Fidelity of end joining in mammalian episomes and the impact of Metnase on joint processing.
BMC molecular biology 2014 Mar;156
Phenothiazine Inhibitors of TLKs Affect Double-Strand Break Repair and DNA Damage Response Recovery and Potentiate Tumor Killing with Radiomimetic Therapy.
Genes & cancer 2013 Jan;4(1-2):39-53
|Title or Abstract||Type||Sponsor/Event||Date/Year||Location|
|A calibrated cell-based functional assay to aide classification of MLH1 DNA mismatch repair gene variants||Talk||The International Society for Gastrointestinal Hereditary Tumors (InSiGHT)||2022||New Jersey|