Justin D. Radolf, MDProfessor of Medicine, Pediatrics, Molecular Biology and Biophysics, Genetics and Genome Sciences, and ImmunologyDirector of Research, Department of Medicine Senior Scientific Advisor, Connecticut Children’s Medical Center
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Degree | Institution | Major |
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BS | Yale University | Biology |
MD | University of California, San Francisco | Medicine |
Post-Graduate Training
Training | Institution | Specialty |
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Summer Training | Jackson Laboratories | Summer Science Training Program |
Summer Training | Brookhaven National Laboratories | Summer Science Training Program |
Residency | University of Pennsylvania | Internal Medicine |
Fellowship | UCLA | Infectious Diseases |
Fellowship | UCLA | Postdoctoral Research Fellow |
Awards
Name of Award/Honor | Awarding Organization |
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Invited speaker, 36th Annual Alumni Class of 1947 STD Lectureship, Indiana University School of Medicine | Indiana University School of Medicine |
Fellowship in the American Academy of Microbiology | American Society for Microbiology |
Chair, Gordon Research Conference on the Biology of Spirochetes | Gordon Research |
Vice-Chair Gordon Research Conference on the Biology of Spirochetes | Gordon Research |
Merit Award | NIH/NIAID |
Established Investigatorship Award, 1992-97 | American Heart Association |
Scholar, 1989-92 | American Foundation for AIDS Research |
Pfizer Scholars Award for New Faculty 1986-88 | Pfizer |
Young Investigator Award | American Society for Microbiology |
Pfizer Medical Research Merit Award | PFIZER CORPORATION |
Postdoctoral Research Fellowship 1984-86 | American Social Health Association |
National Merit Scholar |
Name & Description | Category | Role | Type | Scope | Start Year | End Year |
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NIH/NIAID Special Emphasis Panel "NIH Support for Conferences and Scientific Meetings (Parent R13 Clinical Trial not Allowed)" | Study Section | Ad hoc reviewer | External | National | 2023 | 2023 |
Clinical Research and Innovation Seed Program (CRISP) | Study Section | Co-Chair | UConn-Storrs | Regional | 2022 | 2023 |
Frontiers Journals | Other | Co-editor | External | International | 2022 | 2022 |
Molecular Microbiology | Editorial Board | Reviewer | External | International | 2018 | |
UConn Microbiome Research Seed Grants Review Panel | Research Committee | Reviewer | UConn-Storrs | University | 2018 | |
Dean’s Special Task Force on Predatory Journals and Conferences | Advisory Committee | Lecturer | UConn Health | University | 2018 | 2019 |
NIH/NIAID Bacterial Pathogenesis Study Section | Advisory Committee | External | National | 2017 | ||
NIH Workshop on the Development of a Syphilis Vaccine | Advisory Committee | External | National | 2017 | ||
UConn Microbiome Research Seed Grants Review Panel | Advisory Committee | UConn Health | State | 2017 | ||
American Academy of Microbiology | Professional/Scientific Organization | Member | External | International | 2017 | |
Advisory Board, Bay Area Lyme Foundation | Advisory Committee | External | National | 2016 | ||
UConn Health Graduate School Admissions Committee | Education Committee | Member, representing Immunology Graduate Program | UConn Health | University | 2016 | 2019 |
NIH/NIAID Study Section: Topics in Bacterial Pathogenesis (IDM-B 80), March 2-3, 2015 | Advisory Committee | External | National | 2015 | ||
NIH/NIAD Study Section: Topics in Bacterial Pathogenesis (IMDM-B 80), October 29-30, 2015 | Advisory Committee | External | National | 2015 | ||
Infection and Immunity | Editorial Board | External | National | 2015 | 2017 | |
Immunology of B Cells in Syphilis (IBIS): a project to use state of the art methodologies for the study of syphilis involving the University of Connecticut Health Center, the University of North Carolina at Chapel Hill, the Duke University Vaccine, and the Guangzhou, China Sexually Transmitted Diseases Control Unit. | Advisory Committee | External | National | 2014 | ||
F13 Infectious Diseases and Microbiology Fellowship Panel: ZRG1 F13-C (20) L, March 20-21, 2014 | Advisory Committee | External | National | 2014 | ||
NIH/NIAID Study Section: Topics in Bacterial Pathogenesis (IDM-B 80), October 26-27, 2014 | Advisory Committee | External | National | 2014 | ||
Planning committee and Scientific Faculty for the 2013 International Conference on Lyme Boreliosis and other Tick Borne Diseases, Boston, MA | Advisory Committee | Reviewer | External | National | 2013 | |
Reviewer for the 19th International Society for STD Research (ISSTDR), London, UK | Advisory Committee | Reviewer | External | International | 2009 | |
NASA: Special emphasis panel reviewing proposals for RFA entitled “Research Opportunities for Fundamental Space Biology Investigations in Microbial, Plant and Cell Biology”. | Advisory Committee | Reviewer | External | National | 2009 | |
CDC Task Force to produce recommendations for the Laboratory Diagnosis of Chlamydia trachomatis, Neisseria gonorrhoeae, and Treponema pallidum | Advisory Committee | Member | External | National | 2009 | |
Reviewer for the 19th International Society for STD Research (ISSTDR) biannual meeting, London, UK | Advisory Committee | Reviewer | External | International | 2009 | |
NIH/NIAID: Special emphasis panel reviewing applications for the Regional Centers of Excellence for Biodefense and Emerging Infectious Diseases | Advisory Committee | Panel member | External | National | 2008 | |
Molecular Microbiology | Editorial Board | External | National | 2008 | 2011 | |
Reviewer for the 17th International Society for STD Research (ISSTDR) biannual meeting, Seattle, WA | Advisory Committee | Reviewer | External | International | 2007 | |
Journal of Infectious Diseases | Editorial Board | Reviewer | External | International | 2007 | |
Journal of Infectious Diseases | Editorial Board | Member of Editorial Advisory Board | External | National | 2007 | |
Ad hoc reviewer for the Medical Research Council, United Kingdom | Advisory Committee | Ad Hoc Reviewer | External | International | 2005 | |
Reviewer for the 15th International Society for STD Research (ISSTDR) biannaual meeting, Amsterdam, The Netherlands | Advisory Committee | Reviewer | External | International | 2005 | |
NIAID special emphasis panels reviewing grants or contracts on chancroid molecular/cellular pathogenesis | Advisory Committee | Reviewer | External | National | 2002 | |
Scientific Advisory Board for the Ninth International Conference on Lyme Borreliosis | Advisory Committee | Member | External | International | 2002 | |
NHLBI Special Emphasis Panel reviewing grant applications in response to RFA entitled “The Role of Infectious Agents in Vascular Disease” | Advisory Committee | Panel member | External | National | 2002 | |
Infectious Diseases and Microbiology Integrated Research Group Study Section Boundaries Team, Center for Scientific Review, National Institutes of Health | Study Section | Member | External | National | 2002 | |
NIGMS special emphasis panel reviewing large scale collaborative research (GLUE) grant applications | Advisory Committee | Member | External | National | 2001 | |
NIGMS/NIAID Special Emphasis Panel reviewing grant applications in response to RFA entitled “Infectious Etiology of Chronic Diseases: novel approaches to pathogen detection” | Advisory Committee | Panel member | External | National | 2001 | |
NIAID special emphasis panels reviewing grants or contracts on Vaccine research centers | Advisory Committee | Reviewer | External | National | 2000 | |
NIAID special emphasis panels reviewing grants or contracts on B. burgdorferi molecular genetics | Advisory Committee | Reviewer | External | National | 2000 | |
NIAID special emphasis panels reviewing grants or contracts on neuroborreliosis | Advisory Committee | Reviewer | External | National | 1998 | |
American Society for Clinical Investigation | Professional/Scientific Organization | Member | External | National | 1997 | |
NIAID special emphasis panels reviewing grants or contracts on chronic Lyme disease | Advisory Committee | Reviewer | External | National | 1997 | |
NIAID special emphasis panels reviewing grants or contracts on tick-mammalian host interactions in Lyme disease | Advisory Committee | Reviewer | External | National | 1996 | |
NIH Advisory Panel on Studies of Chronic Lyme disease | Advisory Committee | Panel member | External | National | 1996 | 2001 |
Current Immunology Reviews Infection and Immunity | Editorial Board | Editorial Board | External | National | 1995 | 2003 |
Scientific advisory council for the 11th bi-annual meeting of the International Society for STD Research | Advisory Committee | Member | External | International | 1995 | |
Bacteriology and Mycology 1 Study Section | Study Section | Member | External | National | 1995 | 1999 |
Infection and Immunity | Editorial Board | External | National | 1995 | 2003 | |
Sexually Transmitted Diseases | Editorial Board | External | Regional | 1995 | 2014 | |
NIAID special emphasis panels reviewing grants or contracts on Lyme disease pathogenesis | Advisory Committee | Reviewer | External | National | 1991 | |
NIAID special emphasis panels reviewing grants or contracts on mycoplasma and HIV infection | Advisory Committee | Reviewer | External | National | 1991 | |
NIAID special emphasis panels reviewing grants or contracts on animal models of Lyme disease | Advisory Committee | Reviewer | External | National | 1991 | |
National Arthritis Foundation Clinical and Applied Immunology study section | Study Section | Study Section Reviewer | External | National | 1991 | 1993 |
Lyme Disease/Borrelia burgdorferi
The Lyme disease spirochete B. urgdorferi is maintained in nature via an enzootic cycle in which it is transmitted by the nymphal stage of its vector, the deer tick Ixodes scapularis, to a mammalian host (typically the white-footed mouse Peromyscus leucopus) and then is acquired when a naïve larval tick feeds on an infected mouse. Infection of humans with B. burgdorferi is accidental and is not required for persistence of the spirochete in nature. In order to transit between its arthropod and mammalian hosts, spirochetes must decipher complex environmental cues delivered at the feeding site and, in response, undergo dramatic changes in their transcriptomes and proteomes. The principal objective of Lyme disease research conducted in the Radolf Laboratory is to understand these processes. The alternative sigma factor RpoS is a unifying genetic feature of this project. Signals delivered by the blood meal induce the expression of RpoS which, as the promoter-reading subunit of RNA polymerase, induces far-reaching changes in the bacterium’s transcriptome: (1) upregulation of approximately 100 B. burgdorferi genes that we believe are required for transmission of spirochetes from tick to mouse and/or the establishment of infection once within the mouse and (2) downregulation of approximately 30 tick-phase genes that spirochetes no longer need once they are inoculated into mice.
Critical to this work has been our development of green fluorescent protein (GFP) reporters that enable us to track live spirochetes in ticks and mice. Our live-imaging studies have fundamentally changed our understanding of the transmission process. In order to reach the mouse, spirochetes disseminate through the midgut into the salivary glands in order to access the salivary stream which they “ride” into the vertebrate host. We have found that dissemination of spirochetes in ticks is actually biphasic. In the first phase, which we have termed “adherence-mediated migration, spirochetes replicate in close association with differentiating midgut epithelial cells, “working” their way as aggregates or networks to the base of the epithelium. In the second phase, they transition into typically motile spirochetes, complete the penetration through the midgut, and then move on to the salivary glands en route to the mouse. Most recently, we have found that spirochetes lacking RpoS are deficient in this process and we are developing various strategies to identify the RpoS-dependent genes involved.
Syphilis/Treponema pallidum
The syphilis spirochete T. pallidum harbors many resemblances to B. burgdorferi but actually employs a markedly different parasitic strategy. Whereas B. burgdorferi is an enzootic pathogen, T. pallidum is an obligate pathogen of humans which cannot be cultivated in artificial medium. The modes of transmission of the two bacteria differ markedly as well: T. pallidum is transmitted from person-to-person by sexual activity, whereas B. burgdorferi is transmitted by an arthropod vector. Once within the host, T. pallidum begins to replicate locally, eventually causing a genital ulcer, called a chancre, the clinical hallmark of the primary stage of the disease. As the chancre develops, treponemes begin to make their way towards draining lymph nodes and blood vessels in order to spread systemically. Once within the blood, T. pallidum is extremely adept at invading virtually every organ system in the body, including the central nervous system, and establishing persistent infection that can cause serious, even life threatening, complications months to years later. We have designated T. pallidum “the stealth pathogen” because of its remarkable ability to evade host immune defenses.
Efforts in the Radolf Laboratory to explain T. pallidum’s stealth pathogenicity have focused on the bacterium’s unusual molecular architecture. Over the years, we have generated abundant evidence that the T. pallidum outer membrane differs markedly in structure and composition from its gram-negative counterparts (e.g., Escherichia coi). Not only does it lack lipopolysaccharide, the highly inflammatory glycolipid in the outer membranes of gram negative bacteria, it also contains a much lower density of integral membrane proteins that present few surface antigenic targets to the host immune system. Situated below the outer bilayer, where they are inaccessible to antibodies in intact organisms, are the bacterium’s major immunogens, many of which are periplasmic proteins tethered by N-terminal lipids to the cytoplasmic membrane. This work ushered in what we have termed “the quest” for T. pallidum outer membrane proteins, a project that has been ongoing for more than 20 years. Why a quest? Because identifying rare outer membrane proteins is so difficult and requires extraordinary commitment. Fortunately, we now have much more powerful tools to fulfill the quest, among which is the complete genomic sequence of T. pallidum. Genome mining, however, isn’t as easy as it sounds because, with one exception, there are no proteins in the T. pallidum genome with sequence relatedness to well characterized outer membrane proteins of gram-negatives. This work is complicated further by the fragility of the treponeme’s outer membrane. Our genereal strategy is to use bioinformatics algorithms to identify outer membrane protein candidates that then must be cloned, expressed, purified, structurally characterized, and localized in live treponemes. Why is this quest important? Two reasons. First, outer membrane proteins provide channels through which bacteria obtain nutrients. Second, we believe that these surface-exposed proteins, few as they are, are likely vaccine candidates. Given the explosive increase in syphilis cases in the United States and the world during the past decade, a vaccine would be a major weapon in our battle against this centuries old affliction of humans.
In order to fulfill its genetic destiny as a stealth pathogen, T. pallidum must acquire nutrients in every milieu within its obligate human host in which it finds itself, while fending off the host’s attempts to undermine its homeostasis. Recognition of this metabolic war between pathogen and host led us to explore other facets of T. pallidum virulence. One has been transition metal acquisition. Transition metals, such as iron, manganese, and zinc, are essential for life but are present in mammalian body fluids at exceedingly low concentrations. Bacterial pathogens, T. pallidum being no exception, employ highly developed strategems to obtain these nutrients. Our work along these lines has centered about characterizing two ABC transporters within the cytoplasmic membrane (Tro and Znu) that work cooperatively to meet the bacterium’s metal requirements. Lastly, our immune system uses toxic compounds called reactive oxygen species, to kill bacteria. T. pallidum has extremely robust enzymatic mechanisms for detoxifying reactive oxygen species. Understanding how these enzymes work and are regulated in response to host defenses is relevant to all bacterial diseases, not just syphilis.
Accepting Lab Rotation Students: Fall Block 2024, Spring 1 and 2 Block 2025
Lab Rotation Projects
Molecular Pathogenesis of Syphilis and Lyme Disease
1. Differential gene expression by Borrelia burgdorferi, the Lyme disease spirochete. B. burgdorferi undergoes dramatic changes in gene expression and protein composition as it cycles back and forth between its arthropod vector (deer ticks) and mammalian host (mice and other rodents). We have developed a variety of genetic techniques that, in conjunction with microarray analysis, are enabling us to decipher the patterns of borrelial gene expression at various points in the enzootic cycle, the mechanisms that regulate differential gene expression, and the relevant signal transduction mechanisms.
2. Outer membrane architecture of Treponema pallidum, the syphilis spirochete. We demonstrated a number of years ago that the T. pallidum outer membrane differs markedly in ultrastructure and composition from the “conventional” outer membranes of gram-negative bacteria, such as E. coli. We have identified a novel T. pallidum outer membrane protein that integrates into the outer membrane lipid bilayer via covalently bound lipids and amphipathic helices. This protein also appears to promote solute uptake by destabilizing the lipid bilayer, creating pores. Using model membrane systems, we would like to understand more about the structure-function relationships of this unusual molecule.
Journal Articles
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Development and utilization of Treponema pallidum expressing green fluorescent protein to study spirochete-host interactions and antibody-mediated clearance: expanding the toolbox for syphilis research.
mBio 2024 Nov;e0325324
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An atlas of human vector-borne microbe interactions reveals pathogenicity mechanisms.
Cell 2024 Jul;187(15):4113-4127.e13
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New Pathways in Syphilis Vaccine Development.
Sexually transmitted diseases 2024 Jul;
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Clinical presentation of early syphilis and genomic sequences of Treponema pallidum strains in patient specimens and isolates obtained by rabbit inoculation.
The Journal of infectious diseases 2024 Jun;
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Treponema pallidum genetic diversity and its implications for targeted vaccine development: A cross-sectional study of early syphilis cases in Southwestern Colombia.
PloS one 2024 Jan;19(7):e0307600
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BosR and PlzA reciprocally regulate RpoS function to sustain Borrelia burgdorferi in ticks and mammals.
The Journal of clinical investigation 2023 Jan;
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Use of Epivolve phage display to generate a monoclonal antibody with opsonic activity directed against a subdominant epitope on extracellular loop 4 of Treponema pallidum BamA (TP0326).
Frontiers in immunology 2023 Jan;141222267
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Extracellular Loops of the Treponema pallidum FadL Orthologs TP0856 and TP0858 Elicit IgG Antibodies and IgG+-Specific B-Cells in the Rabbit Model of Experimental Syphilis.
mBio 2022 Aug;13(4):e0163922
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A suite of PCR-LwCas13a assays for detection and genotyping of Treponema pallidum in clinical samples.
Nature communications 2022 Aug;13(1):4671
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Bacterial Indole as a Multifunctional Regulator of Klebsiella oxytoca Complex Enterotoxicity.
mBio 2022 Jan;e0375221
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High-throughput nanopore sequencing of Treponema pallidum tandem repeat genes arp and tp0470 reveals clade-specific patterns and recapitulates global whole genome phylogeny.
Frontiers in microbiology 2022 Jan;131007056
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PlzA is a bifunctional c-di-GMP biosensor that promotes tick and mammalian host-adaptation of Borrelia burgdorferi.
PLoS pathogens 2021 Jul;17(7):e1009725
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Macrophage mediated recognition and clearance of Borrelia burgdorferi elicits MyD88-dependent and -independent phagosomal signals that contribute to phagocytosis and inflammation.
BMC immunology 2021 May;22(1):32
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Structural modeling of the Treponema pallidum OMPeome: a roadmap for deconvolution of syphilis pathogenesis and development of a syphilis vaccine.
Journal of bacteriology 2021 May;
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Host-specific functional compartmentalization within the oligopeptide transporter during the Borrelia burgdorferi enzootic cycle.
PLoS pathogens 2021 Jan;17(1):e1009180
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Lyme Disease in Humans.
Current issues in molecular biology 2021 Jan;42333-384
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Outcomes from re-treatment and cerebrospinal fluid analyses in syphilis patients who had serological non-response or lack of seroreversion after initial therapy.
Sexually transmitted diseases (in press) 2020 Oct;
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Evidence that immunization with TP0751, a bipartite Treponema pallidum lipoprotein with an intrinsically disordered region and lipocalin fold, fails to protect in the rabbit model of experimental syphilis.*Shared First Authorship
PLoS pathogens 2020 Sep;16(9):e1008871
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Analysis of Treponema pallidum strains from China using improved methods for whole-genome sequencing from primary syphilis chancres.
The Journal of Infectious Diseases (in press) 2020 Jul;
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The RpoS Gatekeeper in Borrelia burgdorferi: An Invariant Regulatory Scheme That Promotes Spirochete Persistence in Reservoir Hosts and Niche Diversity.
Frontiers in microbiology 2019 Sep;101923
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Sequence Variation of Rare Outer Membrane Protein β-Barrel Domains in Clinical Strains Provides Insights into the Evolution of Treponema pallidum subsp. pallidum, the Syphilis Spirochete.
mBio 2018 Jun;9(3):
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Peptide Uptake Is Essential for Borrelia burgdorferi Viability and Involves Structural and Regulatory Complexity of its Oligopeptide Transporter.
mBio 2017 Dec;8(6):
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The major outer sheath protein forms distinct conformers and multimeric complexes in the outer membrane and periplasm of Treponema denticola.
Scientific reports 2017 Oct;7(1):13260
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Two Distinct Mechanisms Govern RpoS-Mediated Repression of Tick-Phase Genes during Mammalian Host Adaptation by Borrelia burgdorferi, the Lyme Disease Spirochete.
mBio 2017 Aug;8(4):
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IFNγ Enhances CD64-Potentiated Phagocytosis of Treponema pallidum Opsonized with Human Syphilitic Serum by Human Macrophages.
Frontiers in immunology 2017 Jan;81227
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Consensus computational network analysis for identifying candidate outer membrane proteins from Borrelia spirochetes.
BMC microbiology 2016 Jan;16(1):141
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Genomic insights into the Ixodes scapularis tick vector of Lyme disease.
Nature Communications 2016 Jan;710507
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Structural characterization and modeling of the Borrelia burgdorferi hybrid histidine kinase Hk1 periplasmic sensor: A system for sensing small molecules associated with tick feeding.
Journal of structural biology 2015 Aug;192(1):48-58
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Cyclic di-GMP modulates gene expression in Lyme disease spirochetes at the tick-mammal interface to promote spirochete survival during the blood meal and tick-to-mammal transmission.
Infection and immunity 2015 May;83(8):3043-60
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A Homology Model Reveals Novel Structural Features and an Immunodominant Surface Loop/Opsonic Target in the Treponema pallidum BamA Ortholog TP_0326.
Journal of bacteriology 2015 Mar;197(11):1906-20
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Bipartite Topology of Treponema pallidum Repeat Proteins C/D and I: OUTER MEMBRANE INSERTION, TRIMERIZATION, AND PORIN FUNCTION REQUIRE A C-TERMINAL β-BARREL DOMAIN.
The Journal of biological chemistry 2015 Mar;290(19):12313-31
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A systematic review of syphilis serological treatment outcomes in HIV-infected and HIV-uninfected persons: rethinking the significance of serological non-responsiveness and the serofast state after therapy.
BMC infectious diseases 2015 Jan;15479
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Correction for Anand et al., The Major Outer Sheath Protein (Msp) of Treponema denticola Has a Bipartite Domain Architecture and Exists as Periplasmic and Outer Membrane-Spanning Conformers.
Journal of bacteriology 2014 Sep;196(18):3361
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Correction for Anand et al., TprC/D (Tp0117/131), a Trimeric, Pore-Forming Rare Outer Membrane Protein of Treponema pallidum, Has a Bipartite Domain Structure.
Journal of bacteriology 2014 Sep;196(18):3360
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HrpA, an RNA helicase involved in RNA processing, is required for mouse infectivity and tick transmission of the Lyme disease spirochete.
PLoS pathogens 2013 Dec;9(12):e1003841
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Characterization of outer membranes isolated from Treponema pallidum, the syphilis spirochete.
Infection and immunity 2013 Nov;63(11):4244-52
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TP0453, a concealed outer membrane protein of Treponema pallidum, enhances membrane permeability.
Journal of bacteriology 2013 Sep;187(18):6499-508
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The major outer sheath protein (Msp) of Treponema denticola has a bipartite domain architecture and exists as periplasmic and outer membrane-spanning conformers.
Journal of bacteriology 2013 May;195(9):2060-71
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Pulling the trigger on lyme arthritis.
The Journal of infectious diseases 2013 Mar;207(6):877-9
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TprC/D (Tp0117/131), a trimeric, pore-forming rare outer membrane protein of Treponema pallidum, has a bipartite domain structure.
Journal of bacteriology 2012 May;194(9):2321-33
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The heterogeneous motility of the Lyme disease spirochete in gelatin mimics dissemination through tissue.
Proceedings of the National Academy of Sciences of the United States of America 2012 Feb;109(8):3059-64
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Borrelia burgdorferi requires the alternative sigma factor RpoS for dissemination within the vector during tick-to-mammal transmission.
PLoS pathogens 2012 Feb;8(2):e1002532
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CD14 cooperates with complement receptor 3 to mediate MyD88-independent phagocytosis of Borrelia burgdorferi.
Proceedings of the National Academy of Sciences of the United States of America 2012 Jan;109(4):1228-32
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The transition from closed to open conformation of Treponema pallidum outer membrane-associated lipoprotein TP0453 involves membrane sensing and integration by two amphipathic helices.
The Journal of biological chemistry 2011 Dec;286(48):41656-68
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The coenzyme A disulphide reductase of Borrelia burgdorferi is important for rapid growth throughout the enzootic cycle and essential for infection of the mammalian host.
Molecular microbiology 2011 Nov;82(3):679-97
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Borrelia burgdorferi requires glycerol for maximum fitness during the tick phase of the enzootic cycle.
PLoS pathogens 2011 Jul;7(7):e1002102
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TP0326, a Treponema pallidum β-barrel assembly machinery A (BamA) orthologue and rare outer membrane protein.
Molecular microbiology 2011 Jun;80(6):1496-515
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BB0844, an RpoS-regulated protein, is dispensable for Borrelia burgdorferi infectivity and maintenance in the mouse-tick infectious cycle.
Infection and immunity 2011 Mar;79(3):1208-17
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Phagosomal signaling by Borrelia burgdorferi in human monocytes involves Toll-like receptor (TLR) 2 and TLR8 cooperativity and TLR8-mediated induction of IFN-beta.
Proceedings of the National Academy of Sciences of the United States of America 2011 Mar;108(9):3683-8
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Syphilis screening using a reverse sequence with treponemal tests—United States, 2006-2010.
Morbidity & Mortality Weekly Report 2011 Jan;60133-137
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Surface immunolabeling and consensus computational framework to identify candidate rare outer membrane proteins of Treponema pallidum.
Infection and immunity 2010 Dec;78(12):5178-94
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Znu is the predominant zinc importer in Yersinia pestis during in vitro growth but is not essential for virulence.
Infection and immunity 2010 Dec;78(12):5163-77
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Broad specificity AhpC-like peroxiredoxin and its thioredoxin reductant in the sparse antioxidant defense system of Treponema pallidum.
Proceedings of the National Academy of Sciences of the United States of America 2010 Apr;107(14):6240-5
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Identification of residues within ligand-binding domain 1 (LBD1) of the Borrelia burgdorferi OspC protein required for function in the mammalian environment.
Molecular microbiology 2010 Apr;76(2):393-408
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A TFIIH-associated mediator head is a basal factor of small nuclear spliced leader RNA gene transcription in early-diverged trypanosomes.
Molecular and cellular biology 2010 Jan;30(23):5502-13
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Role of acetyl-phosphate in activation of the Rrp2-RpoN-RpoS pathway in Borrelia burgdorferi.
PLoS pathogens 2010 Jan;6(9):e1001104
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Secondary syphilis in cali, Colombia: new concepts in disease pathogenesis.
PLoS neglected tropical diseases 2010 Jan;4(5):e690
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Cryo-electron tomography elucidates the molecular architecture of Treponema pallidum, the syphilis spirochete.
Journal of bacteriology 2009 Dec;191(24):7566-80
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Live imaging reveals a biphasic mode of dissemination of Borrelia burgdorferi within ticks.
The Journal of clinical investigation 2009 Dec;119(12):3652-65
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Activation of human monocytes by live Borrelia burgdorferi generates TLR2-dependent and -independent responses which include induction of IFN-beta.
PLoS pathogens 2009 May;5(5):e1000444
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Borrelia burgdorferi bba74 is expressed exclusively during tick feeding and is regulated by both arthropod- and mammalian host-specific signals.
Journal of bacteriology 2009 Apr;191(8):2783-94
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Local production of IFN-gamma by invariant NKT cells modulates acute Lyme carditis.
Journal of immunology (Baltimore, Md. : 1950) 2009 Mar;182(6):3728-34
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NKT cells prevent chronic joint inflammation after infection with Borrelia burgdorferi.
Proceedings of the National Academy of Sciences of the United States of America 2008 Dec;105(50):19863-8
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MicroCommentary: The long strange trip of Borrelia burgdorferi OspC.
Mol Micro 2008 Jan;69(1):1-4
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Phagocytosis of Borrelia burgdorferi, the Lyme disease spirochete, potentiates innate immune activation and induces apoptosis in human monocytes.
Infection and immunity 2008 Jan;76(1):56-70
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Analysis of the RpoS regulon in Borrelia burgdorferi in response to mammalian host signals provides insight into RpoS function during the enzootic cycle.
Molecular microbiology 2007 Sep;65(5):1193-217
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The general transition metal (Tro) and Zn2+ (Znu) transporters in Treponema pallidum: analysis of metal specificities and expression profiles.
Molecular microbiology 2007 Jul;65(1):137-52
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Assessment of the kinetics of Treponema pallidum dissemination into blood and tissues in experimental syphilis by real-time quantitative PCR.
Infection and immunity 2007 Jun;75(6):2954-8
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Phagocytosis of Borrelia burgdorferi and Treponema pallidum potentiates innate immune activation and induces gamma interferon production.
Infection and immunity 2007 Apr;75(4):2046-62
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Treponema pallidum elicits innate and adaptive cellular immune responses in skin and blood during secondary syphilis: a flow-cytometric analysis.
The Journal of infectious diseases 2007 Mar;195(6):879-87
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Borrelia burgdorferi BBA74, a periplasmic protein associated with the outer membrane, lacks porin-like properties.
Journal of bacteriology 2007 Mar;189(5):2063-8
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The Lyme disease agent Borrelia burgdorferi requires BB0690, a Dps homologue, to persist within ticks.
Molecular microbiology 2007 Feb;63(3):694-710
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Sigma factor selectivity in Borrelia burgdorferi: RpoS recognition of the ospE/ospF/elp promoters is dependent on the sequence of the -10 region.
Molecular microbiology 2006 Mar;59(6):1859-75
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Alternate sigma factor RpoS is required for the in vivo-specific repression of Borrelia burgdorferi plasmid lp54-borne ospA and lp6.6 genes.
Journal of bacteriology 2005 Nov;187(22):7845-52
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Lipoprotein-dependent and -independent immune responses to spirochetal infection.
Clinical and diagnostic laboratory immunology 2005 Aug;12(8):949-58
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Signaling through CD14 attenuates the inflammatory response to Borrelia burgdorferi, the agent of Lyme disease.
Journal of immunology (Baltimore, Md. : 1950) 2005 Feb;174(3):1539-48
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Analysis of promoter elements involved in the transcriptional initiation of RpoS-dependent Borrelia burgdorferi genes.
Journal of bacteriology 2004 Nov;186(21):7390-402
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RpoS is not central to the general stress response in Borrelia burgdorferi but does control expression of one or more essential virulence determinants.
Infection and immunity 2004 Nov;72(11):6433-45
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Experimental assessment of the roles of linear plasmids lp25 and lp28-1 of Borrelia burgdorferi throughout the infectious cycle.
Infection and immunity 2004 Oct;72(10):5938-46
-
Borrelia burgdorferi outer surface protein (osp) B expression independent of ospA.
Microbial pathogenesis 2004 Jul;37(1):35-40
-
Borrelia burgdorferi transcriptome in the central nervous system of non-human primates.
Proceedings of the National Academy of Sciences of the United States of America 2003 Dec;100(26):15953-8
-
Coevolution of markers of innate and adaptive immunity in skin and peripheral blood of patients with erythema migrans.
Journal of immunology (Baltimore, Md. : 1950) 2003 Sep;171(5):2660-70
-
Molecular characterization of the presence of Eubacterium spp and Streptococcus spp in endodontic infections.
Oral microbiology and immunology 2003 Aug;18(4):249-55
-
The Treponema pallidum tro operon encodes a multiple metal transporter, a zinc-dependent transcriptional repressor, and a semi-autonomously expressed phosphoglycerate mutase.
The Journal of biological chemistry 2003 Jun;278(23):20687-94
-
A plasmid-encoded nicotinamidase (PncA) is essential for infectivity of Borrelia burgdorferi in a mammalian host.
Molecular microbiology 2003 May;48(3):753-64
-
Increasing health burden of human babesiosis in endemic sites.
The American journal of tropical medicine and hygiene 2003 Apr;68(4):431-6
-
Profiling of temperature-induced changes in Borrelia burgdorferi gene expression by using whole genome arrays.
Infection and immunity 2003 Apr;71(4):1689-705
-
Adaptation of the Lyme disease spirochaete to the mammalian host environment results in enhanced glycosaminoglycan and host cell binding.
Molecular microbiology 2003 Mar;47(5):1433-44
-
Characterization of the stringent response and rel(Bbu) expression in Borrelia burgdorferi.
Journal of bacteriology 2003 Feb;185(3):957-65
-
Environmental regulation and differential production of members of the Bdr protein family of Borrelia burgdorferi.
Infection and immunity 2002 Dec;70(12):7033-41
-
Cellular immunity, but not gamma interferon, is essential for resolution of Babesia microti infection in BALB/c mice.
Infection and immunity 2002 Sep;70(9):5304-6
-
PCR-based identification of bacteria associated with endodontic infections.
Journal of clinical microbiology 2002 Sep;40(9):3223-31
-
Disease-specific diagnosis of coinfecting tickborne zoonoses: babesiosis, human granulocytic ehrlichiosis, and Lyme disease.
Clinical infectious diseases : an official publication of the Infectious Diseases Society of America 2002 May;34(9):1184-91
-
Clonal polymorphism of Borrelia burgdorferi strain B31 MI: implications for mutagenesis in an infectious strain background.
Infection and immunity 2002 Apr;70(4):2139-50
-
The crystal structure of Zn(II)-free Treponema pallidum TroA, a periplasmic metal-binding protein, reveals a closed conformation.
Journal of bacteriology 2002 Apr;184(8):2300-4
-
Clinical manifestations of early syphilis by HIV status microbial inoculation in a type I diabetic mouse model.
J Endodon 2002 Jan;288
-
Contribution of neelaredoxin to oxygen tolerance by Treponema pallidum.
Methods in enzymology 2002 Jan;353140-56
-
Identification of loci critical for replication and compatibility of a Borrelia burgdorferi cp32 plasmid and use of a cp32-based shuttle vector for the expression of fluorescent reporters in the lyme disease spirochaete.
Molecular microbiology 2002 Jan;43(2):281-95
-
Periapical lesion progression with controlled microbial inoculation in a type I diabetic mouse model.
Journal of endodontics 2002 Jan;28(1):8-16
-
Diagnosis of babesiosis using an immunoblot serologic test.
Clinical and diagnostic laboratory immunology 2001 Nov;8(6):1177-80
-
Release of Toll-like receptor-2-activating bacterial lipoproteins in Shigella flexneri culture supernatants.
Infection and immunity 2001 Oct;69(10):6248-55
-
Toll-like receptor 2-dependent inhibition of macrophage class II MHC expression and antigen processing by 19-kDa lipoprotein of Mycobacterium tuberculosis.
Journal of immunology (Baltimore, Md. : 1950) 2001 Jul;167(2):910-8
-
Discrimination of bacterial lipoproteins by Toll-like receptor 6.
International immunology 2001 Jul;13(7):933-40
-
Regulation of OspE-related, OspF-related, and Elp lipoproteins of Borrelia burgdorferi strain 297 by mammalian host-specific signals.
Infection and immunity 2001 Jun;69(6):3618-27
-
The TprK protein of Treponema pallidum is periplasmic and is not a target of opsonic antibody or protective immunity.
The Journal of experimental medicine 2001 May;193(9):1015-26
-
Insertion of fluorescent fatty acid probes into the outer membranes of the pathogenic spirochaetes Treponema pallidum and Borrelia burgdorferi.
Microbiology (Reading, England) 2001 May;147(Pt 5):1161-9
-
The cutaneous response in humans to Treponema pallidum lipoprotein analogues involves cellular elements of both innate and adaptive immunity.
Journal of immunology (Baltimore, Md. : 1950) 2001 Mar;166(6):4131-40
-
Clinical manifestations of early syphilis by HIV status and gender: results of the syphilis and HIV study.
Sexually transmitted diseases 2001 Mar;28(3):158-65
-
Neelaredoxin, an iron-binding protein from the syphilis spirochete, Treponema pallidum, is a superoxide reductase.
The Journal of biological chemistry 2000 Sep;275(37):28439-48
-
Decorin-binding protein A (DbpA) of Borrelia burgdorferi is not protective when immunized mice are challenged via tick infestation and correlates with the lack of DbpA expression by B. burgdorferi in ticks.
Infection and immunity 2000 Aug;68(8):4759-64
-
Isolation and molecular cloning of a secreted immunosuppressant protein from Dermacentor andersoni salivary gland.
The Journal of parasitology 2000 Jun;86(3):516-25
-
Molecular and evolutionary characterization of the cp32/18 family of supercoiled plasmids in Borrelia burgdorferi 297.
Infection and immunity 2000 Mar;68(3):1574-86
-
Virulent Treponema pallidum, lipoprotein, and synthetic lipopeptides induce CCR5 on human monocytes and enhance their susceptibility to infection by human immunodeficiency virus type 1.
The Journal of infectious diseases 2000 Jan;181(1):283-93
-
Toll-like receptor 2 functions as a pattern recognition receptor for diverse bacterial products.
The Journal of biological chemistry 1999 Nov;274(47):33419-25
-
Identification, characterization, and expression of three new members of the Borrelia burgdorferi Mlp (2.9) lipoprotein gene family.
Infection and immunity 1999 Nov;67(11):6008-18
-
Secondary syphilis in persons infected with and not infected with HIV-1: a comparative immunohistologic study.
The American Journal of dermatopathology 1999 Oct;21(5):432-41
-
Activation of human monocytic cells by Borrelia burgdorferi and Treponema pallidum is facilitated by CD14 and correlates with surface exposure of spirochetal lipoproteins.
Journal of immunology (Baltimore, Md. : 1950) 1999 Aug;163(4):2049-56
-
The Treponema denticola major sheath protein is predominantly periplasmic and has only limited surface exposure.
Infection and immunity 1999 Aug;67(8):4072-83
-
Cell activation and apoptosis by bacterial lipoproteins through toll-like receptor-2.
Science (New York, N.Y.) 1999 Jul;285(5428):736-9
-
Physicochemical evidence that Treponema pallidum TroA is a zinc-containing metalloprotein that lacks porin-like structure.
Journal of bacteriology 1999 Jul;181(14):4420-3
-
Treponema pallidum TroA is a periplasmic zinc-binding protein with a helical backbone.
Nature structural biology 1999 Jul;6(7):628-33
-
Membrane topology and cellular location of the Treponema pallidum glycerophosphodiester phosphodiesterase (GlpQ) ortholog.
Infection and immunity 1999 May;67(5):2266-76
-
Molecular and evolutionary analysis of Borrelia burgdorferi 297 circular plasmid-encoded lipoproteins with OspE- and OspF-like leader peptides.
Infection and immunity 1999 Mar;67(3):1526-32
-
Lyme arthritis synovial gamma delta T cells respond to Borrelia burgdorferi lipoproteins and lipidated hexapeptides.
Journal of immunology (Baltimore, Md. : 1950) 1998 Nov;161(10):5762-71
-
Decorin-binding protein of Borrelia burgdorferi is encoded within a two-gene operon and is protective in the murine model of Lyme borreliosis.
Infection and immunity 1998 Jun;66(6):2674-83
-
Treponema pallidum and Borrelia burgdorferi lipoproteins and synthetic lipopeptides activate monocytic cells via a CD14-dependent pathway distinct from that used by lipopolysaccharide.
Journal of immunology (Baltimore, Md. : 1950) 1998 Jun;160(11):5455-64
-
A new animal model for studying Lyme disease spirochetes in a mammalian host-adapted state.
The Journal of clinical investigation 1998 May;101(10):2240-50
-
Treponema pallidum, lipoproteins, and synthetic lipoprotein analogues induce human immunodeficiency virus type 1 gene expression in monocytes via NF-kappaB activation.
The Journal of infectious diseases 1998 Apr;177(4):941-50
-
Borrelia burgdorferi strain-specific Osp C-mediated immunity in mice.
Infection and immunity 1997 Nov;65(11):4661-7
-
Identification of homologs for thioredoxin, peptidyl prolyl cis-trans isomerase, and glycerophosphodiester phosphodiesterase in outer membrane fractions from Treponema pallidum, the syphilis spirochete.
Infection and immunity 1997 Oct;65(10):4179-89
-
Identification and transcriptional analysis of a Treponema pallidum operon encoding a putative ABC transport system, an iron-activated repressor protein homolog, and a glycolytic pathway enzyme homolog.
Gene 1997 Sep;197(1-2):47-64
-
Tromp1, a putative rare outer membrane protein, is anchored by an uncleaved signal sequence to the Treponema pallidum cytoplasmic membrane.
Journal of bacteriology 1997 Aug;179(16):5076-86
-
A randomized trial of enhanced therapy for early syphilis in patients with and without human immunodeficiency virus infection. The Syphilis and HIV Study Group.
The New England journal of medicine 1997 Jul;337(5):307-14
-
Molecular characterization and cellular localization of TpLRR, a processed leucine-rich repeat protein of Treponema pallidum, the syphilis spirochete.
Journal of bacteriology 1997 May;179(10):3188-95
-
A diffusible cytotoxin of Haemophilus ducreyi.
Proceedings of the National Academy of Sciences of the United States of America 1997 Apr;94(8):4056-61
-
The major outer membrane protein of Haemophilus ducreyi consists of two OmpA homologs.
Journal of bacteriology 1997 Mar;179(5):1764-73
-
Identification of tandem genes involved in lipooligosaccharide expression by Haemophilus ducreyi.
Infection and immunity 1997 Feb;65(2):651-60
-
Molecular characterization of a 6.6-kilodalton Borrelia burgdorferi outer membrane-associated lipoprotein (lp6.6) which appears to be downregulated during mammalian infection.
Infection and immunity 1997 Feb;65(2):412-21
-
Cloning and sequencing of the gene encoding the Cu,Zn-superoxide dismutase of Haemophilus ducreyi.
Gene 1996 Dec;183(1-2):35-40
-
A mgl-like operon in Treponema pallidum, the syphilis spirochete.
Gene 1996 Oct;177(1-2):115-21
-
Activation of human monocytic cells by Treponema pallidum and Borrelia burgdorferi lipoproteins and synthetic lipopeptides proceeds via a pathway distinct from that of lipopolysaccharide but involves the transcriptional activator NF-kappa B.
Infection and immunity 1996 Sep;64(9):3845-52
-
Prominent osseous and unusual dermatologic manifestations of early syphilis in two patients with discordant serological statuses for human immunodeficiency virus infection.
Clinical infectious diseases : an official publication of the Infectious Diseases Society of America 1996 Sep;23(3):462-7
-
Outer surface lipoproteins of Borrelia burgdorferi activate vascular endothelium in vitro.
Infection and immunity 1996 Aug;64(8):3180-7
-
Limited surface exposure of Borrelia burgdorferi outer surface lipoproteins.
Proceedings of the National Academy of Sciences of the United States of America 1996 Jul;93(15):7973-8
-
Borrelia burgdorferi supercoiled plasmids encode multicopy tandem open reading frames and a lipoprotein gene family.
Journal of bacteriology 1996 Jun;178(11):3293-307
-
A hemoglobin-binding outer membrane protein is involved in virulence expression by Haemophilus ducreyi in an animal model.
Infection and immunity 1996 May;64(5):1724-35
-
Evidence for in vivo but not in vitro expression of a Borrelia burgdorferi outer surface protein F (OspF) homologue.
Molecular microbiology 1995 Nov;18(3):507-20
-
Chemiluminescent analysis of Borrelia burgdorferi penicillin-binding proteins using ampicillin conjugated to digoxigenin.
Microbial pathogenesis 1995 Oct;19(4):257-72
-
Detection of Haemophilus ducreyi lipooligosaccharide by means of an immunolimulus assay.
Journal of immunological methods 1995 Sep;185(2):225-35
-
A system for generalized mutagenesis of Haemophilus ducreyi.
Infection and immunity 1995 Aug;63(8):2976-82
-
Membrane topology of Borrelia burgdorferi and Treponema pallidum lipoproteins.
Infection and immunity 1995 Jul;63(7):2424-34
-
Characterization of outer membranes isolated from Borrelia burgdorferi, the Lyme disease spirochete.
Infection and immunity 1995 Jun;63(6):2154-63
-
Treponema pallidum and the quest for outer membrane proteins.
Molecular microbiology 1995 Jun;16(6):1067-73
-
Use of tissue culture and animal models to identify virulence-associated traits of Haemophilus ducreyi.
Infection and immunity 1995 May;63(5):1754-61
-
Dermal inflammation elicited by synthetic analogs of Treponema pallidum and Borrelia burgdorferi lipoproteins.
Infection and immunity 1995 Apr;63(4):1507-15
-
Treponema pallidum and Borrelia burgdorferi lipoproteins and synthetic lipopeptides activate monocytes/macrophages.
Journal of immunology (Baltimore, Md. : 1950) 1995 Mar;154(6):2866-77
-
Treponema pallidum in gel microdroplets: a novel strategy for investigation of treponemal molecular architecture.
Molecular microbiology 1995 Mar;15(6):1151-64
-
The 47-kDa major lipoprotein immunogen of Treponema pallidum is a penicillin-binding protein with carboxypeptidase activity.
Proceedings of the National Academy of Sciences of the United States of America 1994 Nov;91(24):11611-5
-
Virulent Treponema pallidum promotes adhesion of leukocytes to human vascular endothelial cells.
Infection and immunity 1994 Oct;62(10):4622-5
-
Role of outer membrane architecture in immune evasion by Treponema pallidum and Borrelia burgdorferi.
Trends in microbiology 1994 Sep;2(9):307-11
-
Fatty acids of Treponema pallidum and Borrelia burgdorferi lipoproteins.
Journal of bacteriology 1994 Apr;176(8):2151-7
-
Similarity between the 38-kilodalton lipoprotein of Treponema pallidum and the glucose/galactose-binding (MglB) protein of Escherichia coli.
Infection and immunity 1994 Apr;62(4):1381-91
-
Treponema pallidum rare outer membrane proteins: analysis of mobility by freeze-fracture electron microscopy.
Journal of bacteriology 1994 Mar;176(6):1598-608
-
Digoxigenin-ampicillin conjugate for detection of penicillin-binding proteins by chemiluminescence.
Antimicrobial agents and chemotherapy 1994 Feb;38(2):330-6
-
Analysis of Borrelia burgdorferi membrane architecture by freeze-fracture electron microscopy.
Journal of bacteriology 1994 Jan;176(1):21-31
-
Induction of protective immunity to Haemophilus ducreyi in the temperature-dependent rabbit model of experimental chancroid.
Journal of immunology (Baltimore, Md. : 1950) 1994 Jan;152(1):184-92
-
Isolation of integral membrane proteins by phase partitioning with Triton X-114.
Methods in enzymology 1994 Jan;228182-93
-
Solid-phase synthesis of biologically active lipopeptides as analogs for spirochetal lipoproteins.
Peptide research 1994 Jan;7(2):91-7
-
Southwestern Internal Medicine Conference: brucellosis: don't let it get your goat!
The American journal of the medical sciences 1994 Jan;307(1):64-75
-
Lipid modification of the 17-kilodalton membrane immunogen of Treponema pallidum determines macrophage activation as well as amphiphilicity.
Infection and immunity 1993 Apr;61(4):1202-10
-
Risk of acquisition of genital herpes simplex virus type 2 in sex partners of persons with genital herpes: a prospective couple study.
The Journal of infectious diseases 1993 Apr;167(4):942-6
-
Evaluation of molecular methodologies and rabbit infectivity testing for the diagnosis of congenital syphilis and neonatal central nervous system invasion by Treponema pallidum.
The Journal of infectious diseases 1993 Jan;167(1):148-57
-
Use of electroporation to construct isogenic mutants of Haemophilus ducreyi.
Journal of bacteriology 1992 Aug;174(16):5442-9
-
The outer membrane, not a coat of host proteins, limits antigenicity of virulent Treponema pallidum.
Infection and immunity 1992 Mar;60(3):1076-83
-
Localization of outer surface proteins A and B in both the outer membrane and intracellular compartments of Borrelia burgdorferi.
Journal of bacteriology 1991 Dec;173(24):8004-8
-
Lipoproteins of Borrelia burgdorferi and Treponema pallidum activate cachectin/tumor necrosis factor synthesis. Analysis using a CAT reporter construct.
Journal of immunology (Baltimore, Md. : 1950) 1991 Sep;147(6):1968-74
-
A temperature-dependent rabbit model for production of dermal lesions by Haemophilus ducreyi.
The Journal of infectious diseases 1991 Aug;164(2):359-67
-
Use of polymerase chain reaction and rabbit infectivity testing to detect Treponema pallidum in amniotic fluid, fetal and neonatal sera, and cerebrospinal fluid.
Journal of clinical microbiology 1991 Aug;29(8):1711-8
-
Sensitive detection of Treponema pallidum by using the polymerase chain reaction.
Journal of clinical microbiology 1991 Jan;29(1):62-9
-
Lipid modification of the 15 kiloDalton major membrane immunogen of Treponema pallidum.
Molecular microbiology 1990 Aug;4(8):1371-9
-
Expression in Escherichia coli of the 37-kilodalton endoflagellar sheath protein of Treponema pallidum by use of the polymerase chain reaction and a T7 expression system.
Infection and immunity 1990 Jul;58(7):2025-34
-
Immunogenic integral membrane proteins of Borrelia burgdorferi are lipoproteins.
Infection and immunity 1990 Apr;58(4):983-91
-
The 34-kilodalton membrane immunogen of Treponema pallidum is a lipoprotein.
Infection and immunity 1990 Feb;58(2):384-92
-
Molecular cloning and characterization of the 15-kilodalton major immunogen of Treponema pallidum.
Infection and immunity 1989 Dec;57(12):3708-14
-
Molecular characterization of the pathogen-specific, 34-kilodalton membrane immunogen of Treponema pallidum.
Infection and immunity 1989 Nov;57(11):3314-23
-
Molecular cloning and DNA sequence analysis of the 37-kilodalton endoflagellar sheath protein gene of Treponema pallidum.
Infection and immunity 1989 Nov;57(11):3403-11
-
Structural and antigenic conservation of the P2 porin protein among strains of Haemophilus influenzae type b.
Infection and immunity 1989 Nov;57(11):3270-5
-
Cardiovascular syphilis.
The American journal of medicine 1989 Oct;87(4):425-33
-
Acylation of the 47-kilodalton major membrane immunogen of Treponema pallidum determines its hydrophobicity.
Infection and immunity 1989 Sep;57(9):2878-85
-
Effect of primary immunization on pulmonary clearance of nontypable Haemophilus influenzae.
American journal of respiratory cell and molecular biology 1989 Sep;1(3):201-10
-
Major integral membrane protein immunogens of Treponema pallidum are proteolipids.
Infection and immunity 1989 Sep;57(9):2872-7
-
Antigenic evidence for simultaneous expression of two different lipooligosaccharides by some strains of Haemophilus influenzae type b.
Infection and immunity 1989 Jul;57(7):1971-8
-
Penicillin-binding proteins and peptidoglycan of Treponema pallidum subsp. pallidum.
Infection and immunity 1989 Apr;57(4):1248-54
-
Outer membrane ultrastructure explains the limited antigenicity of virulent Treponema pallidum.
Proceedings of the National Academy of Sciences of the United States of America 1989 Mar;86(6):2051-5
-
Sequence analysis of the 47-kilodalton major integral membrane immunogen of Treponema pallidum.
Infection and immunity 1989 Jan;57(1):196-203
-
Pathogen specificity of Treponema pallidum subsp. pallidum integral membrane proteins identified by phase partitioning with Triton X-114.
Infection and immunity 1988 Jul;56(7):1825-8
-
Immunization of rabbits with recombinant Treponema pallidum surface antigen 4D alters the course of experimental syphilis.
Journal of immunology (Baltimore, Md. : 1950) 1988 Apr;140(7):2415-21
-
Identification and localization of integral membrane proteins of virulent Treponema pallidum subsp. pallidum by phase partitioning with the nonionic detergent triton X-114.
Infection and immunity 1988 Feb;56(2):490-8
-
Unusual manifestations of secondary syphilis and abnormal humoral immune response to Treponema pallidum antigens in a homosexual man with asymptomatic human immunodeficiency virus infection.
Journal of the American Academy of Dermatology 1988 Feb;18(2 Pt 2):423-8
-
Genetic and physicochemical characterization of the recombinant DNA-derived 47-kilodalton surface immunogen of Treponema pallidum subsp. pallidum.
Infection and immunity 1988 Jan;56(1):71-8
-
Cloning and expression in Escherichia coli of the gene encoding the heat-modifiable major outer membrane protein of Haemophilus influenzae type b.
Infection and immunity 1987 Dec;55(12):2993-3000
-
Role of disulfide bonds in the oligomeric structure and protease resistance of recombinant and native Treponema pallidum surface antigen 4D.
Journal of bacteriology 1987 Apr;169(4):1365-71
-
Human IgG antibodies to Pseudomonas aeruginosa core lipopolysaccharide determinants are detected in chronic but not acute pseudomonas infection.
Scandinavian journal of infectious diseases 1987 Jan;19(6):649-60
-
Antigenic interrelationship between endoflagella of Treponema phagedenis biotype Reiter and Treponema pallidum (Nichols): molecular characterization of endoflagellar proteins.
Infection and immunity 1986 Dec;54(3):626-34
-
The antigenic interrelationship between the endoflagella of Treponema phagedenis biotype Reiter and Treponema pallidum Nichols strain. I. Treponemicidal activity of cross-reactive endoflagellar antibodies against T. pallidum.
Journal of immunology (Baltimore, Md. : 1950) 1986 Nov;137(9):2973-9
-
Correlation of treponemicidal activity in normal human serum with the presence of IgG antibody directed against polypeptides of Treponema phagedenis biotype Reiter and Treponema pallidum, Nichols strain.
Journal of immunology (Baltimore, Md. : 1950) 1986 Sep;137(6):2031-6
-
Serodiagnosis of syphilis by enzyme-linked immunosorbent assay with purified recombinant Treponema pallidum antigen 4D.
The Journal of infectious diseases 1986 Jun;153(6):1023-7
-
Native surface association of a recombinant 38-kilodalton Treponema pallidum antigen isolated from the Escherichia coli outer membrane.
Infection and immunity 1986 May;52(2):586-93
-
The surface of virulent Treponema pallidum: resistance to antibody binding in the absence of complement and surface association of recombinant antigen 4D.
Infection and immunity 1986 May;52(2):579-85
-
Properties of an ordered ring structure formed by recombinant Treponema pallidum surface antigen 4D.
Journal of bacteriology 1986 Mar;165(3):732-9
-
Purification and characterization of a cloned protease-resistant Treponema pallidum-specific antigen.
Infection and immunity 1984 Nov;46(2):598-607
-
Host-specific functional compartmentalization within the oligopeptide transporter during the Borrelia burgdorferi enzootic cycle
PLoS Pathogens 17e1009180
Books
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Lyme disease and relapsing fever spirochetes. Genomics, Molecular Biology, Host Interactions, and Disease Pathogenesis. 2021 Jan;
Book Chapters
-
Lyme Disease in Humans.
Current issues in molecular biology 2020 Dec;333-384
-
The Treponema pallidum Outer Membrane.
Current topics in microbiology and immunology 2017 Aug;1-38
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Borrelia: Molecular Biology, Host Interaction and Pathogenesis
Lyme disease in humans (Chapter 18). 2009 Jan;487-533
-
Manual of Clinical Microbiology, (Chapter 57)
Treponema and Brachyspira, human host-associated spirochetes. 2009 Jan;
-
Sexually Transmitted Diseases
Congenital Syphilis (Chapter 82) 2008 Jan;
-
Chapter 10 - Treponema: Molecular and Cellular Biology
Pathogenesis of Syphilis. Chapter 10. 2006 Jan;
-
Chapter 13 - Treponema: Molecular and Cellular Biology
Immunology of Syphilis. Chapter 13. 2006 Jan;
-
Chapter 4 - Treponema: Molecular and Cellular Biology
Metabolism of the Treponema,Chapter 4. 2006 Jan;
-
Molecular Biology of Spirochetes.
Use of green fluorescent protein transcriptional reporters to study differential gene expression by Borrelia burgdorferferi. 2006 Jan;
-
Infectious Diseases
Management of an HIV-positive pregnant woman with a positive VDRL test from an area endemic for Treponema infection 2002 Jan;
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The Prokaryotes: An Evolving Electronic Resource for the Microbiological Community
The Genus Borrelia. 2002 Jan;
-
Infectious Diseases
Management of a positive VDRL in HIV-infected pregnant women from areas endemic for non-venereal treponematoses (Chapter 56). 1999 Jan;
-
Sexually Transmitted Diseases
Congenital Syphilis (Chapter 84) 1999 Jan;
-
Medical Microbiology
Treponema (Chapter 36) 1996 Jan;
-
Atlas of Infectious Diseases. Volume II. GE Mandell (Editor-in-Chief) and DL Stevens (Editor). Churchill Livingstone, Philadelphia, PA.
Spirochetal Infections of the Skin. (Chapter 12). 1995 Jan;
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Opportunistic Complications of HIV (Sexually Transmitted Diseases)
Syphilis in the setting of HIV disease: opportunistic infection or fellow traveler? 1995 Jan;13-24
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Conn's Current Therapy.
Syphilis. 1994 Jan;699-702
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Infectious Diseases: A Treatise of Infectious Processes.
Syphilis. Chapter 73. 1994 Jan;694-714
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Diagnostic Molecular Microbiology: Principles and Applications.
PCR Detection of Treponema pallidum 1993 Jan;
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Lyme Disease: Molecular and Immunologic Approaches
Spirochete Molecular Architecture and Lyme Disease Pathogenesis. 1992 Jan;119-134
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Textbook of Internal Medicine.
Syphilis (Chapter 291). 1991 Jan;
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Lyme disease in humans
Lyme Disease and Relapsing Fever Spirochetes: Genomics, Molecular Biology, Host Interactions and Disease Pathogenesis
Conference Papers
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CD14 signaling restrains chronic inflammation through induction of p38-MAPK/SOCS-dependent tolerance.
PLoS pathogens 2009 Dec;5(12):e1000687
Editorials
-
T-Cell Responses to Treponema pallidum Proteins in Blood and Skin to Advance Syphilis Vaccine Design: Learning From Nature.
The Journal of infectious diseases 2024 Aug;230(2):275-277
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Editorial: Syphilis infection: clinical, epidemiology, basic science, and behavioral research.
Frontiers in immunology 2023 Jan;141182069
-
Posttreatment chronic Lyme disease--what it is not.
The Journal of infectious diseases 2005 Sep;192(6):948-9
Letters
-
Analysis of a flawed double-blind, placebo-controlled, clinical trial of patients claimed to have persistent Lyme disease following treatment.
Minerva medica 2009 Apr;100(2):171-2
-
Chemoprophylaxis against Lyme disease
The Lancet Infectious Diseases 2008 Jan;8(3):146-147
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Anti-tumor necrosis factor-alpha activation of Borrelia burgdorferi spirochetes in antibiotic-treated murine Lyme borreliosis: an unproven conclusion.
The Journal of infectious diseases 2007 Dec;196(12):1865-6; author reply 1866-7
-
Single-dose prophylaxis against Lyme disease.
The Lancet. Infectious diseases 2007 Jun;7(6):371-3
Notes
-
Who is the BosR around here anyway?
Molecular microbiology 2009 Dec;74(6):1295-9
-
Treponema pallidum, the stealth pathogen, changes, but how?
Molecular microbiology 2009 Jun;72(5):1081-6
-
MicroCommentary: Who is the BosR around here, anyway?
Mol Micro 2009 Jan;741295-1299
-
MicroCommentary: Treponema pallidum, the stealth pathogen, changes, but how?
Mol Micro 2009 Jan;721081-1086
-
The long strange trip of Borrelia burgdorferi outer-surface protein C.
Molecular microbiology 2008 Jul;69(1):1-4
Reviews
-
Syphilis.
Nature reviews. Disease primers 2017 Oct;317073
-
Treponema pallidum, the syphilis spirochete: making a living as a stealth pathogen.
Nature reviews. Microbiology 2016 Oct;14744-759
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Expert Review on: Biological false-positive tests comprise a high proportion of Venereal Disease Research Laboratory reactions in an analysis of 300,000 sera.
Geusau A, Kittler H, Hein U, Dangl-Erlach E, Stingl G, Tschachler
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Int J STD AIDS 2005 Jan;16722-726
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Review of “Bull’s Eye: Unraveling the Medical Mystery of Lyme Disease”
J Clin Invest 2004 Jan;1131378
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The immune response to infection with Treponema pallidum, the stealth pathogen.
Microbes and infection / Institut Pasteur 2002 Sep;4(11):1133-40
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Borrelia burgdorferi gene expression profiling with membrane-based arrays.
Methods in enzymology 2002 Jan;358165-77
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Treponema pallidum: doing a remarkable job with what it’s got
Trends Microbiol 1999 Jan;77-9
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Molecular approaches to improved syphilis serodiagnosis.
Serodiag. Immunother Infect Dis 1989 Jan;3299-306
Title or Abstract | Type | Sponsor/Event | Date/Year | Location |
---|---|---|---|---|
From the front lines: update on Lyme disease. | Talk | Pediatric Grand Rounds | 2017 | Connecticut Children's Medical Center |
Insights into the evolution of syphilis spirochetes within at-risk populations: Sequence variation of outer membrane protein ß-barrel domains in clinical samples. | Talk | STI and HIV World Congress | 2017 | Rio de Janeiro, Brazil |
The ongoing quest for T. pallidum outer membrane proteins: vaccines as the new frontier. | Talk | NIAID Workshop on the Development of a Syphilis Vaccine | 2017 | |
Grant writing. | Talk | UNC-South China Colloquium on STI Research 2016, Future Collaborations | 2016 | Guangzhou, China |
Outer membrane proteins of Treponema pallidum and syphilis vaccine development. | Talk | UNC-South China Colloquium on STI Research 2016, Future Collaborations | 2016 | Guangzhou, China |
Syphilis Clinical Immunology”, Writing an NIH Grant, and Syphilis basic immunology: Explaining the duality of syphilis. | Talk | First South China-UNC Advanced STI and Third South China-UNC STI Forum | 2014 | Guangzhou, China |
Unmasking the stealth pathogen: characterization of Treponema palladium rare outer membrane proteins. | Talk | International Union of Microbiological Societies | 2014 | Montreal, Canada |
From the front lines: Update on Lyme Disease. | Talk | Department of Medicine Grand Rounds | 2013 | University of Connecticut Health Center |
Molecular architecture of Treponema pallidum, the stealth pathogen. | Talk | Second South China-UNC Sexually Transmitted Infections Academic Forum | 2013 | Guangzhou, China |
From the front lines: Update on Lyme disease. | Talk | Middlesex Hospital, Grand Rounds | 2013 | Middletown, CT |
My 20+ years of (mostly) successful grant writing. | Talk | Seminar | 2012 | Connecticut Children's Medical Center |
Molecular architecture of Treponema pallidum: making a living as a stealth pathogen. | Talk | Department of Microbiology and Immunology | 2012 | University of Oklahoma Health Sciences Center |
Molecular architecture of Treponema pallidum, the stealth pathogen: a translational perspective. | Talk | Pediatric Translational Research Seminar | 2012 | University of Connecticut Health Center |
The going and coming of Lyme disease: differential gene expression, environmental sensing, and spirochete tracking | Talk | Department of Microbiology and Immunology | 2011 | University of Calgary, Calgary, CA. |
The going and coming of Lyme disease: differential gene expression, environmental sensing, and spirochete tracking | Talk | Inter-city Infectious Diseases Conference | 2011 | University of Connecticut Health Center. |
Tracking spirochetes and spirochete gene expression: illuminating the pathogenesis of Lyme disease | Talk | Department of Microbiology, LSU Veterinary School | 2010 | Baton Rouge, LA |
Molecular architecture of Treponema pallidum: making a living as a stealth pathogen | Talk | Department of Pathology and Laboratory Medicine | 2010 | UT Houston Medical Center. |
Tracking spirochetes and spirochete gene expression: illuminating the pathogenesis of Lyme disease | Talk | Department of Microbiology and Immunology | 2009 | University of Kentucky, Lexington, KY |
Immune recognition of Borrelia burgdorferi, the Lyme disease spirochete by phagosomal signaling: signaling from the grave | Talk | Department of Microbiology and Immunology | 2009 | U. of MD School of Medicine, Baltimore, MD. |
Tracking spirochetes and spirochete gene expression: illuminating the pathogenesis of Lyme disease | Talk | Department of Microbiology and Molecular Genetics | 2009 | Stony Brook University, Stony Brook, NY |
Tracking spirochetes and spirochete gene expression: illuminating the pathogenesis of Lyme disease | Talk | Department of Oral Biology, School of Dental Medicine | 2009 | SUNY at Buffalo, Buffalo, NY |
The Quest for Treponema pallidum outer membrane proteins: a reappraisal | Talk | Eighth Gordon Research Conference on the Biology of Spirochetes | 2008 | Ventura, CA. |
Immune recognition of pathogenic spirochetes by phagosomal signaling | Talk | Department of Immunology | 2008 | UCHC, Farmington, CT. |
Immune recognition of pathogenic spirochetes by phagosomal signaling | Talk | Forsyth Institute | 2008 | Harvard School of Dental Medicine, Boston, MA. |
Summary/Highlights: Basic Science Track | Talk | 17th meeting of the ISSTDR/10th IUSTI World Congress | 2007 | Seattle, WA |
Treponema pallidum, the syphilis spirochete: making a living as a stealth pathogen | Talk | Department of Microbiology & Immunology Research Seminar | 2007 | Med. Coll. VA., Richmond, VA. |
Treponema pallidum, the syphilis spirochete: making a living as a stealth pathogen | Talk | Department of Microbiology & Immunology Research Seminar | 2007 | Virginia Commonwealth University, Richmond, VA |
Spirochetes and spirochetal diseases | Talk | Medical College of Virginia | 2007 | Virginia Commonwealth University, Richmond, VA. |
Outer membrane proteins of Treponema pallidum, the syphilis spirochete | Talk | Hauptman-Woodward Institute | 2007 | Buffalo, NY |
Recognition of pathogenic spirochetes by phagosomal signaling | Talk | Department of Veterinary and Animal Sciences | 2007 | University of Massachusetts, Amherst, MA. |
Microbial Pathogenesis: a new frontier in translational research | Talk | Connecticut Children’s Medical Center | 2007 | Hartford, CT |
Syphilis Pathogenesis: a post-genomics perspective | Talk | Infectious Diseases Grand Rounds | 2007 | New York Medical College, Valhalla, NY. |
The Treponema pallidum outer membrane and what lies beneath | Talk | Symposium on Pathogenic Spirochetes | 2006 | UCLA Dept. Micro., Immunology, and Molecular Gen. |
Immune recognition by pathogenic spirochetes: looking below the surface | Talk | Department of Medicine Research Seminar | 2006 | U. of Mass.Medical School, Worcester, MA. |
Syphilis and Syphilis Research Sponsored by Drs. Socrates Herrara and Miriam Arevalo, Instituto de Inmunologia del Valle. | Talk | students and faculty at Universidad del Valle | 2005 | Cali Colombia |
Innate and Adaptive Immunity: An Infectious Disease Doc’s Perspective Sponsored by Corporacion de Lucha Contra El SIDA, Cali Colombia. | Talk | X Curso Internacional de Enfermedades Infecciosas | 2005 | Cali Colombia |
Innate and Adaptive Immunity in Syphilis and Lyme Disease (Dr. Carol Wu, organizer) | Talk | UCHC Faculty Research Seminar Series | 2005 | UCHC |
Syphilis Research in the 21st Century: One Investigator’s Perspective | Talk | 2005 | Walter Reed Army Inst. for Res., Rockville, MD. | |
Syphilis Research in the 21st Century: One Investigator’s Perspective | Talk | CIDEIM | 2005 | Cali, Colombia. |
Infectious Diseases in the Post-Genomics Era Sponsored by Corporacion de Lucha Contra El SIDA | Talk | XI Seminario Integral del Sida y | 2005 | Cali Colombia |
Use of green fluorescent protein transcriptional reporters to study differential gene expression by Borrelia burgdorferi | Talk | NATO Advanced Research Workshop on the Molecular Biology of Spirochetes | 2005 | Prague, Czech Republic |
The Innate-Adaptive Immune Interface in Syphilis and Lyme disease | Talk | 2004 | Albany Medical College, Albany, NY. | |
Innate and adaptive immunity: an introduction | Talk | CIDEIM | 2004 | Cali, Colombia |
Syphilis-HIV Interactions: What we do and don’t know | Talk | IX Curso Internacional de Enfermedades Infecciosas | 2004 | X Seminario Integral del Sida y |
Differential gene expression by Borrelia burgdorferi: new tools and new paradigms | Talk | Department of Microbiology and Molecular Genetics | 2003 | University of Dentistry and Medicine, New Jersey |
20 years of syphilis research | Talk | Centro Internacional de Entrenamiento e Investigaciones Medicas (CIDIEM), | 2003 | Cali, Colombia. |
Chaired a session on host-pathogen interactions and presented an overview entitled “Lipoproteins, innate immunity, and the cutaneous immune response to Borrelia burgdorferi” | Talk | Fifth Gordon Research Conference on the Biology of Spirochetes | 2002 | Ventura, CA. |
Differential expression by Borrelia burgdorferi and shuttle vector development: what goes around, comes around | Talk | Wadsworth Public Health Laboratories | 2002 | Albany, NY |
Organized and convened a colloquium entitled Arthropod-vector interactions: the flip-side of human disease | Talk | 102nd General Meeting of the American Society for Microbiology | 2002 | Salt Lake City, Utah. |
Molecular pathogenesis of Lyme disease | Talk | Ninth International Conference on Lyme Borreliosis and other Tick-borne Diseases | 2002 | New York, NY. |
The roll of toll-like receptors in innate inflammatory and immune responses to spirochetal infections | Talk | XXVIIth meeting of the Brazilian Society of Immunology | 2002 | Salvador, Brazil |
Spirochetal lipoproteins as proinflammatory mediators in syphilis and Lyme disease | Talk | Dental Dean’s Seminar | 2001 | University of Connecticut Health Center. |
TLR2 responses in vivo: bridging innate and adaptive immunity (Dr. D. Golenbock, organizer). | Talk | Toll-receptor club | 2001 | Boston University Medical Center |
Spirochetal lipoproteins as proinflammatory agonists in syphilis and Lyme disease | Talk | Division of Rheumatology | 2001 | New England Medical Center, Boston, MA. |
B. burgdorferi lipoproteins and innate immunity in Lyme disease | Talk | NIH-sponsored workshop on neuroborreliosis | 2001 | Airlie Center, Virginia |
Development of a Borrelia burgdorferi shuttle vector: turning the tables | Talk | Department of Molecular and Cellular Biology | 2001 | University of Connecticut, Storrs |
Spirochetal lipoproteins as proinflammatory agonists in syphilis and Lyme disease | Talk | Rheumatology Grand Rounds | 2000 | Yale University School of Medicine, New Haven, CT. |
Structure-function relationships of Treponema pallidum membrane proteins | Talk | Fourth Gordon Research Conference on the Biology of Spirochetes | 2000 | Ventura, California |
Treponema pallidum and Syphilis Research: lessons from the genome | Talk | UCHC GMB&B Faculty Research Seminar Series | 2000 | |
Treponema pallidum and syphilis research: Lessons from the genome | Talk | Department of Microbiology | 2000 | Indiana U. School of Medicine, Indianapolis, IN. |
Spirochetal lipoproteins as proinflammatory agonists in syphilis and Lyme disease | Talk | Division of Infectious Diseases | 2000 | Indiana U. School of Medicine, Indianapolis, IN. |
Molecular architecture of Treponema pallidum: implications for syphilis pathogenesis. Keynote address. | Talk | annual meeting of the Center for Excellence in Vaccine Research at UConn | 2000 | Storrs, CT |
Molecular architecture of Treponema pallidum: implications for syphilis pathogenesis | Talk | Depts. of Biochemistry and Microbiology | 1999 | West Virginia U., Morgantown, W.Va. |
Spirochetal lipoproteins as proinflammatory agonists in syphilis and Lyme disease | Talk | Infectious Diseases Division | 1999 | Boston University School of Medicine |
Molecular architecture of Treponema pallidum: implications for syphilis pathogenesis | Talk | Oswaldo Cruz Foundation | 1999 | Salvador, Brazil. |
Syphilis Pathogenesis and New Developments in Syphilis Diagnosis | Talk | Brazilian Congress of Microbiology | 1999 | Salvador, Brazil |
Molecular architecture of Borrelia burgdorferi: implications for Lyme Disease pathogenesis | Talk | Dept. of Microbiology and Internal Medicine | 1998 | UCHC, Farmington, CT. |
Molecular architecture of Borrelia burgdorferi: implications for Lyme Disease pathogenesis | Talk | Dept. of Biology and Dept. of Internal Medicine | 1998 | Brookhaven Nat. Lab. and SUNY at Stony Brook, NY. |
Treponema pallidum biology: doing the best it can with what it’s got and Pathogenesis of syphilis (also served as co-organizer and co-Chair). | Talk | International Business Conference-sponsored symposium on Spirochete Diseases | 1998 | |
New model systems for studying mammalian host adaptation by Borrelia burgdorferi | Talk | seminar to the Depts. of Molecular Biology and Entomology | 1998 | Oklahoma State University, Stillwater, OK. |
Identification of rare outer membrane proteins in isolated Treponema pallidum outer membranes - a reappraisal | Talk | Third Conference on the Biology of Spirochetes | 1998 | Ventura, CA |
Syphilis Pathogenesis | Talk | graduate student course on bacterial pathogenesis | 1998 | NYU Skirball Institute |
New model systems for studying differential gene expression by Borrelia burgdorferi | Talk | 1998 | Skirball Institute, NYU School of Medicine, NYC. | |
Treponema pallidum molecular architecture: implications for syphilis pathogenesis and A new model system for studying differential gene expression by Borrelia burgdorferi | Talk | Dept. of Microbiology and Immunology | 1998 | Medical College of Virginia. |
Spirochetal Lipoproteins: Structure-function relationships and host responses Presented as part of a symposium on "Membrane-anchored Molecules" | Talk | Annual Meeting of the Society for General Microbiology | 1997 | Reading, UK. |
Spirochetal lipoproteins as inflammatory mediators in syphilis and Lyme disease | Talk | Division of Infectious Diseases/Dept. of Microbiology & Immunology seminar | 1997 | University of North Carolina, Chapel Hill |
A new animal model for studying Borrelia burgdorferi in the mammalian host-adapted state | Talk | Department of Biochemistry & Molecular Biology | 1997 | New York Medical College, Valhalla, NY. |
A vaccine for syphilis: the never ending story Presented as part of a symposium entitled "Vaccines for Sexually Transmitted Diseases" | Talk | 37th Interscience Conference on Antimicrobial Agents and Chemotherapy (ICAAC), | 1997 | Toronto, CA |
Spirochetal lipoproteins as inflammatory mediators in syphilis and Lyme disease | Talk | Division of Infectious Diseases/Department of Microbiology seminar | 1997 | LA State U. School of Medicine, New Orleans, LA. |
Outer membrane ultrastructure as a virulence determinant of Treponema pallidum and Borrelia burgdorferi | Talk | Second Gordon Conference on the Biology of Spirochetes | 1996 | Ventura, CA |
Lyme Disease 1996: the clouds are parting | Talk | McBee Lectureship | 1996 | Baylor Medical Center |
Lyme Disease 1996 | Talk | Dermatology Grand Rounds | 1996 | UT Southwestern and St. Paul's Medical Centers |
Treponema pallidum molecular architecture: Implications for disease pathogenesis and vaccine development | Talk | Infectious Diseases Research Seminar Series | 1996 | Univ. of Cincinatti, Cincinatti, OH. |
Syphilis and HIV: results of a prospective, multicenter, CDC sponsored study of syphilis and HIV infection Sponsored by the Division of Infectious Diseases, UT Southwestern Medical Center and the Dallas County Public Health Department. | Talk | Sexually Transmitted Diseases Seminar Series | 1996 | UT Southwestern Medical Center |
Borrelia burgdorferi molecular Architecture and Lyme disease pathogenesis | Talk | Division of Infectious Diseases seminar | 1995 | SUNY at Downstate Medical Center |
Syphilis and rare outer membrane proteins | Talk | Keystone Symposium entitled "Sexually Transmitted Diseases in the HIV Era" | 1995 | Keystone, Colorado |
Treponema pallidum molecular architecture: Implications for syphilis pathogenesis | Talk | 1995 | SUNY at Stony Brook | |
The Molecular biology of Treponema pallidum: Where next? | Talk | International Society for STD Research | 1995 | New Orleans, LA |
Outer membrane ultrastructure as a major virulence factor of Treponema pallidum | Talk | First Gordon Conference on the Biology of Spirochetes | 1994 | Ventura, CA. |
Syphilis in the HIV Era | Talk | International Society for Infectious Diseases | 1994 | Prague, Czech Republic |
Lyme Disease 1993: of mice and men. Presented at the annual meeting | Talk | Oklahoma Infectious Diseases Society | 1993 | Oklahoma |
Surface components of Borrelia burgdorferi Sponsored by the Lyme Borreliosis Foundation and the Stamford, CT Dept. of Public Health. | Talk | 1992 | Stamford, CT | |
Treponema pallidum uItrastructure: Implications for syphilis pathogenesis. | Talk | annual meeting of the Texas chapter of the American Society of Microbiology | 1992 | San Antonio, TX |
New Insights into the molecular architecture of Borrelia burgdorferi | Talk | symposium on the Immunobiology of Lyme Disease | 1992 | Cold Spring Harbor, NY. |
Immunology and molecular biology of spirochetal lipoproteins | Talk | First Cold Spring Harbor Laboratory Symposium on the Immunobiology of Lyme Disease | 1991 | Cold Spring Harbor, N.Y. |
Ultrastructure and molecular biology of Treponema pallidum: Implications for syphilis pathogenesis | Talk | Annual Meeting of the American for Microbiology | 1991 | |
Triton X-114 phase partitioning of spirochetal membrane proteins | Talk | 7th International Conference on Partitioning in Aqueous Two-Phase Systems | 1991 | New Orleans, LA. |
Participant and presented a synopsis of my research on T. pallidum molecular biology and ultrastructure. | Talk | NIH-sponsored symposium entitled "The Molecular Immunology of Sexually Transmitted Diseases" | 1991 | Rocky Mountain National Laboratories, Hamilton, MN |
Molecular immunology of spirochetal lipoproteins | Talk | NIH-sponsored symposium on Lyme disease | 1991 | Rocky Mnt. National Lab., Hamilton, MT |
Invited participant(unable to attend). | Talk | Molecular Biology of Spirochetes symposium | 1991 | Annecy, France |
Spirochete ultrastructure and molecular biology: Implications for pathogenesis | Talk | Division of Infectious Diseases seminar | 1991 | Vanderbilt University, Nashville, TN. |
Moderator Molecular biology of pathogens | Other | Annual Meeting of the American Society for Microbiology | 1990 | Anaheim, CA. |
Syphilis and HIV Infection and Lyme disease 1990 | Talk | Annual meeting of the Texas Infectious Diseases Society | 1990 | Bandera, TX. |
Developing a molecular biology of syphilis | Talk | Infectious Diseases seminar | 1990 | Brown University, Providence, R.I. |
Invited participant | Talk | NIH symposium on Lyme disease | 1990 | Bethesda, MD. |
Spirochete lipoproteins: Molecular biology and Implications for disease pathogenesis | Talk | Department of Microbiology | 1990 | University of Alabama at Birmingham |
Molecular and ultrastructural Analysis of the Treponema pallidum outer membrane presented at a World Health Organization-sponsored symposium | Talk | The Biology and the Pathogenicity of Treponemes | 1989 | University of Birmingham, Birmingham, UK. |
Analysis of the Treponema pallidum outer membrane by freeze-fracture electron microscopy. Presented at a symposium entitled Special Topics in Modern Treponema Research | Talk | Annual Meeting of the American Society for Microbiology | 1989 | New Orleans, LA. |
Towards a molecular biology of Treponema pallidum | Talk | Sexually Transmitted Diseases Seminar, Harborview Medical Center | 1989 | University of Washington, Seattle, WA. |
Molecular biology of neurosyphilis | Talk | Medical Genetics Rounds | 1989 | UT Southwestern Medical Center |
Participant, panel discussion on Syphilis and HIV Infection | Talk | Centers for Disease Control | 1988 | Atlanta, GA. |
Syphilis 1987: Goodbye, Colombus. | Talk | Sexually Transmitted Diseases Seminar Series and Dallas County Public Health Department | 1987 | U.T. Southwestern Medical Center |