David W. Rowe, MDDirector, Center for Regenerative Medicine and Skeletal Development
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Education
Degree | Institution | Major |
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BA | University of Vermont | Chemistry |
MD | University of Vermont | Medicine |
The primary focus of the laboratory is to recognize and ultimately isolate subpopulations of cells at define levels of differentiation within the skeletal lineage for bone, articular cartilage, temporal mandibular cartilage and tendon. This goal has been made possible by the construction of a series of mice transgenic for promoter-GFP reporter genes that activate at defined levels of differentiation. Fluorescent microscopic methods have been developed to observed lineage progression in real time in culture and in adult skeletal tissues with specific reference to murine models of skeletal tissue repair. The goal is to map where a mutation acts to impede forward progress of cell development and to correlate its in vivo impact within intact mouse bone. In addition, the models for evaluating progenitor activity have been adapted to human derived cells from either adult or hES/iPS sources. Particular attention is directed in multiplexing various colors of GFP and fluorescent cellular stains to maximize the information available within a histological section of mineralized tissues. We participate regularly with members of the Computer Science department at Storrs to develop advanced image analysis of skeletal images and new approaches for interpreting microarray and chromatin immunoprecipitation studies.
Current projects that utilize this experimental approach include: a) microarray analysis of genes expressed in FAC isolated subpopulations within the osteoblast, chondrogenic and tendon lineage; b) development of models of skeletal repair to test the progenitor potential of various stem cell sources and scaffolds used to contain the cell within the defect zone; c) development of efficient fluorescent imaging techniques for skeletal tissues and application of image analysis techniques for automating incremental differences in skeletal repair; d) animal models of osteogenesis imperfecta to evaluate strategies of gene correction and cell replacement. Graduate students will be exposed to techniques of DNA cloning and BAC recombineering, cell culture of primary progenitor cells from adult animals and ES/iPS cells from human sources, hybridization and NGS sequence based microarray analysis of RNA, histological assessment of the osteoblast lineage and management and analysis of murine models of human disease including skeletal repair and tissue engineering.
Current projects that utilize this experimental approach include: a) microarray analysis of genes expressed in FAC isolated subpopulations within the osteoblast, chondrogenic and tendon lineage; b) development of models of skeletal repair to test the progenitor potential of various stem cell sources and scaffolds used to contain the cell within the defect zone; c) development of efficient fluorescent imaging techniques for skeletal tissues and application of image analysis techniques for automating incremental differences in skeletal repair; d) animal models of osteogenesis imperfecta to evaluate strategies of gene correction and cell replacement. Graduate students will be exposed to techniques of DNA cloning and BAC recombineering, cell culture of primary progenitor cells from adult animals and ES/iPS cells from human sources, hybridization and NGS sequence based microarray analysis of RNA, histological assessment of the osteoblast lineage and management and analysis of murine models of human disease including skeletal repair and tissue engineering.
Not accepting students for Lab Rotations at this time
Journal Articles
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Modeling of intramembranous ossification using human pluripotent stem cell-derived paraxial mesoderm derivatives.
Regenerative therapy 2023 Dec;24536-546
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Stem cell-based modeling and single-cell multiomics reveal gene-regulatory mechanisms underlying human skeletal development.
Cell reports 2023 Mar;112276
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Ultrasound-derived mechanical stimulation of cell-laden collagen hydrogels for bone repair.
Journal of biomedical materials research. Part A 2023 Feb;
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Predicting the targets of IRF8 and NFATc1 during osteoclast differentiation using the machine learning method framework cTAP.
BMC genomics 2022 Jan;23(1):14
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Parental Origin of Gsα Inactivation Differentially Affects Bone Remodeling in a Mouse Model of Albright Hereditary Osteodystrophy.
JBMR plus 2022 Jan;6(1):e10570
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Senolytics improve bone forming potential of bone marrow mesenchymal stem cells from aged mice.
NPJ Regenerative medicine 2021 Jun;6(1):34
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Mesenchyme-specific loss of Dot1L histone methyltransferase leads to skeletal dysplasia phenotype in mice.
Bone 2021 Jan;142115677
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Effects of acidosis on the structure, composition, and function of adult murine femurs.
Acta biomaterialia 2020 Nov;
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Skeletal Screening IMPC/KOMP using μCT and Computer Automated Cryohistology: Application to the Efna4 KO mouse line.
Bone 2020 Oct;115688
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Stepwise strategy for generating osteoblasts from human pluripotent stem cells under fully defined xeno-free conditions with small-molecule inducers.
Regenerative therapy 2020 Jun;1419-31
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Intrafibrillar Mineralized Collagen-Hydroxyapatite-Based Scaffolds for Bone Regeneration.
ACS applied materials & interfaces 2020 Apr;12(16):18235-18249
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Transplanting cells from old but not young donors causes physical dysfunction in older recipients.
Aging Cell. 2020 Jan;e13106
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Biodegradable nanofiber-based piezoelectric transducer.
Proceedings of the National Academy of Sciences of the United States of America 2020 Jan;
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American Society for Bone and Mineral Research-Orthopaedic Research Society Joint Task Force Report on Cell-Based Therapies.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 2019 Sep;
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Parenchymal and stromal tissue regeneration of tooth organ by pivotal signals reinstated in decellularized matrix.
Nature materials 2019 Jun;18(6):627-637
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Evaluation of an Engineered Hybrid Matrix for Bone Regeneration via Endochondral Ossification.
Annals of biomedical engineering 2019 Apr;
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Histological Criteria that Distinguish Human and Mouse Bone Formed Within a Mouse Skeletal Repair Defect.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2019 Mar;22155419836436
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Spatially controlled rhBMP-2 mediated calvarial bone formation in a transgenic mouse model.
International journal of biological macromolecules 2018 Aug;1061159-1165
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Osteochondral Differentiation of Fluorescent Multi-Reporter Cells on Zonally-Organized Biomaterials.
Tissue engineering. Part A 2018 Aug;
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Continuing Challenges in Advancing Preclinical Science in Skeletal Cell-Based Therapies and Tissue Regeneration.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 2018 Aug;331721-1728
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Dickkopf-3 in aberrant endothelial secretome triggers renal fibroblast activation and endothelial-mesenchymal transition.
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association 2018 May;3449-62
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Screening Gene Knockout Mice for Variation in Bone Mass: Analysis by μCT and Histomorphometry.
Current osteoporosis reports 2018 Apr;16(2):77-94
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Laser-Capture Microdissection and RNA Extraction from Perfusion-Fixed Cartilage and Bone Tissue from Mice Implanted with Human iPSC-Derived MSCs in a Calvarial Defect Model.
Methods in molecular biology (Clifton, N.J.) 2018 Jan;1723385-396
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Cell origin, volume and arrangement are drivers of articular cartilage formation, morphogenesis and response to injury in mouse limbs.
Developmental biology 2017 Apr;42656-68
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Variable patterns of ectopic mineralization in Enpp1asj-2J mice, a model for generalized arterial calcification of infancy.
Oncotarget 2016 Nov;783837-83842
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The LG/J Murine Strain Exhibits Near-Normal Tendon Biomechanical Properties Following a Full-Length Central Patellar Tendon Defect.
Connective tissue research 2016 Aug;57496-506
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Quiescent Bone Lining Cells Are a Major Source of Osteoblasts During Adulthood.
Stem cells (Dayton, Ohio) 2016 Aug;342930-2942
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Murine supraspinatus tendon injury model to identify the cellular origins of rotator cuff healing.
Connective tissue research 2016 May;57507-515
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Anabolic actions of Notch on mature bone.
Proceedings of the National Academy of Sciences of the United States of America 2016 Mar;113E2152-61
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Ectopic mineralization of cartilage and collagen-rich tendons and ligaments in Enpp1asj-2J mice.
Oncotarget 2016 Feb;712000-9
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Evaluation of the donor cell contribution in rhBMP-2 mediated bone formation with chitosan thermogels using fluorescent protein reporter mice.
Journal of biomedical materials research. Part A 2015 Dec;104928-41
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Whole-genome sequencing identifies EN1 as a determinant of bone density and fracture.
Nature 2015 Oct;526(7571):112-7
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Serine dipeptide lipids of Porphyromonas gingivalis inhibit osteoblast differentiation: Relationship to Toll-like receptor 2.
Bone 2015 Sep;81654-61
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Identification of a progenitor cell population destined to form fracture fibrocartilage callus in Dickkopf-related protein 3-green fluorescent protein reporter mice.
Journal of bone and mineral metabolism 2015 Sep;34606-614
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Cell and matrix response of temporomandibular cartilage to mechanical loading.
Osteoarthritis and cartilage / OARS, Osteoarthritis Research Society 2015 Sep;24335-44
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Improving the permeability of lyophilized collagen-hydroxyapatite scaffolds for cell-based bone regeneration with a gelatin porogen.
Journal of biomedical materials research. Part B, Applied biomaterials 2015 Aug;1041580-1590
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Ablating hedgehog signaling in tenocytes during development impairs biomechanics and matrix organization of the adult murine patellar tendon enthesis.
Journal of orthopaedic research : official publication of the Orthopaedic Research Society 2015 Aug;33(8):1142-51
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Fixation stability dictates the differentiation pathway of periosteal progenitor cells in fracture repair.
Journal of orthopaedic research : official publication of the Orthopaedic Research Society 2015 Jul;33(7):948-56
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Gdf5 progenitors give rise to fibrocartilage cells that mineralize via hedgehog signaling to form the zonal enthesis.
Developmental biology 2015 Jun;405(1):96-107
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Improved biomechanical and biological outcomes in the MRL/MpJ murine strain following a full-length patellar tendon injury.
Journal of orthopaedic research : official publication of the Orthopaedic Research Society 2015 May;33(11):1693-703
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Combination therapy with PTH and DBM cannot heal a critical sized murine femoral defect.
Journal of orthopaedic research : official publication of the Orthopaedic Research Society 2015 Apr;33(8):1242-9
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The role of transduced bone marrow cells overexpressing BMP-2 in healing critical-sized defects in a mouse femur.
Gene therapy 2015 Mar;22(6):467-75
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Response of knee fibrocartilage to joint destabilization.
Osteoarthritis and cartilage / OARS, Osteoarthritis Research Society 2015 Feb;23(6):996-1006
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Evaluation of rhBMP-2 and bone marrow derived stromal cell mediated bone regeneration using transgenic fluorescent protein reporter mice.
Journal of biomedical materials research. Part A 2014 Dec;102(12):4568-80
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Mouse limb skeletal growth and synovial joint development are coordinately enhanced by Kartogenin.
Developmental biology 2014 Sep;395(2):255-67
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A site-specific integrated Col2.3GFP reporter identifies osteoblasts within mineralized tissue formed in vivo by human embryonic stem cells.
Stem Cells Translational Medicine 2014 Aug;3(10):1125-37
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Induced ablation of Bmp1 and Tll1 produces osteogenesis imperfecta in mice.
Human molecular genetics 2014 Jun;23(12):3085-101
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Evaluation of osteogenic cell differentiation in response to bone morphogenetic protein or demineralized bone matrix in a critical sized defect model using GFP reporter mice.
Journal of orthopaedic research : official publication of the Orthopaedic Research Society 2014 Jun;32(9):1120-8
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Local transplantation is an effective method for cell delivery in the osteogenesis imperfecta murine model.
International orthopaedics 2014 Jan;38(9):1955-62
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Effects of cell-attachment and extracellular matrix on bone formation in vivo in collagen-hydroxyapatite scaffolds.
PloS one 2014 Jan;9(10):e109568
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Developmental-like bone regeneration using human embryonic stem cell-derived mesenchymal cells.
Tissue Engineering, Part A 2013 Aug;20(1-2):365-377
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Fabrication and characterization of biomimetic collagen-apatite scaffolds with tunable structures for bone tissue engineering.
Acta biomaterialia 2013 Jul;9(7):7308-19
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A novel GFP reporter mouse reveals Mustn1 expression in adult regenerating skeletal muscle, activated satellite cells and differentiating myoblasts.
Acta physiologica (Oxford, England) 2013 Jun;208(2):180-90
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Mechanical input restrains PPARγ2 expression and action to preserve mesenchymal stem cell multipotentiality.
Bone 2013 Jan;52(1):454-64
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Osterix-cre labeled progenitor cells contribute to the formation and maintenance of the bone marrow stroma.
PloS one 2013 Jan;8(8):e71318
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A Bayesian Approach to Pathway Analysis by Integrating Gene-Gene Functional Directions and Microarray Data.
Statistics in biosciences 2012 May;4(1):105-131
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In vivo fate mapping identifies mesenchymal progenitor cells.
Stem cells (Dayton, Ohio) 2012 Feb;30(2):187-96
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Controlling the structural organization of regenerated bone by tailoring tissue engineering scaffold architecture
Journal of Materials Chemistry 2012 Jan;22(19):9721-9730
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Isolation and characterization of murine mandibular condylar cartilage cell populations.
Cells, tissues, organs 2012 Jan;195(3):232-43
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Generation and characterization of Col10a1-mcherry reporter mice.
Genesis (New York, N.Y. : 2000) 2011 May;49(5):410-8
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Early effects of orthodontic forces on osteoblast differentiation in a novel mouse organ culture model.
The Angle orthodontist 2011 Mar;81(2):284-91
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Promoting information sharing for multijurisdictional public health emergency preparedness.
Journal of public health management and practice : JPHMP 2011 Jan;17(1):84-9
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Isolation of murine bone marrow derived mesenchymal stem cells using Twist2 Cre transgenic mice.
Bone 2010 Nov;47(5):916-25
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Long bone fracture repair in mice harboring GFP reporters for cells within the osteoblastic lineage.
Journal of orthopaedic research : official publication of the Orthopaedic Research Society 2010 Oct;28(10):1338-47
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Porphyromonas gingivalis lipids inhibit osteoblastic differentiation and function.
Infection and immunity 2010 Sep;78(9):3726-35
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Immature osteoblast lineage cells increase osteoclastogenesis in osteogenesis imperfecta murine.
The American journal of pathology 2010 May;176(5):2405-13
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Learning Bayesian networks with integration of indirect prior knowledge.
International journal of data mining and bioinformatics 2010 Jan;4(5):505-19
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Computing consistency between microarray data and known gene regulation relationships.
IEEE transactions on information technology in biomedicine : a publication of the IEEE Engineering in Medicine and Biology Society 2009 Nov;13(6):1075-82
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Determination of the fate and contribution of ex vivo expanded human bone marrow stem and progenitor cells for bone formation by 2.3ColGFP.
Molecular therapy : the journal of the American Society of Gene Therapy 2009 Nov;17(11):1967-78
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Identification of differentially expressed genes between osteoblasts and osteocytes.
Bone 2009 Oct;45(4):682-92
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A BAC-bacterial recombination method to generate physically linked multiple gene reporter DNA constructs.
BMC biotechnology 2009 Jan;920
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Live-animal tracking of individual haematopoietic stem/progenitor cells in their niche.
Nature 2009 Jan;457(7225):92-6
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A rapid, nonradioactive in situ hybridization technique for use on cryosectioned adult mouse bone.
Calcified tissue international 2008 Sep;83(3):212-21
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Use of an alpha-smooth muscle actin GFP reporter to identify an osteoprogenitor population.
Bone 2008 Sep;43(3):501-10
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Effects of transient PTH on early proliferation, apoptosis, and subsequent differentiation of osteoblast in primary osteoblast cultures.
American journal of physiology. Endocrinology and metabolism 2007 Feb;292(2):E594-603
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Pericyte/myofibroblast phenotype of osteoprogenitor cell.
Journal of musculoskeletal & neuronal interactions 2007 Jan;7(4):320-2
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The Wnt co-receptor LRP5 is essential for skeletal mechanotransduction but not for the anabolic bone response to parathyroid hormone treatment.
The Journal of biological chemistry 2006 Aug;281(33):23698-711
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Pathogenic role of Fgf23 in Hyp mice.
American journal of physiology. Endocrinology and metabolism 2006 Jul;291(1):E38-49
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Examination of mineralized nodule formation in living osteoblastic cultures using fluorescent dyes.
Biotechnology progress 2006 Jan;22(6):1697-701
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Heterogeneity of engrafted bone-lining cells after systemic and local transplantation.
Blood 2005 Nov;106(10):3650-7
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Origins of endothelial and osteogenic cells in the subcutaneous collagen gel implant.
Bone 2005 Nov;37(5):678-87
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Visualizing levels of osteoblast differentiation by a two-color promoter-GFP strategy: Type I collagen-GFPcyan and osteocalcin-GFPtpz.
Genesis (New York, N.Y. : 2000) 2005 Oct;43(2):87-98
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Dkk2 has a role in terminal osteoblast differentiation and mineralized matrix formation.
Nature genetics 2005 Sep;37(9):945-52
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Expression profile of osteoblast lineage at defined stages of differentiation.
The Journal of biological chemistry 2005 Jul;280(26):24618-26
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Dentin matrix protein 1 gene cis-regulation: use in osteocytes to characterize local responses to mechanical loading in vitro and in vivo.
The Journal of biological chemistry 2005 May;280(21):20680-90
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Histological analysis of GFP expression in murine bone.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2005 May;53(5):593-602
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Comparison of the action of transient and continuous PTH on primary osteoblast cultures expressing differentiation stage-specific GFP.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 2005 Jan;20(1):5-14
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Modification of human U1 snRNA for inhibition of gene expression at the level of pre-mRNA.
Methods in molecular biology (Clifton, N.J.) 2005 Jan;309321-32
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Viewing problems in bone biology from the perspective of lineage identification.
Journal of musculoskeletal & neuronal interactions 2005 Jan;5(4):350-2
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In vitro and in vivo study on osteocyte-specific mechanical signaling pathways.
Journal of musculoskeletal & neuronal interactions 2004 Dec;4(4):386-7
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Dentin matrix protein 1 expression during osteoblastic differentiation, generation of an osteocyte GFP-transgene.
Bone 2004 Jul;35(1):74-82
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Hematopoiesis is severely altered in mice with an induced osteoblast deficiency.
Blood 2004 May;103(9):3258-64
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Modified U1 snRNA suppresses expression of a targeted endogenous RNA by inhibiting polyadenylation of the transcript.
Nucleic acids research 2004 Jan;32(4):1512-7
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Dual reporter transgene driven by 2.3Col1a1 promoter is active in differentiated osteoblasts.
Croatian medical journal 2003 Aug;44(4):412-7
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Inhibiting expression of specific genes in mammalian cells with 5' end-mutated U1 small nuclear RNAs targeted to terminal exons of pre-mRNA.
Proceedings of the National Academy of Sciences of the United States of America 2003 Jul;100(14):8264-9
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Stage specific inhibition of osteoblast lineage differentiation by FGF2 and noggin.
Journal of cellular biochemistry 2003 Apr;88(6):1168-76
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An adaptive strategy for single- and multi-cluster gene assignment.
Biotechnology progress 2003 Jan;19(4):1142-8
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Analysis of inhibitory action of modified U1 snRNAs on target gene expression: discrimination of two RNA targets differing by a 1 bp mismatch.
Nucleic acids research 2002 Jun;30(11):2329-39
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Osteoblastic response to the defective matrix in the osteogenesis imperfecta murine (oim) mouse.
Endocrinology 2002 May;143(5):1594-601
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A strategy for identifying osteoporosis risk genes.
Endocrine 2002 Feb;17(1):67-75
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Col1a1-GFP transgene expression in developing incisors.
Connective tissue research 2002 Jan;43(2-3):216-9
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Use of type I collagen green fluorescent protein transgenes to identify subpopulations of cells at different stages of the osteoblast lineage.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 2002 Jan;17(1):15-25
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Conditional ablation of the osteoblast lineage in Col2.3deltatk transgenic mice.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 2001 Dec;16(12):2222-31
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Human bone marrow stromal cells are efficiently transduced by vesicular stomatitis virus-pseudotyped retrovectors without affecting subsequent osteoblastic differentiation.
Bone 2001 Oct;29(4):331-5
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Dlx5 induces expression of COL1A1 promoter contained in a retrovirus vector.
Croatian medical journal 2001 Aug;42(4):436-9
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Osteogenesis imperfecta at the beginning of bone and joint decade.
Croatian medical journal 2001 Aug;42(4):393-415
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Col1a1-driven transgenic markers of osteoblast lineage progression.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 2001 Jul;16(7):1228-36
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Use of VSV-G pseudotyped retroviral vectors to target murine osteoprogenitor cells.
Virology 2001 May;284(1):37-45
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Bone-directed expression of Col1a1 promoter-driven self-inactivating retroviral vector in bone marrow cells and transgenic mice.
Molecular therapy : the journal of the American Society of Gene Therapy 2001 Apr;3(4):543-50
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Reduction of target gene expression by a modified U1 snRNA.
Molecular and cellular biology 2001 Apr;21(8):2815-25
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Premature termination codon in the aggrecan gene of nanomelia and its influence on mRNA transport and stability.
Croatian medical journal 1999 Dec;40(4):528-32
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Neuroradiology case of the day. Spinal cord compression caused by extramedullary hematopoiesis.
AJR. American journal of roentgenology 1999 Sep;173(3):806, 810-2
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Ectopic Msx2 overexpression inhibits and Msx2 antisense stimulates calvarial osteoblast differentiation.
Developmental biology 1999 May;209(2):298-307
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Contrasting post-traumatic serial changes for D-dimer and PAI-1 in critically injured patients.
Thrombosis research 1999 May;94(3):175-85
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Receptor tyrosine kinase expression in human bone marrow stromal cells.
Journal of cellular physiology 1998 Dec;177(3):426-38
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1,25-Dihydroxyvitamin D3 inhibition of col1a1 promoter expression in calvariae from neonatal transgenic mice.
Biochimica et biophysica acta 1998 Jul;1398(3):285-93
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Interleukin-1 represses COLIA1 promoter activity in calvarial bones of transgenic ColCAT mice in vitro and in vivo.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 1998 Jul;13(7):1076-83
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COL1A1 transgene expression in stably transfected osteoblastic cells. Relative contributions of first intron, 3'-flanking sequences, and sequences derived from the body of the human COL1A1 minigene.
The Journal of biological chemistry 1997 Dec;272(50):31241-50
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Bone formation in vivo: comparison of osteogenesis by transplanted mouse and human marrow stromal fibroblasts.
Transplantation 1997 Apr;63(8):1059-69
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Identification of a TAAT-containing motif required for high level expression of the COL1A1 promoter in differentiated osteoblasts of transgenic mice.
The Journal of biological chemistry 1996 Jul;271(27):16422-9
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Infusions of lidocaine into the amygdala, but not the preoptic area, block pseudopregnancy in the rat.
Journal of neuroendocrinology 1996 Apr;8(4):259-66
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Nuclear retention of COL1A1 messenger RNA identifies null alleles causing mild osteogenesis imperfecta.
The Journal of clinical investigation 1996 Feb;97(4):1035-40
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Differential regulation of COL2A1 expression in developing and mature chondrocytes.
Matrix biology : journal of the International Society for Matrix Biology 1995 Dec;14(9):753-64
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Regulation of COL1A1 expression in type I collagen producing tissues: identification of a 49 base pair region which is required for transgene expression in bone of transgenic mice.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 1995 Oct;10(10):1443-51
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Novel use for BAL 31 nuclease-generated nested deletions: sequencing from the inside out.
BioTechniques 1995 Apr;18(4):614-7
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Regulation of type I collagen gene expression in bone.
Connective tissue research 1995 Jan;31(4):261-4
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Analysis of regulatory regions in the COL1A1 gene responsible for 1,25-dihydroxyvitamin D3-mediated transcriptional repression in osteoblastic cells.
Journal of cellular biochemistry 1994 Dec;56(4):490-501
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Measurement of interleukin-1 stimulated constitutive prostaglandin G/H synthase (cyclooxygenase) mRNA levels in osteoblastic MC3T3-E1 cells using competitive reverse transcriptase polymerase chain reaction.
Endocrine research 1994 Aug;20(3):219-33
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Molecular basis of nanomelia, a heritable chondrodystrophy of chicken.
Matrix biology : journal of the International Society for Matrix Biology 1994 Aug;14(4):297-305
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Bleomycin stimulates pro-alpha 1 (I) collagen promoter through transforming growth factor beta response element by intracellular and extracellular signaling.
The Journal of biological chemistry 1994 May;269(18):13156-61
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Regulation of the alpha 1(I) collagen promoter in vascular smooth muscle cells. Comparison with other alpha 1(I) collagen-producing cells in transgenic animals and cultured cells.
The Journal of biological chemistry 1994 Feb;269(7):4903-9
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Upstream regulatory elements necessary for expression of the rat COL1A1 promoter in transgenic mice.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 1994 Feb;9(2):285-92
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Defective splicing of mRNA from one COL1A1 allele of type I collagen in nondeforming (type I) osteogenesis imperfecta.
The Journal of clinical investigation 1993 Oct;92(4):1994-2002
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c-Fos proto-oncogene activity induced by mating in the preoptic area, hypothalamus and amygdala in the female rat: role of afferent input via the pelvic nerve.
Brain research 1993 Sep;621(1):25-34
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Transgenic expression of COL1A1-chloramphenicol acetyltransferase fusion genes in bone: differential utilization of promoter elements in vivo and in cultured cells.
Molecular and cellular biology 1993 Sep;13(9):5168-74
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[Primary management of polytrauma. Comparison of a German and American air rescue unit].
Der Unfallchirurg 1993 Jun;96(6):287-91
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A mutation of the glucocorticoid receptor in primary cortisol resistance.
The Journal of clinical investigation 1993 May;91(5):1918-25
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Defective pro alpha 2(I) collagen synthesis in a recessive mutation in mice: a model of human osteogenesis imperfecta.
Proceedings of the National Academy of Sciences of the United States of America 1993 Mar;90(5):1701-5
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Parathyroid hormone represses alpha 1(I) collagen promoter activity in cultured calvariae from neonatal transgenic mice.
Molecular endocrinology (Baltimore, Md.) 1993 Mar;7(3):399-408
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On-scene helicopter transport of patients with multiple injuries--comparison of a German and an American system.
The Journal of trauma 1992 Oct;33(4):548-53; discussion 553-5
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An osteopenic nonfracture syndrome with features of mild osteogenesis imperfecta associated with the substitution of a cysteine for glycine at triple helix position 43 in the pro alpha 1(I) chain of type I collagen.
The Journal of clinical investigation 1992 Feb;89(2):567-73
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Differential utilization of regulatory domains within the alpha 1(I) collagen promoter in osseous and fibroblastic cells.
The Journal of cell biology 1992 Jan;116(1):227-36
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Transforming-growth-factor-beta activation elements in the distal promoter regions of the rat alpha 1 type I collagen gene.
The Biochemical journal 1991 Nov;280 ( Pt 1)157-62
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Cell adhesion regulates pro-alpha 1(I) collagen mRNA stability and transcription in mouse fibroblasts.
The Journal of biological chemistry 1991 May;266(13):8470-5
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Cloning and analysis of the 5' region of the rat bone/liver/kidney/placenta alkaline phosphatase gene. A dual-function promoter.
Matrix (Stuttgart, Germany) 1990 Mar;10(1):38-47
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A glucocorticoid receptor mutation in a subject with primary cortisol resistance.
Transactions of the Association of American Physicians 1990 Jan;10353-63
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Expression of chloramphenicol acetyltransferase (CAT) from rodent type I collagen promoters in transfected osteosarcoma cells in vivo.
Matrix (Stuttgart, Germany) 1989 Nov;9(5):376-81
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Insulin increases the steady state level of alpha-1(I) procollagen mRNA in the osteoblast-rich segment of fetal rat calvaria.
Endocrinology 1989 Sep;125(3):1430-7
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1,25-Dihydroxyvitamin D3 inhibits transcription of type I collagen genes in the rat osteosarcoma cell line ROS 17/2.8.
Endocrinology 1989 Jul;125(1):327-33
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Detection of mutations in human type I collagen mRNA in osteogenesis imperfecta by indirect RNase protection.
The Journal of biological chemistry 1989 Jun;264(16):9632-7
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Isolation and characterization of the rat alpha 1(I) collagen promoter. Regulation by 1,25-dihydroxyvitamin D.
The Journal of biological chemistry 1989 Feb;264(6):3072-7
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Malignant osteoporosis and defective immunoregulation.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 1988 Oct;3(5):509-16
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Analysis of cytoplasmic and nuclear messenger RNA in fibroblasts from patients with type I osteogenesis imperfecta.
Methods in enzymology 1987 Jan;145223-35
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Hormonal regulation of collagen synthesis in a clonal rat osteosarcoma cell line.
Endocrinology 1986 Nov;119(5):1922-8
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Relationship of somatomedin-C concentration to bone age in boys with constitutional delay of growth.
American journal of diseases of children (1960) 1986 Jun;140(6):555-8
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Bleomycin selectively elevates mRNA levels for procollagen and fibronectin following acute lung injury.
Biochemical and biophysical research communications 1985 Sep;131(2):836-43
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Diminished type I collagen synthesis and reduced alpha 1(I) collagen messenger RNA in cultured fibroblasts from patients with dominantly inherited (type I) osteogenesis imperfecta.
The Journal of clinical investigation 1985 Aug;76(2):604-11
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Radioimmunoassay for type I procollagen in growth hormone-deficient children before and during treatment with growth hormone.
Pediatric research 1985 Jan;19(1):8-11
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Construction of DNA sequences complementary to rat alpha 1 and alpha 2 collagen mRNA and their use in studying the regulation of type I collagen synthesis by 1,25-dihydroxyvitamin D.
Biochemistry 1984 Dec;23(25):6210-6
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Two-dimensional echocardiography in double orifice mitral valve.
Journal of the American College of Cardiology 1984 Aug;4(2):429-33
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Imperfect osteogenesis and osteoporosis.
The New England journal of medicine 1984 Jun;310(26):1738-40
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Hypophosphatemia and hypercalciuria in small premature infants fed human milk: evidence for inadequate dietary phosphorus.
The Journal of pediatrics 1984 Jan;104(1):112-7
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Cellular zinc accumulation in anencephaly and spina bifida.
Zeitschrift für Kinderchirurgie : organ der Deutschen, der Schweizerischen und der Osterreichischen Gesellschaft für Kinderchirurgie = Surgery in infancy and childhood 1983 Dec;38 Suppl 2(SUPPL. 2):65-7
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Osteogenesis imperfecta and Paget's disease of bone. Biochemical and morphologic studies.
Archives of internal medicine 1983 Dec;143(12):2250-7
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Collagen genes and brittle bones.
Annals of internal medicine 1983 Nov;99(5):700-4
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Abnormal alpha 2-chain in type I collagen from a patient with a form of osteogenesis imperfecta.
The Journal of clinical investigation 1983 Mar;71(3):689-97
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Left ventricular performance during exercise in patients with left bundle branch block: evaluation by gated radionuclide ventriculography.
American heart journal 1983 Jan;105(1):66-71
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The noninvasive diagnosis of coronary artery disease in patients with left bundle-branch block.
Texas Heart Institute journal / from the Texas Heart Institute of St. Luke's Episcopal Hospital, Texas Children's Hospital 1982 Dec;9(4):397-406
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Regulation of collagen synthesis in fetal rat calvaria by 1,25-dihydroxyvitamin D3.
The Journal of biological chemistry 1982 Jul;257(14):8009-15
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Hearing and middle ear function in osteogenesis imperfecta.
JAMA 1982 Apr;247(15):2120-6
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Initial diastolic indentation of the mitral valve in aortic insufficiency.
Journal of clinical ultrasound : JCU 1982 Feb;10(2):53-7
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Marfan syndrome: abnormal alpha 2 chain in type I collagen.
Proceedings of the National Academy of Sciences of the United States of America 1981 Dec;78(12):7745-9
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Collagen and collagen-derived fragments are chemotactic for tumor cells.
The Journal of clinical investigation 1981 Oct;68(4):1102-5
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Left ventricular volumes and ejection fraction derived from apical two-dimensional echocardiography.
Cardiovascular diseases 1981 Sep;8(3):344-354
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The value of electrocardiographic R-wave changes in exercise testing: Preexercise versus postexercise measurements.
Cardiovascular diseases 1981 Sep;8(3):333-343
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Technique for evaluating left ventricular performance with apical two-dimensional echocardiography: Progress report.
Cardiovascular diseases 1981 Mar;8(1):39-47
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Parathyroid hormone alters collagen synthesis and procollagen mRNA levels in fetal rat calvaria.
Proceedings of the National Academy of Sciences of the United States of America 1980 Oct;77(10):5654-8
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Hypophosphatemic rickets and breast milk.
The New England journal of medicine 1979 Jun;300(24):1397-8
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Regulation of procollagen synthesis during the development of chick embryo calvaria. Correlation with procollagen mRNA content.
The Journal of biological chemistry 1979 May;254(9):3526-30
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NH2-terminal sequence of the chick proalpha1(I) chain synthesized in the reticulocyte lysate system. Evidence for a transient hydrophobic leader sequence.
The Journal of biological chemistry 1979 Mar;254(5):1433-6
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The effect of amphotericin B-deoxycholate on proliferation and protein synthesis in human skin fibroblast cultures.
In vitro 1978 Dec;14(12):1003-9
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Inhibitory effects of glucocorticoids on collagen synthesis by mouse sponge granulomas and granuloma fibroblasts in culture.
Biochimica et biophysica acta 1978 Apr;540(1):101-16
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Effects of component length and of the transitions among components in multiple schedules.
Journal of the experimental analysis of behavior 1978 Jan;29(1):3-16
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Possible complications of the levodopa-propranolol test.
Pediatrics 1977 Jul;60(1):132-3
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Abnormalities in proliferation and protein synthesis in skin fibroblast cultures from patients with diabetes mellitus.
Diabetes 1977 Apr;26(4):284-90
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Decreased lysyl oxidase activity in the aneurysm-prone, mottled mouse.
The Journal of biological chemistry 1977 Feb;252(3):939-42
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Differences in growth response to hydrocortisone and ascorbic acid by human diploid fibroblasts.
In vitro 1977 Jan;13(12):824-30
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Patients with Ehlers-Danlos syndrome type IV lack type III collagen.
Proceedings of the National Academy of Sciences of the United States of America 1975 Apr;72(4):1314-6
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The molecular bases of certain inherited diseases of connective tissues involving collagen.
Birth defects original article series 1975 Jan;11(6):11-3
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The effect of cadmium on population growth of the green alga. Scenedesmus quadracauda.
Bulletin of environmental contamination and toxicology 1974 Oct;12(4):442-5
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Cadmium uptake and time dependent alterations in tissue levels in the white catfish Ictalurus catus (Pisces:Ictaluridae).
Bulletin of environmental contamination and toxicology 1974 Mar;11(3):244-9
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A sex-linked defect in the cross-linking of collagen and elastin associated with the mottled locus in mice.
The Journal of experimental medicine 1974 Jan;139(1):180-92
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Acquired and heritable defects in collagen synthesis and fibrogenesis.
Transactions of the American Clinical and Climatological Association 1974 Jan;85130-41
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Solid phase radioimmunoassay of properdin.
Immunochemistry 1973 May;10(5):341-50
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Experimental obesity in man.
Transactions of the Association of American Physicians 1968 Jan;81153-70
Abstracts
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Fluorescent quantification of osteogenesis using GFP-transgenic mouse reporter cell technology.
Regenerative Medicine-Advancing Next Generation Therapies 2009 Jan;
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Quantitation of constitutive prostaglandin G/H synthase (cyclooxygenase) mRNA levels in mouse osteo-blastic MC3T3 E1 cells using competitive polymerase chain reaction.
J Bone and Miner Res 1993 Jan;8(S1)(S299):
Conference Papers
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PBC: A software framework facilitating pattern-based clustering for microarray data analysis
Proceedings - 2009 International Joint Conference on Bioinformatics, Systems Biology and Intelligent Computing, IJCBS 2009 2009 Jan;30-36
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Time lapse imaging techniques for comparison of mineralization dynamics in primary murine osteoblasts and the late osteoblast/early osteocyte-like cell line MLO-A5.
Cells, tissues, organs 2009 Jan;189(1-4):6-11
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Meta analysis of microarray data using gene regulation pathways
Proceedings - IEEE International Conference on Bioinformatics and Biomedicine, BIBM 2008 2008 Jan;37-42
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Reverse engineering of gene regulatory network by integration of prior global gene regulatory information
Proceedings - IEEE International Conference on Bioinformatics and Biomedicine, BIBM 2008 2008 Jan;129-134
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A computational inference framework for analyzing gene regulation pathway using microarray data
Proceedings - Sixth IEEE Symposium on BioInformatics and BioEngineering, BIBE 2006 2006 Jan;299-306
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Reduction of gene expression by a modified U1 snRNA
Matrix Biology 1998 Jan;17(2):163-164
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Current knowledge and research directions in heritable disorders of connective tissue.
Matrix biology : journal of the International Society for Matrix Biology 1996 Sep;15(4):211-29
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Errors of type I collagen mRNA splicing in osteogenesis imperfecta
Calcium regulation and bone metabolism. Basic and clinical aspects: proceedings of the 10th International Conference on calcium regulating hormones and bone metabolism. ICS886 1990 Jan;207-213
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Transcriptional regulation of type I collagen synthesis by 1,25-dihydroxyvitamin D<sub>3</sub> in bone cells
Calcium regulation and bone metabolism. Basic and clinical aspects: proceedings of the 10th International Conference on calcium regulating hormones and bone metabolism. ICS886 1990 Jan;163-167
Other
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Regulation of collagen synthesis in fetal rat calvaria by 1,25-dihydroxyvitamin D<sub>3</sub>
Calcified Tissue International 1981 Jan;33(3):337
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Symposium on sensorineural hearing loss in children: early detection and intervention. Hearing aids for young children.
Otolaryngologic clinics of North America 1975 Feb;8(1):125-41
Reviews
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Genetics of aging bone.
Mammalian genome : official journal of the International Mammalian Genome Society 2016 Jun;27367-80
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Skeletal development, bone remodeling, and hematopoiesis.
Immunological reviews 2005 Dec;2087-18
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Hormonal regulation of collagen gene expression in osteoblastic cell-overview and new findings.
Connective tissue research 1989 Jan;20(1-4):187-92